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| 1 | Baicalin modulates microRNA expression in UVB irradiated mouse skin显示文摘This study aimed to evaluate the effects of baicalin on ultraviolet radiation B (UVB)-mediated microRNA (miRNA) expression in mouse skin.We determined miRNA expression profiles in UVB irradiated mice,baicalin treated irradiated mice,and untreated mice,and conducted TargetScan and Gene Ontology analyses to predict miRNA targets.Three miRNAs (mmu-miR-125a-5p,mmu-miR-146a,and mmu-miR-141) were downregulated and another three (mmu-miR-188-5p,mmu-miR-223 and mmu-miR-22) were upregulated in UVB irradiated mice compared with untreated mice.Additionally,these miRNAs were predicted to be related to photocarcinogenesis,hypomethylation and apoptosis.Three miRNAs (mmu-miR-378,mmu-miR-199a-3p and mmu-miR-181b) were downregulated and one (mmu-miR-23a) was upregulated in baicalin treated mice compared with UVB irradiated mice,and they were predicted to be related to DNA repair signaling pathway.These deregulated miRNAs are potentially involved in the pathogenesis of photodamage,and may aid treatment and prevention of UVB-induced dermatoses. | Yang Xu Bingrong Zhou Di Wu Zhiqiang Yin Dan Luo | 2012 | The Journal of Biomedical Research2012，26，2： | 3 |
| 2 | 中波紫外线诱导小鼠皮肤光损伤中miRNA141表达的研究显示文摘目的探讨中波紫外线(UVB)诱导光损伤小鼠皮肤中miRNAl41表达的变化。方法以C57/BL6小鼠为研究对象,实验分空白组及30、60、90、180、270mJ/cmzUVB剂量组,单次照射小鼠背部去毛皮肤,24h后取照射部位皮肤,采用实时定量PCR检测各组miRNAl41的表达水平,同时利用在线数据库TargetScan等预测靶基因,并用Gostat软件对其进行功能类聚分析,通过石蜡切片HE染色观察各组小鼠皮肤的病理学变化,并结合免疫组化技术检测人第10号染色体缺失的磷酸酶及张力蛋白同源物(PTEN)蛋白的表达水平。利用SPSS13.0软件进行统计学分析,组间差异比较采用方差分析。结果UVB照射后,空白组、30、60、90、180、270mJ/cm^2UVB各组miRNAl41的相对表达量分别为2.1354±0.4289、2.4333±0.2517、2.9328±0.3126、3.4125±0.3606、4.5667±0.4014、6.7428±0.5158,miRNAl41的表达水平与UVB照射剂量呈正相关(r=0.992,P〈0.01)。Gostat分析显示,miRNAl41下游调控的靶点基因主要与信号转导、转录调节等相关。免疫组化结果提示,随着UVB照射剂量的增高,PTEN蛋白的表达水平逐渐下降。结论miRNAl41可能在UVB诱导的光损伤机制中发挥作用,且可能与炎症反应有关,PTEN在急性光损伤的炎症反应中可能。 | 李巍 华丽娟 周炳荣 骆丹 | 2013 | 中华皮肤科杂志2013，46，12： | 0 |
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