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| 1 | 植物纤维素合酶显示文摘纤维素合酶与类纤维素合酶构成一个庞大的超基因家族。纤维素合酶基因在植物的不同组织及不同的发育时期,表达种类和表达量都存在差异。不同组织或不同细胞壁发育时期,不同的纤维素合酶与类纤维素合酶参与合成不同类型的纤维素和半纤维素。此外,纤维素合酶在花粉发育过程中行使着重要的功能,但它们确切的功能以及不同的纤维素合酶之间的相互作用关系尚不明确。因此,研究纤维素合酶基因的功能及其作用机制具有非常重要的现实意义。该文介绍了目前这一领域内分类与功能方面的研究进展,为进一步研究纤维素合酶在植物中的作用机制提供新的线索。 | 田爱梅 许丽爱 陶贵荣 于晖 苏丽艳 曹家树 | 2017 | 中国细胞生物学学报2017，39，3： | 4 |
| 2 | The Cooperative Activities of CSLD2, CSLD3, and CSLD5 Are Required for Normal Arabidopsis Development显示文摘Glycosyltransferases of the Cellulose Synthase Like D (CSLD) subfamily have been reported to be involved in tip growth and stem development in Arabidopsis. The csld2 and csld3 mutants are root hair defective and the csld5 mutant has reduced stem growth. In this study, we produced double and triple knockout mutants of CSLD2, CSLD3, and CSLD5. Unlike the single mutants and the csld2/csld3 double mutant, the csld2/csld5, csld3/csld5, and csld2/ csld3/csld5 mutants were dwarfed and showed severely reduced viability. This demonstrates that the cooperative activities of CSLD2, CSLD3, and CSLD5 are required for normal Arabidopsis development, and that they are involved in important processes besides the specialized role in tip growth. The mutant phenotypes indicate that CSLD2 and CSLD3 have overlapping functions with CSLD5 in early plant development, whereas the CSLD2 and CSLD3 proteins are non-redundant. To determine the biochemical function of CSLD proteins, we used transient expression in tobacco leaves. Microsomes containing heterologously expressed CSLD5 transferred mannose from GDP–mannose onto endogenous acceptors. The same activity was detected when CSLD2 and CSLD3 were co-expressed but not when they were expressed separately. With monosaccharides as exogenous acceptors, microsomal preparations from CSLD5-expressing plants mediated the transfer of mannose from GDP–mannose onto mannose. These results were supported by immunodetection studies that showed reduced levels of a mannan epitope in the cell walls of stem interfascicular fibers and xylem vessels of the csld2/csld3/csld5 mutant. | Lan Yin Yves Verhertbruggen Ai Oikawa Chithra Manisseri Bernhard Knierim Lina Prak Jacob Kriiger Jensen Paul Knox Manfred Auer William G.T. Willatsa and Henrik Vibe Scheller | 2011 | Molecular Plant2011，4，6： | 2 |
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