共被期刊论文引用了2次
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| 1 | 病毒诱导的基因沉默及其在植物研究中的应用显示文摘病毒诱导的基因沉默(Virus-induced gene silencing,VIGS)在植物的基因功能鉴定中是一个非常有用的工具,在植物中了得到广泛应用。与传统的研究植物基因阻断的方法相比,VIGS具有简便高效、周期性短、不需要对植物进行转化以及可以沉默基因家族等特点,而且在一定条件下其沉默效应还能够传递给后代。介绍了VIGS的原理与操作过程,综述了该技术的应用与研究成果,有助于推动其在植物尤其是农作物育种中的进一步应用与发展。 | 孙威 许奕 许桂莺 孙佩光 宋顺 常胜合 | 2015 | 生物技术通报2015，31，10： | 5 |
| 2 | Application of virus-induced gene silencing for identification of FHB resistant genes显示文摘Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc. In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)- based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat. With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage. However, the plant may reduce its own immunity to FHB when inoculated with BSMV. To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation. Using this system, TaAOC, TaAOS, and TaOPR3 involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants. | FAN Yan-hui HOU Bing-qian SU Pei-sen WU Hong-yan WANG Gui-ping KONG Ling-rang MA Xin WANG Hong-wei | 2019 | Journal of Integrative Agriculture2019，18，10： | 1 |
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