| 1 | A pharmacodynamic model of portal hypertension in isolated perfused rat liver显示文摘AIM:To develop a pharmacodynamic model of portal hypertension from chronic hepatitis.METHODS:Pathological changes and collagen depositions were analyzed using morphometry to confirm CCl 4-induced chronic hepatitis.At d 0,d 28,d 56 and d 84 of the process,the portal perfused velocities (μL/min) in isolated rat livers were exactly controlled with a quantified pump.The pressure (mmHg) was monitored with a Physiological System.The geometric concentrations of phenylephrine or acetylcholine were added to a fixed volume (300 mL) of the circulating perfusate.The equation,the median effective concentration and its 95% confidence intervals of phenylephrine or acetylcholine were regressed with Prism-4 software in nonlinear fit and various slopes.In the isolated perfused rat livers with chronic hepatitis,both median effective concentrations were defined as the pharmacodynamic model of portal hypertension.RESULTS:At d 0,d 28,d 56 and d 84,the equations of portal pressure potency from the concentrations of phenylephrine used to constrict the portal vein in isolated perfused rat livers were Y=0.1732 + 0.3970/[1 + 10 (-4.3061-0.4407 X) ],Y=-0.004934 + 0.12113/[1 + 10 (-3.1247-0.3262 X) ],Y=0.0104 + 0.2643/[1 + 10 (-8.8462-0.9579 X) ],and Y=0.01603 + 0.12107/[1 + 10 (-5.1134-0.563 X) ];the median effective concentrations were 1.69 × 10-10 mol/L,2.64 × 10-10 mol/L,5.82 × 10-10 mol/L,and 8.24 × 10-10 mol/L,respectively.The equations from the concentrations of acetylcholine used to relax the portal vein were Y =-0.4548 + 0.3274/[1 + 10 (6.1538 + 0.5554 X) ],Y=-0.05391 + 0.06424/[1 + 10 (3.8541 + 0.3469 X) ],Y=-0.2733 + 0.22978/[1 + 10 (3.0472 + 0.3008 X) ],and Y=-0.0559 + 0.053178/[1 + 10 (5.6336 + 0.5883 X) ];the median effective concentrations were 8.40 × 10-10 mol/L,7.73 × 10-12 mol/L,5.98 × 10-11 mol/L,and 2.66 × 10-10 mol/L,respectively.CONCLUSION:A pharmacodynamic model of portal hypertension in isolated perfused rat livers with chronic hepatitis was defined as the median effective concentrations of phenylephrine and acetylcholine. | Tao Zhang Xue-Yan Xu Hang Zhou Xin Zhao Meng Song Tao-Tao Zhang He Yin Ting Li Peng-Tao Li Da-Yong Cai | 2012 | World Journal of Gastroenterology2012，18，5： | 2 |
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