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| 1 | 植物TCP转录因子的作用机理及其应用研究进展显示文摘TCP转录因子是一类植物特有蛋白,含有保守的TCP domain,其中由60个氨基酸组成的b HLH结构是结合DNA和蛋白互作所必需的。TCP转录因子由于其广泛参与调控植物的生长发育过程(如分枝、株高、叶型、花型等)而备受关注。最近有报道显示,TCP转录因子在植物逆境胁迫应答中(如低温和高盐)同样发挥重要作用。TCP蛋白参与多种信号转导途径(如油菜素内酯、茉莉酸、赤霉素、细胞分裂素等),可能是连接生长发育和介导胁迫响应的一个交叉点。本文从分子生物学角度,系统综述了植物TCP转录因子的作用机理及其在激素应答、发育调控及环境胁迫响应等过程中的功能,以期为基因工程方法改良作物生长模式和抗性提供参考。 | 冯志娟 徐盛春 刘娜 张古文 胡齐赞 龚亚明 | 2018 | 植物遗传资源学报2018,19,1: | 13 |
| 2 | 我国观赏花卉品质形成的功能基因研究进展显示文摘花色、花香、花形和花期等品质性状与花卉商品价值体现有着直接的关系,花卉品质形成相关的功能基因研究十分重要。该文综述了近10年来我国观赏花卉品质形成相关的功能基因研究成果,以期为花卉分子育种研究提供理论参考。 | 王小菁 杨玉萍 | 2013 | 植物学报2013,48,5: | 8 |
| 3 | Functional Diversity of CYCLOIDEA-like TCP Genes in the Control of Zygomorphic Flower Development in Lotus japonicus显示文摘CYCLOIDEA (CYC)-like TCP genes play key roles in dorsoventral differentiation of zygomorphic flowers in Papilionoideae legumes. In this study, we analyzed the kew mutants whose flowers lost lateral identity, and investigated the diverse functions of three LjCYC genes during zygomorphic flower development in the model legume Lotus japonicus. We showed that kew1 and kew3 are allelic mutants of LjCYC3, a CYC-like TCP gene. Through transgenic experiments, it was shown that LjCYC1 possesses dorsal activity similar to LjCYC2, and that LjCYC3 alone is sufficient to confer lateral activity, and an epistatic effect between dorsal and lateral activities was identified. Sequence analysis revealed a striking alteration at the 3 end of the LjCYC3 open reading frame (ORF) in comparison with those of LjCYC1 and LjCYC2 ORFs. Furthermore, it was found that LjCYC proteins could interact with each other and possess different activities by means of a transcriptional activity assay. Our data demonstrate that the sequence variation and the subsequent alteration of protein property play important roles in the functional diversity of different LjCYC genes in controlling zygomorphic flower development in Lotus japonicus. | Shilei Xu Yonghai Luo Zhigang Cai Xiangling Cao Xiaohe Hu Jun Yang Da Luo | 2013 | Journal of Integrative Plant Biology2013,55,3: | 6 |
| 4 | Roles of miR319-regulated TCPs in plant development and response to abiotic stress显示文摘Elaborate regulation of gene expression is required for plants to maintain normal growth,development,and reproduction.MicroRNAs(miRNAs)and transcription factors are key players that control gene expression in plant regulatory networks.The TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR(TCP)family comprises plantspecific transcription factors that contain a conserved TCP domain of 59 amino acids.Some members of this family are targeted by miR319,one of the most ancient and evolutionarily conserved miRNAs in plants.Accumulating evidence has revealed that miR319-regulated TCP(MRTCP)genes participate extensively in plant development and responses to environmental stress.In this review,the structural characteristics and classifications of TCP transcription factors and the regulatory relationships between TCP transcription factors and miRNAs are introduced.Current knowledge of the regulatory functions of MRTCP genes in multiple biological pathways including leaf development,vascular formation,flowering,hormone signaling,and response to environmental stresses such as cold,salt,and drought is summarized.This review will be beneficial for understanding the roles of the MRTCP-mediated regulatory network and its molecular mechanisms in plant development and stress response,and provides a theoretical basis for plant genetic improvement. | Yujie Fang Yuqian Zheng Wei Lu Jian Li Yujing Duan Shuai Zhang Youping Wang | 2021 | The Crop Journal2021,9,1: | 4 |
