共被期刊论文引用了2次
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| 1 | Xihuang Pill(西黄丸) Induces Mesenchymal-Epithelial Transition and Inhibits Loss of Apical-Basal Polarity in Colorectal Cancer Cell through Regulating ZEB1-SCRIB Loop显示文摘Objective:To investigate the antiproliferative and anti-metastasis effect of Xihuang Pill(西黄丸,XP) on human colorectal cancer cell and to explore the molecular mechanism by which it produces the effects.Methods:Highly metastatic human colorectal cancer cell line LoVo was treated with low-,medium-,and highdose XP-containing serum(XP-L,XP-M,XP-H) groups for 48 h,cells intervened with no drug rat serum and PD98059[extracellular signal-regulated kinase(ERK) inhibitor]as negative and positive controls(NC and PC)groups.Cell proliferation assay was made using cell counting kit-8(CCK8).The 8 μm pore-size transwell chamber and 4',6-diamidino-2-phenylindole(DAPI) staining were applied to examine the ability of invasion and migration of the cells.The protein expression of ERK1/2,zinc finger E-box-binding homeobox 1(ZEB1),Scrib and lethal giant larvae homolog 2(Lgl2) was detected by Western blotting while the relative mRNA quantity of E-cadherin,N-cadherin,Occludin and junctional adhesion molecule-1(JAM1) was measured by realtime fluorescent quantitative polymerase chain reaction(RT-qPCR).Results:XP induced a dose-dependent suppression on the proliferation of LoVo cells(P<0.05 or P<0.01),with the inhibition rates varied from 27.30%to31.08%.Transwell assay showed that when preprocessed with PD98059 and XP-containing serum,the number of cells that passed the filter decreased significantly compared with that of NC group(P<0.05 or P<0.01).Moreover,XP inhibited the protein expression of ERK1/2 and ZEB1(P<0.05);and up-regulated the protein expression of Scrib and Lgl2(P<0.05).The mRNA levels of E-cadherin,Occludin and JAM1 of the XP intervened groups and PC group markedly ascended(P<0.05) while that of N-cadherin showed a descending tendency(P>0.05).Conclusion:XP intervention suppressed the ability of proliferation,invasion and migration of the LoVo cells.Regulating ZEB1-SCRIB Loop so as to recover epithelial phenotype and apical junctional complex might be one of the mechanisms by which XP produces the anti-metastasis effect. | 王邈 孟静岩 何俗非 | 2014 | Chinese Journal of Integrative Medicine2014，20，10： | 20 |
| 2 | ZEB1-SCRIB环路与肿瘤转移显示文摘0引言
上皮-间质转化(epithelial-mesenchymal transitions,EMT),是指上皮细胞失去极性,经过细胞骨架重塑,转变成具有迁移能力的间充质表型的过程,发生EMT的细胞主要表现为细胞变梭、伸出丝状伪足并获得侵袭运动能力;在分子生物学方面表现为上皮标志物E-cadherin(E-钙连蛋白)的缺失或减弱,而获得间质标志物, | 王邈 孟静岩 | 2013 | 肿瘤防治研究2013，40，3： | 1 |
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