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| 1 | 茶园土壤性状及茶树营养元素吸收、转运机制研究进展显示文摘优质安全的茶叶离不开养分条件、环境安全和茶园的土、水、肥的管理。本文在茶园土壤、茶树养分功能和分子营养机制、茶树施肥技术、茶园土壤重金属及茶树累积特点和机制等方面,对近五年来国内外的研究进展进行了综述。在茶园土壤研究方面,除了关注植茶后茶园土壤有机质变化特点外,茶园碳储量在全球碳循环中所起的重要作用也引起了人们的重视,同时分子生物学技术也开始应用于茶园土壤微生物种群数量和演变的研究,并在茶园土壤质量评价、土壤酸化原因以及应用生物质改良酸化茶园土壤等方面取得了一定进展。在茶树养分功能和调控技术方面,对主要营养元素氮、磷、钾等的营养功能及其在茶叶品质成分代谢中的作用和茶树吸收特性等有了更深入的认识,在茶树养分转运子基因克隆、氮营养分子生理机制、抗环境胁迫的分子基础等方面的研究逐渐深入,在机械施肥、水肥一体化、控释肥施用等技术研究方面取得较大进展,施肥的环境效应特别是温室气体排放影响成为近年的研究热点。伴随着对食品安全问题的关注,茶叶产地土壤重金属和稀土等的安全状况、茶树累积特点等方面的研究也取得一定进展。 | 刘美雅 伊晓云 石元值 马立锋 阮建云 | 2015 | 茶叶科学2015，35，2： | 50 |
| 2 | Computational Identification of Sweet Wormwood (Artemisia annua) microRNA and Their mRNA Targets显示文摘Despite its efficacy against malaria, the relatively low yield (0.01%-0.8%) of artemisinin in Artemisia annua is a serious limitation to the commercialization of the drug. A better understanding of the biosynthetic pathway of artemisinin and its regulation by both exogenous and endogenous factors is essential to improve artemisinin yield. Increasing evidence has shown that microRNAs (miRNAs) play multiple roles in various biological processes. In this study, we used previously known miRNAs from Arabidopsis and rice against expressed sequence tag (EST) database of A. annua to search for potential miRNAs and their targets in A. annua. A total of six potential miRNAs were predicted, which belong to the miR414 and miR1310 families. Furthermore, eight potential target genes were identified in this species. Among them, seven genes encode proteins that play important roles in ar- temisinin biosynthesis, including HMG-CoA reductase (HMGR), amorpha-4,11-diene synthase (ADS), farnesyl pyrophosphate synthase (FPS) and cytochrome P450. In addition, a gene coding for putative AINTEGUMENTA, which is involved in signal transduction and development, was also predicted as one of the targets. This is the first in silico study to indicate that miRNAs target genes encoding enzymes involved in artemisinin biosynthesis, which may help to understand the miRNA-mediated regulation of artemisinin biosynthesis in A. annua. | Alok Pani Rajani Kanta Mahapatra Niranjan Behera Pradeep Kumar Naik | 2011 | Genomics, Proteomics & Bioinformatics2011，9，6： | 7 |
| 3 | miRNA调控药用植物生长发育和次生代谢显示文摘microRNA(miRNA)作为一类内源性的短链非编码RNA,广泛存在于真核细胞中,主要通过对转录本剪切和抑制翻译等方式,参与转录后基因的表达调控。近年来研究表明,多种药用植物中鉴定出大量的miRNA。这些miRNA对药用植物的生长发育和次生代谢产物合成具有调控功能。次生代谢产物是药用植物的主要有效成分,研究miRNA对药用植物次生代谢过程的调控作用具有十分重要的意义。本文综述了miRNA在植物中的产生途径、作用方式和体内功能,在此基础上重点介绍了miRNA对药用植物生长发育和次生代谢产物生物合成的调控作用,并对药用植物miRNA的研究进行了展望,以期为提高药用植物产量,高效获得药用植物有效成分以及临床应用开拓新的思路。 | 史磊 郭艳兵 申远 | 2019 | 中国生物化学与分子生物学报2019，35，4： | 4 |
| 4 | Identification of miRNAs and target genes regulating catechin biosynthesis in tea (Camellia sinensis)显示文摘Micro RNAs(miRNAs) are endogenous non-protein-coding small RNAs that play crucial and versatile regulatory roles in plants. Using a computational identification method, we identified 55 conserved miRNAs in tea(Camellia sinensis) by aligning miRNA sequences of different plant species with the transcriptome library of tea strain 1005. We then used quantitative real-time PCR(qRT-PCR) to analyze the expression of 31 identified miRNAs in tea leaves of different ages, thereby verifying the existence of these miRNAs and confirming the reliability of the computational identification method. We predicted which miRNAs were involved in catechin synthesis using ps RNAtarget Software based on conserved miRNAs and catechin synthesis pathway-related genes. Then, we used RNA ligase-mediated rapid amplification of c DNA ends(RLM-RACE) to obtain seven miRNAs cleaving eight catechin synthesis pathway-related genes including chalcone synthase(CHS), chalcone isomerase(CHI), dihydroflavonol 4-reductase(DFR), anthocyanidin reductase(ANR), leucoanthocyanidin reductase(LAR), and flavanone 3-hydroxylase(F3H). An expression analysis of miRNAs and target genes revealed that miR529d and miR156 g-3 p were negatively correlated with their targets CHI and F3H, respectively. The expression of other miRNAs was not significantly related to their target genes in the catechin synthesis pathway. The RLM-RACE results suggest that catechin synthesis is regulated by miRNAs that can cleave genes involved in catechin synthesis. | SUN Ping ZHANG Zhen-lu ZHU Qiu-fang ZHANG Guo-ying XIANG Ping LIN Yu-ling LAI Zhong-xiong LIN Jin-ke | 2018 | Journal of Integrative Agriculture2018，17，5： | 3 |
| 5 | Identification of miRNAs and their targets in tea (Camellia sinensis)显示文摘MicroRNAs(miRNAs) are endogenous small RNAs playing a crucial role in plant growth and development,as well as stress responses.Among them,some are highly evolutionally conserved in the plant kingdom,this provide a powerful strategy for identifying miRNAs in a new species.Tea(Camellia sinensis) is one of the most important commercial beverage crops in the world,but only a limited number of miRNAs have been identified.In the present study,a total of 14 new C.sinensis miRNAs were identified by expressed sequence tag(EST) analysis from 47 452 available C.sinensis ESTs.These miRNAs potentially target 51 mRNAs,which can act as transcription factors,and participate in stress response,transmembrane transport,and signal transduction.Analysis of gene ontology(GO),based on these targets,suggested that 37 biological processes were involved,such as oxidation-reduction process,stress response,and transport.Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis inferred that the identified miRNAs took part in 13 metabolic networks.Our study will help further understanding of the essential roles of miRNAs in C.sinensis growth and development,and stress response. | Quan-wu ZHU Yao-ping LUO | 2013 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2013，14，10： | 2 |
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