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| 1 | 附子干姜配伍对阿霉素致大鼠慢性心衰的治疗作用显示文摘目的观察附子干姜配伍治疗阿霉素(ADR)致慢性心衰的作用,并探讨其作用机理。方法采用盐酸ADR腹腔注射方法,建立慢性心力衰竭大鼠模型,将SD大鼠随机分为6组:对照组,模型组,附子高、低剂量组(3.3,1.65 g/kg),附子干姜(1∶1)高、低剂量组(6.7,3.3 g/kg)。采用酶联免疫吸附法(ELISA)检测大鼠血清中去甲肾上腺素(NE)、内皮素-1(ET-1)和心脏组织中Na+-K+-ATPase的活性,运用蛋白免疫印迹杂交法(Western Blot)检测心脏组织蛋白Junctophilin-2(JP2)的蛋白表达。结果与模型组比较,附子和附子干姜配伍血清中NE及ET-1含量显著降低,心脏组织中Na+-K+-ATPase含量及JP2的蛋白表达显著升高,其中附子干姜(1∶1)高剂量组作用更显著(P<0.01)。结论附子与附子干姜(1∶1)配伍对ADR致慢性心衰的作用,可能与其增加心脏组织Na+-K+-ATPase活性,上调Junctophilin-2(JP2)的蛋白表达有关。 | 梁涛 耿珊 周玲玲 葛华雯 王新宇 | 2014 | 中国中医急症2014，23，10： | 13 |
| 2 | 心肌细胞横管的形态发生:Junctophilin-2的作用显示文摘横管系统是心脏和肌肉细胞兴奋收缩偶联的结构基础.在新生鼠的心肌细胞中并不存在横管结构,横管的形态发生是心肌细胞成熟过程中的一个重要标志.本文对心肌细胞成熟过程中横管的形态变化进行定量分析,并对连接横管和肌质网的关键分子Junctophilin-2(JP2)在这个过程中所发挥的作用进行研究.对心肌细胞图像的分析显示,在横管成熟的过程中,横管的横向组分逐渐增加,纵向组分逐渐减少.通过对横管图像进行傅里叶变换分析,发现横管排列的规律性随心肌细胞的成熟而逐渐增加.横管的这些变化伴随着JP2蛋白表达量的上升,并且可以被JP2表达量的敲减所逆转.这些现象表明,JP2在横管形态发生过程中发挥重要作用. | 韩晶 吴昊迪 王其伟 王世强 | 2013 | 中国科学:生命科学2013，43，9： | 5 |
| 3 | Morphogenesis of T-tubules in heart cells: the role of junctophilin-2显示文摘The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not exist in neonatal cardiomyocytes. In the present study, we quantified the morphological changes in TTs during heart cell maturation and investigated the role of junctophilin-2 (JP2), a protein known to anchor the sarcoplasmic reticulum (SR) to TT, in changes to TT morphological parameters. Analysis of confocal images showed that the transverse elements of TTs increased, while longitudinal elements decreased during the maturation of TTs. Fourier transform analysis showed that the power of ~2 m spatial components increased with cardiomyocytes maturation. These changes were preceded by increased expression of JP2, and were reversed by JP2 knockdown. These findings indicate that JP2 is required for the morphogenesis of TTs during heart development. | HAN Jing WU HaoDi WANG QiWei WANG ShiQiang | 2013 | Science China(Life Sciences)2013，56，7： | 4 |
| 4 | Junctophilin-2表达下调介导心肌细胞兴奋—收缩耦联结构与功能的重塑显示文摘目的阐明横管(T-tubule,TT)与肌质网(sarcoplasmic reticulum,SR)耦联关键蛋白junctophilin-2(JP2)的表达下调对心肌细胞二联体超微结构以及兴奋-收缩耦联功能的影响。方法通过构建JP2特异性shRNA腺病毒敲减成年大鼠心肌细胞中JP2的表达后,采用透射电子显微镜观测心肌细胞横管和肌质网二联体结构的形态变化,并进一步采用全细胞膜片钳结合激光共聚焦钙成像技术检测心肌细胞兴奋收缩联联功能的变化。结果通过腺病毒敲减心肌细胞JP2表达后,心肌细胞内总TT数目、TT与SR耦联的二联体结构数目以及单个耦联子内耦联SR的TT长度占TT周长的百分比均显著降低;与此同时,JP2表达下降后心肌细胞钙瞬变幅度降低,收缩功能减弱,表现出与心力衰竭类似的表型。结论心肌细胞JP2表达下降引起心肌细胞兴奋-收缩耦联结构与功能的损伤,为心力衰竭病理情况下心肌细胞结构与功能的调控机制提供了直接有力的实验证据。 | 马元良 黎荣昌 吴昊迪 王世强 徐明 | 2013 | 中国分子心脏病学杂志2013，13，3： | 2 |
