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| 1 | 顶空气相色谱测定灯盏花素提取物中大孔树脂有机溶剂残留物显示文摘目的建立灯盏花素提取物中8种大孔树脂有机溶剂残留物的检测方法。方法采用顶空进样毛细管气相色谱法,色谱柱为HP-INNOWAX毛细管柱(30 m×0.53 mm×1.0μm),柱温起始温度60℃,维持16 min,再以每分钟20℃升温至200℃,维持2 min;进样口温度240℃;FID检测器温度300℃。结果在此条件下,待测物正己烷、苯、甲苯、对二甲苯、邻二甲苯、苯乙烯、12,-二乙基苯、二乙烯苯与内标物正丁醇获得了较好的分离。8种成分在各自的质量浓度范围内呈良好的线性关系(r=0.999,n=7),平均回收率为82.51%~94.92%,RSD为4.9%~6.5%,最低检出限范围为0.02~0.57μg/g。结论该方法简便快速、结果准确、重现性好,可用于灯盏花素提取物中大孔树脂有机溶剂残留量的测定。 | 许勇 郏征伟 钱大公 王柯 季申 | 2011 | 中成药2011，33，8： | 6 |
| 2 | Biodegradation of N,N-dimethylacetamide by Rhodococcus sp.strain B83 isolated from the rhizosphere of pagoda tree显示文摘The biodegradation characteristic and potential metabolic pathway for removal of environmental N,N-dimethylacetamide(DMAC) by Rhodococcus sp. strain B83 was studied.Rhodococcus sp. strain B83 was isolated from the rhizosphere of a pagoda tree and proved capable of utilizing DMAC as sole source of carbon and nitrogen. Batch culture studies showed that strain B83 could tolerate up to 25 g/L DMAC and showed distinct growth on possible catabolic intermediates except for acetate. The nitrogen balance analysis revealed that approximately 71% of the initial nitrogen was converted to organic nitrogen. DMAC degradation has led to accumulation of acetate and organic nitrogen, meanwhile traces of nitrate and ammonia was build-up but without nitrite. The growth of strain B83 could be inhibited by adding exogenous acetate. By means of the assay of enzymatic degradation of DMAC, several catabolic intermediates at different intervals were observed and identified.Based on the results obtained from culture solution and enzymatic degradation assay, a detailed pathway is proposed for DMAC biodegradation. | Xingdu Chen Chengjian Yang Weiwei Wang Bizhou Ge Jun Zhang Yucan Liu Yaping Nan | 2017 | Journal of Environmental Sciences2017，29，3： | 3 |
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