| 5 | 植物TCP家族基因的研究进展显示文摘TCP家族基因是植物所特有的一类转录因子,参与植物的诸多生长发育过程。本文综述了植物TCP家族基因的结构与分类,并对植物生长发育过程中TCP基因参与的调控过程进行了总结。 | 王景超 | 2021 | 农业与技术2021,41,18: | 3 |
| 6 | 牡丹TCP家族基因的鉴定与生物信息学分析显示文摘TCP(Teosinte branched1/Cincinnata/proliferating cell factor)家族基因是植物特有的一类转录因子,在植物生长发育的过程中具有重要的调控作用。本研究从牡丹转录组数据中鉴定出18个TCP基因,分子量在18978.32~51789.56 Da之间,等电点在5.81~9.45之间,TCP家族成员亚细胞定位预测均在细胞核中。牡丹TCP结构域中保守氨基酸的分类与拟南芥相似,系统发育分析结果表明,牡丹TCP蛋白分为两大类,ClassⅠ包含10个TCP成员,ClassⅡ包含8个TCP成员。MEME分析结果显示,在牡丹TCP蛋白中发现了15个保守结构域,其中motif 1为TCP结构域,在所有TCP成员中都存在。同时,对牡丹TCP基因在芽、叶、花瓣、花斑、雄蕊、雌蕊和种子7个组织中的转录水平进行了分析。本研究对牡丹TCP家族基因进行了系统的分析,为进一步开展牡丹功能基因研究及分子育种提供了科学依据。 | 张延召 罗新 李会云 段旭佳 张妍 | 2022 | 分子植物育种2022,20,1: | 2 |
| 7 | Petal Development in Lotus japonicus显示文摘Previous studies have demonstrated that petal shape and size in legume flowers are determined by two separate mechanisms,dorsoventral (DV) and organ internal (IN) asymmetric mechanisms,respectively.However,little is known about the molecular mechanisms controlling petal development in legumes.To address this question,we investigated petal development along the floral DV axis in Lotus japonicus with respect to cell and developmental biology by comparing wild-type legumes to mutants.Based on morphological markers,the entire course of petal development,from initiation to maturity,was grouped to define 3 phases or 13 stages.In terms of epidermal micromorphology from adaxial surface,mature petals were divided into several distinct domains,and characteristic epidermal cells of each petal differentiated at stage 9,while epidermal cells of all domains were observed until stage 12.TCP and MIXTA-like genes were found to be differentially expressed in various domains of petals at stages 9 and 12.Our results suggest that DV and IN mechanisms interplay at different stages of petal development,and their interaction at the cellular and molecular level guides the elaboration of domains within petals to achieve their ideal shape,and further suggest that TCP genes determine petal identity along the DV axis by regulating MIXTA-like gene expression. | Lin Weng Zhaoxia Tian Xianzhong Feng Xin Li Shilei Xu Xiaohe Hu Da Luo Jun Yang | 2011 | Journal of Integrative Plant Biology2011,53,10: | 1 |
| 8 | Transcriptional and Post-transcriptional Modulation of SQU and KEW Activities in the Control of Dorsal-Ventral Asymmetric Flower Development in Lotus japonicus显示文摘在 Papilionoideae 荚,莲花 japonicus,发展背面腹(DV ) 不对称的花被二 TB1/CYCLOIDEA/PCF (TCP ) 基因,摆平的标准(SQU ) 和翻身翅膀主要在莲花(英国伦敦郊外的村名) 控制,它分别地决定背面、侧面的身份。然而,这二高度相应的基因怎么安排他们的多样的功能的分子的基础仍然保持不清楚。这里,我们分析了他们的表示层次,并且调查了 SQU 和英国伦敦郊外的村名的 transcriptional 活动。我们证明 SQU 拥有激活和压抑活动,当英国伦敦郊外的村名仅仅充当使活跃之物时。他们形成人 -- 并且 heterodimers,然后联合地在抄写水平调整他们的表示。而且,我们识别了 post-transcriptional 修正, phosphorylation 和 ATP/GTP 绑定的二种类型,哪个能影响他们的 transcriptional 活动。在 SQU 和英国伦敦郊外的村名的 ATP/GTP 有约束力的主题的变化导致忍受变异的蛋白质显示器有缺陷者 DV 不对称的花开发的 phosphorylation,和转基因的植物的失败,显示二结合修正为他们的多样的函数是必要的。总的来说, SQU 和英国伦敦郊外的村名活动精确在抄写和抄写以后的层次被调制,它可能连接 DV 不对称的花开发到不同生理的地位或发信号的小径。 | Zhiyong xu Kai Cheng Xin Li Jun Yang Shilei Xu Xiangling Cao Xiaohe Hu Wei Xie Ling Yuan Mike Ambrose Genyun Chen Hualing Mi Da Luo | 2016 | Molecular Plant2016,9,5: | 1 |