| 5 | Differential gene expression profile of Buyanghuanwu decoction in rats with ventricular remodeling post-myocardial infarction显示文摘OBJECTIVE: To investigate the effect of Buyanghuanwu decoction(BYHWD) on gene expression in ventricular remodeling post-myocardial infarction in rats.METHODS: Animal models of myocardial infarction were established by permanent ligation of the left anterior descending coronary artery. Echocardiography measurements were performed after the treatment of BYHWD(18 g·kg-1 collagen was observ·d-1) for 90 days.Myocardialed by mallory trichrome staining. Capillary density was quantified by using Factor rentially expⅧre immunohistochemical staining.Diffessed genes were explored by a short-read sequencing technology combined with a tag-based digital gene expression profiling(DGE)system. Real-time quantitative polymerase chain reaction detecting system(q PCR) was used to validate the sequencing results. After assembling the gene information from Sham, model and BYHWD groups, we constructed three DGE libraries based on each group. The sequencing of three libraries generated 66 000-73 000 unique tags, which were mapped to reference sequences for annotation of expressed genes.RESULTS: Among them, 511 and 352 differentially expressed genes were found in comparison with sham/model and model/BYHWD, respectively. Fifty-five genes exhibited reversed direction of gene expression differences between Sham/Model and Model/BYHWD groups. We found that transforming growth factor beta receptor-1, junctophilin-2,monocyte chemotactic protein 1, neuropeptide Y,arachidonate 5-Lipoxygenase, arachidonate 15-Lipoxygenase were significantly modulated, which suggested the involvement of these genes in BYHWD treatment.CONCLUSION: The DGE profiling data provide comprehensive gene expression information at the transcriptional level that could facilitate our understanding of the pharmacological mechanisms of BYHWD in ventricular remodeling post-myocardial infarction. | Zhang Tao Hua Yue Luo Hao Chen Hongmei Shao Meng Fu Xiuqiong John Man-Tak Chu Huang Guiqiong Liu Bin Zhou Yingchun | 2017 | Journal of Traditional Chinese Medicine2017，37，3： | 1 |
| 6 | 心肌细胞横管-肌质网耦联与钙信号调控显示文摘心肌细胞的兴奋沿横管传入细胞深处并激活L型钙通道,进而通过钙致钙释放机制激活肌质网ryanodine受体钙释放通道,由此产生的钙火花叠加成为细胞钙瞬变,引发心肌细胞同步化收缩.β;型肾上腺素受体介导的广域cAMP信号通过磷酸化L型钙通道、ryanodine受体、肌质网受磷蛋白,分别上调钙内流、钙释放和肌质网钙泵的钙回收速率,从而增强心脏泵血能力.β;型肾上腺素受体一方面通过局域cAMP信号磷酸化L型钙通道,另一方面通过G蛋白耦联受体激酶-2介导的'越位阻隔'抑制β;受体对肌质网钙释放和钙回收的调控.心力衰竭病理过程中,由于铆定横管与肌质网的junctophilin-2下调,横管与肌质网发生结构和功能脱耦联,造成心脏泵血能力低下冬眠动物能通过转录因子myocardin上调junctophilin-2和caveolin-3,增强横管与肌质网的结构和功能耦联,从而保证冬眠状态下的心脏泵血能力.进一步探明横管与肌质网间钙致钙释放稳态调控的分子机制将为有关心脏疾病的防治提供新思路. | 邢昕 梁景辉 王世强 | 2022 | 中国科学:生命科学2022，52，1： | 1 |
| 7 | 大鼠H9c2心肌细胞中SK2通道和JP2蛋白的表达及定位显示文摘目的:研究大鼠H9c2心肌细胞中SK2通道和JP2蛋白的表达及定位,探讨二者的相互作用。方法:采用Western blot检测SK2通道及JP2蛋白在H9c2细胞中的表达,免疫共沉淀鉴定JP2与SK2通道之间的相互作用,免疫荧光标记观察SK2通道和JP2蛋白在H9c2细胞的定位。结果:心肌细胞H9c2表达SK2通道及JP2蛋白,SK2通道和JP2蛋白可形成免疫复合物,SK2通道和JP2蛋白存在部分共定位。结论:心肌细胞H9c2中SK2通道与JP2蛋白存在相互作用。 | 罗天霞 樊红琨 李立人 闫宁宁 章茜 | 2019 | 郑州大学学报(医学版)2019，54，1： | 0 |
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