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| 1 | Mechanistic links between gut microbial community dynamics, microbial functions and metabolic health显示文摘Gut microbes comprise a high density, biologically active community that lies at the interface of an animal with its nutritional environment. Consequently their activity profoundly influences many aspects of the physiology and metabolism of the host animal. A range of microbial structural components and metabolites directly interact with host intestinal cells and tissues to influence nutrient uptake and epithelial health. Endocrine, neuronal and lymphoid cells in the gut also integrate signals from these microbial factors to influence systemic responses. Dysregulation of these host-microbe interactions is now recognised as a major risk factor in the development of metabolic dysfunction. This is a two-way process and understanding the factors that tip host-microbiome homeostasis over to dysbiosis requires greater appreciation of the host feedbacks that contribute to regulation of microbial community composition. To date, numerous studies have employed taxonomic profiling approaches to explore the links between microbial composition and host outcomes(especially obesity and its comorbidities), but inconsistent host-microbe associations have been reported. Available data indicates multiple factors have contributed to discrepancies between studies. These include the high level of functional redundancy in host-microbiome interactions combined with individual variation in microbiome composition; differences in study design, diet composition and host system between studies; and inherent limitations to the resolution of r RNA-based community profiling. Accounting for these factors allows for recognition of the common microbial and host factors driving community composition and development of dysbiosis on high fat diets. New therapeutic intervention options are now emerging. | Connie WY Ha Yan Y Lam Andrew J Holmes | 2014 | World Journal of Gastroenterology2014，20，44： | 4 |
| 2 | What's hot in inflammatory bowel disease in 2011?显示文摘Ulcerative colitis and Crohn's disease(CD) are the two major forms of inflammatory bowel disease(IBD).In this highlight topic series of articles,the most recent advances in the IBD field are reviewed,especially the newly described cytokines,including the therapeutic implications for their manipulation.In addition,the interplay between the intestinal microbiota and the host is reviewed,including the role of defensins and dysbiosis in CD pathogenesis.Finally,the importance of the non immune systems such as endothelial cells and the hemostatic system are highlighted as new players in IBD pathogenesis. | Silvio Danese | 2011 | World Journal of Gastroenterology2011，17，5： | 1 |
| 3 | 人β-防御素-3与人表皮生长因子融合蛋白的克隆及原核表达显示文摘目的:克隆融合蛋白d-EGF成熟链基因,构建原核表达载体,并在大肠埃希菌(E.coli)表达体系中进行有效表达、分离纯化并鉴定其特异性。方法:以蛋白基因序列为基础,RT-PCR扩增人防御素-β和表皮生长因子基因,分别构建β-防御素-3(β-defensin-3)、EGF重组质粒,应用重组PCR方法,将EGF的DNA序列拼接到β-defensin-3 DNA序列的3'端,将基因克隆入原核表达载体pET-SUMO,构建重组质粒pET-SUMO-d-EGF。采用PCR、测序等方法鉴定后转化E.coli BL21(DE3),经IPTG诱导表达,Ni-trap柱纯化,SDS-PAGE检测表达及纯化结果,最后以Western blot对其进行特异性鉴定。结果:成功构建β-defensin-3、EGF重组质粒,经重组PCR成功扩增出294 bp的目的片段,重组体PCR结果与预期结果一致,测序正确。转入重组质粒的E.coli BL21(DE3)经IPTG诱导经SDS-PAGE分析得到28 kDa左右的目的蛋白条带。Western blot鉴定出融合蛋白含有抗EGF、β-防御素-3两种抗原特异性的蛋白。结论:成功构建原核表达载体pET-SUMO-d-EGF,并获得纯化的融合蛋白,为进一步的活性分析奠定了实验基础。 | 房兵 孙淼 李友建 武晓茜 耿家珍 邵先安 | 2012 | 蚌埠医学院学报2012，37，5： | 0 |
| 4 | 融合蛋白d-EGF的结构预测及其意义显示文摘目的对融合蛋白d-EGF二级、三级结构进行预测,为构建相应结构重组分子的可能性提供理论指导。方法以融合蛋白基因序列为基础。用DNAStat软件预测其蛋白质的二级结构;用InsightII软件对其三级结构进行建模预测。结果DNAStar软件的GamierRobson方法预测融合蛋白d-EGF含较多争折叠结构;ChargeDensity-Charge显示其3~22,31~48区段含丰富的正电荷。Insight II软件对其三级结构进行建模显示融合蛋白d-EGF存在一个由六个链内二硫键组成曲结构域,同时存在3个反向平行的β-折叠结构,这些结构是融合前β-defensin-3,EGF两分子生物学活性所必需的部分。结论融合蛋白d-EGF的二、三级结构预测结果显示该组合分子可能具有β-defensin-3,EGF两种分子的功能,该方法可以为构建具有相应结构重组蛋白的可能性提供理论基础。 | 房兵 孙淼 李友建 李娜 耿家珍 邵先安 | 2012 | 现代检验医学杂志2012，27，1： | 0 |
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