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| 1 | 转烟酰胺合酶基因甘蓝型油菜盐碱胁迫下叶片蛋白质差异的研究显示文摘盐胁迫是危害农作物的主要非生物胁迫之一.为了能从蛋白质水平揭示盐胁迫下油菜耐盐的分子遗传机制,采用IEF/SDS-PAGE双向凝胶电泳技术对转OsNAS1基因的甘蓝型油菜在碱性盐胁迫下(20mmol/LNa2CO3)的幼叶蛋白进行了分离.通过银染显色,获得了分辨率和重复性较好的双向电泳图谱,PDQuest软件在分子量20~75kD,等电点4~7范围内,发现表达量变化2倍以上的点有12个.对这12个蛋白质点采用MALDI-TOF-MS进行肽质谱指纹图分析,有11个蛋白点得到了可靠的鉴定,其中有5个差异蛋白可能与耐盐性有关,分别是二氢硫辛酸脱氢酶、谷胱甘肽-S-转移酶、过氧化物酶、20S蛋白酶体β亚基以及核酮糖-1,5-二磷酸羧化酶/氧化酶,它们参与了物质能量代谢、蛋白质降解以及细胞防卫等过程,推测转OsNAS1基因的甘蓝型油菜的耐盐性可能与这些生理生化代谢有关.研究结果为揭示转基因油菜耐盐机理提供了理论依据. | 孔芳 毛善婧 杜坤 吴冕 周晓燕 储成才 王幼平 | 2011 | 科学通报2011，56，18： | 2 |
| 2 | Comparative proteomics analysis of OsNAS1 transgenic Brassica napus under salt stress显示文摘Salt stress is one of the major abiotic stresses in agricultural plants worldwide.We used proteomics to analyze the differential expression of proteins in transgenic OsNAS1 and non-transformant Brassica napus treated with 20 mmol/L Na2CO3.Total protein from the leaves was extracted and separated through a high-resolution and highly repetitive two-dimensional electrophoresis(2-DE)technology system.Twelve protein spots were reproducibly observed to be upregulated by more than 2-fold between transgenic and non-transformant B.napus.These 12 spots were digested in-gel with trypsin and characterized by matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)to obtain the peptide mass fingerprints.Protein database searching revealed that 5 of these proteins are involved in salt tolerance:dehydrogenase,glutathione S-transferase,peroxidase,20S proteasome beta subunit,and ribulose-1,5-bisphosphate carboxylase/oxygenase.The potential functions of these identified proteins in substance and energy metabolism,stress tolerance,protein degradation,and cell defense are discussed.The salt tolerance of the transgenic rapeseed was significantly improved by the introduction of the OsNAS1 gene from Brazilian upland rice of Oryza sativa(cv.IAPAR 9). | KONG Fang MAO ShanJing DU Kun WU Mian ZHOU XiaoYan CHU ChengCai WANG YouPing | 2011 | Chinese Science Bulletin2011，56，22： | 2 |
| 3 | 转Bar基因甘蓝型油菜叶片蛋白质组变化的初步分析显示文摘采取SDS-PAGE与MALDI-TOF-MS联用的方法,对抗除草剂转Bar基因T1代甘蓝型油菜与普通栽培油菜的叶片蛋白质组进行比较性研究,获得差异蛋白质组的重要信息,并初步探讨差异蛋白的主要功能,以期找到与转Bar基因油菜抗除草剂有关的蛋白质,揭示其抗性机理。双向电泳表达图谱研究表明,Bar基因的转入使得转基因油菜中的差异蛋白表达质与量发生了显著变化,共得到16个发生差异表达的蛋白质点,其中11个经质谱分析功能得到鉴定,这些鉴定出的蛋白质涉及多个生理过程,如能量与代谢、信号转导、代谢相关蛋白离子转运和防御应答等。 | 孔芳 薛正莲 杨超英 王幼平 | 2012 | 生物技术通报2012，28，10： | 1 |
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