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| 1 | 柳属植物特性及其在污染底泥修复中的应用研究进展显示文摘污染底泥具有处置困难、污染严重并可引起上覆水体二次污染从而造成恶性循环等特点,植物修复是一种绿色环保、高效、对环境扰动少、易于被公众所接受的污染修复方式,柳属植物因具有生态幅宽以及易繁殖、生长快,尤其是较禾本科植物更加适合污染底泥修复的生物学特性,被选作植物修复材料而倍受关注.本文在介绍和分析柳属植物特性及其环境适应力的生态学意义的基础上,重点梳理其对污染物的修复方式与机理,包括植物萃取(Phytoextraction)、植物转化(Phytotransformation)、植物固定(Phytostabilization)、植物降解(Phytodegradation)和植物挥发(Phytovolatilization)等.进一步详述柳属植物在底泥修复中应用的国内外进展,在累积重金属、降解有机物和改变底泥性质等方面的作用研究说明其对污染底泥的修复具有很好的效果.最后结合目前柳属植物研究中的一些问题和应用实践,建议未来应更注重野外试验、延长试验周期、开展修复植物群落优化配置的研究等. | 杨天雪 何春光 罗文泊 王天驰 盛连喜 | 2015 | 应用与环境生物学报2015，21，5： | 5 |
| 2 | Fine mapping of the sex locus in Salix triandra confirms a consistent sex determination mechanism in genus Salix显示文摘Salix triandra belongs to section Amygdalinae in genus Salix,which is in a different section from the willow species in which sex determination has been well studied.Studying sex determination in distantly related willow species will help to clarify whether the sexes of different willows arise through a common sex determination system.For this purpose,we generated an intraspecific full-sib F1 population for S.triandra and constructed high-density genetic linkage maps for the crossing parents using restriction site-associated DNA sequencing and following a two-way pseudo-testcross strategy.With the established maps,the sex locus was positioned in linkage group XV only in the maternal map,and no sex linkage was detected in the paternal map.Consistent with previous findings in other willow species,our study showed that chromosome XV was the incipient sex chromosome and that females were the heterogametic sex in S.triandra.Therefore,sex in this willow species is also determined through a ZW sex determination system.We further performed fine mapping in the vicinity of the sex locus with SSR markers.By comparing the physical and genetic distances for the target interval encompassing the sex determination gene confined by SSRs,severe recombination repression was revealed in the sex determination region in the female map.The recombination rate in the confined interval encompassing the sex locus was approximately eight-fold lower than the genome-wide average.This study provides critical information relevant to sex determination in S.triandra. | Wei Li Huaitong Wu Xiaoping Li Yingnan Chen Tongming Yin | 2020 | Horticulture Research2020，7，1： | 3 |
| 3 | Multiallelic and multilocus simple sequence repeats(SSRs)to assess the genetic diversity of a Salix spp.germplasm collection显示文摘Salix L.(willow)is the largest genus of the family Salicaceae and plays an important role in riparian habitats,wetlands and in shrub tundra.Due to the diff erent implications for the species belonging to this family,it is fundamental to identify molecular tools characterizing relevant clones.A set of six multilocus and multiallelic simple sequence repeat(SSRs)markers are presented,leading to 390 polymorphic fragments considered as single dominant markers and able to discriminate successfully 92 S.alba L.from 24 Salix spp.The polymorphic fragments have been used to perform genetic diversity studies,and to investigate population structures and cluster analysis in a germplasm collection.The results highlight the capability of the six SSRs to be powerful genetic resources in applied forestry research,both to distinguish S.alba clones from Salix spp.and to perform genetic population studies for breeding programs. | Giorgia Carletti Luigi Cattivelli Lorenzo Vietto Giuseppe Nervo | 2021 | Journal of Forestry Research2021，32，1： | 1 |
| 4 | 柳树离体再生及遗传转化体系研究进展显示文摘柳树因其药用价值、生态修复能力及作为生物能源的潜力而受到关注。近些年已培育出许多优良品种,并在柳属植物基因组中定位到与经济性状及抗逆性相关的候选基因,建立离体再生及遗传转化体系可以获得大量优质柳树苗,并对候选基因进行功能研究。为了更清晰地认识影响柳树再生及遗传转化的不同因素,本文对柳树离体再生和遗传转化的研究进展进行了总结,主要包括柳属不同种初代培养、继代增殖培养、生根培养的最佳培养基的筛选,诱导愈伤组织最佳的培养基、激素、外植体类型的筛选,遗传转化时最佳的农杆菌的类型、浓度、侵染时间、预培养和共培养时间及选择压等因素的筛选。目前柳树的愈伤组织再生和遗传转化仍存在难点,本研究对其未来进一步深入研究方向进行了展望,以期对柳树的离体再生及遗传转化体系建立提供参考,为后续柳树基因工程研究及柳树的遗传改良奠定基础。 | 刘源 李际红 秦光华 刘翠双 孙茂桐 牛牧歌 王锦楠 | 2023 | 中国农学通报2023，39，29： | 0 |
| 5 | 新疆柳树花变叶植原体分子鉴定显示文摘为研究新疆7个地区柳树花变叶病为何种病原物所造成,本研究以分子生物学技术对其进行鉴定。通过CTAB法提取柳树总DNA,对其16S rRNA基因和tuf基因进行PCR扩增,将测序得到的基因片段通过Blast比对、同源性比较,构建系统进化树和16S rRNA虚拟RFLP分析。结果显示,PCR扩增分别获得16S rRNA(1238 bp)和tuf(824 bp)的片段,证明该病害与植原体有关,将其命名为柳树花变叶植原体(WPP);16S rRNA序列分析表明,WPP与中国桃黄化植原体(‘Prunus persica’yellows phytoplasma)、枣疯病('Ziziphus jujuba’witches'-broom phytoplasma)、杏褪绿卷叶植原体(Apricot leaf roll phytoplasma)同源性均为99.68%;16S rRNA虚拟RFLP分析结果表明,WPP与枣疯病株系(GenBank登录号:AB052876)有99.8%的相似性,虚拟RFLP模式与16SrV-B亚组的参考模式相同,相似系数为1.00,进一-步证明WPP为16SrV-B亚组成员。WPP tuf基因与榆树黄化组(EY)同源性达99%以上,系统进化树显示,柳树花变叶植原体与榆树黄化组(16SrV)植原体聚为--枝。柳树花变叶植原体属于16SrV.B.tuf-B亚组。 | 李丰 赖刚刚 赵志慧 张萍 朱天生 | 2022 | 西北农业学报2022，31，10： | 0 |
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