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| 1 | Cytological analysis and genetic control of rice anther development显示文摘Microsporogenesis and male gametogenesis are essential for the alternating life cycle of flowering plants between diploid sporophyte and haploid gametophyte generations.Rice(Oryza saliva) is the world's major staple food,and manipulation of pollen fertility is particularly important for the demands to increase rice grain yield.Towards a better understanding of the mechanisms controlling rice male reproductive development,we describe here the cytological changes of anther development through 14 stages,including cell division,differentiation and degeneration of somatic tissues consisting of four concentric cell layers surrounding and supporting reproductive cells as they form mature pollen grains through meiosis and mitosis.Furthermore,we compare the morphological difference of anthers and pollen grains in both monocot rice and eudicot Arabidopsis thaliana.Additionally,we describe the key genes identified to date critical for rice anther development and pollen formation. | Dabing Zhang Xue Luo Lu Zhu | 2011 | Journal of Genetics and Genomics2011，38，9： | 56 |
| 2 | Two Tightly Linked Genes at the hsal Locus Cause Both F1 and F2 Hybrid Sterility in Rice显示文摘与绝育(F 2 绝育) 联系的混合故障的分子的机制糟糕 F 1 混血儿绝育作为与那些相比被理解。以前,我们描绘了三解开的 epistatic loci,混合 sterility-a1 (hsa1 ) , hsa2,和 hsa3,为在在 Oryza sativa ssp 之间的一个十字的 F 2 绝育负责。indica 和装饰用的梨树。在这研究,我们鉴别 hsa1 地点包含二交往的基因, HSA1a 和 HSA1b,在一个 30-kb 区域以内。HSA1a-j (装饰用的梨树等位基因) 编码未知函数蛋白质(DUF1618 ) 的高度保存的植物特定的域, whereasthe indica 等位基因(HSA1a -- i s ) 有引起域结构的混乱的二个删除变化。第二基因, HSA1b -- i s , 编码 uncharacterized proteinwith 某类似到核苷酸绑定蛋白质。indica HSA1a 的同型结合的基因渗入 -- i s -HSA1b-i 进装饰用的梨树的 s 等位基因在一个早有丝分裂的阶段显示出女配偶子流产。事实 recombinant haplotype HSA1a-j-HSA1b-i s 与 HSA1a 处于异质接合的状态引起了半绝育 -- i s -HSA1b-i s haplotype 建议在 hsa1 地点的那个变化是在米饭在 F 1 混血儿和连续的代看见的宽光谱的绝育障碍的一个可能的原因。我们建议一个简单基因模型解释单个原因的机制怎么能驾驶 F 1 和 F 2 混血儿绝育。 | Takahiko Kubo | 2016 | Molecular Plant2016，9，2： | 14 |
| 3 | 水稻籼粳杂种不育与广亲和显示文摘水稻是世界主要粮食作物,水稻杂种优势的利用为提高作物产量做出了巨大贡献.亚洲栽培稻分为籼稻和粳稻两个亚种,亚种间的杂种优势更强,培育籼粳杂交水稻可以进一步提高水稻产量潜力.但是亚种间普遍存在生殖隔离,导致籼粳杂种育性较低,限制了对其杂种优势的利用.广亲和品种与籼稻和粳稻的杂交后代正常可育,为利用亚种间杂种优势提供重要种质资源.本文回顾了籼粳杂交育种的历史,总结了水稻杂种不育的遗传基础和分子调控机理的研究进展,介绍了广亲和基因形成机制的最新理解,在此基础上概述了近年来对广亲和基因发掘和利用的成果,并展望了未来创建广亲和水稻种质资源的途径. | 欧阳亦聃 | 2016 | 科学通报2016，61，35： | 14 |
| 4 | 基于红外热成像技术和广义回归神经网络的稻种发芽率检测方法研究(英文)显示文摘基于稻种老化时间不同时的物理学和生理学差异,提出一种基于红外热成像技术及广义回归神经网络的快速、无损检测稻种发芽率的检测方法,解决传统稻种发芽率检测方法操作复杂、实验周期长等问题。在温度为45℃、湿度为90%的条件下,将水稻种子依次老化0,1,2,3,4,5,6和7 d,得到不同发芽率的种子;采集稻种红外热图像,然后提取稻种胚芽部位数据,总计144份,随机分为校正集和预测集,其中校正集96份,预测集48份;分析和比较不同老化天数稻种红外热差异,从物理学和生理学方面揭示稻种发芽率与红外热图像间的关系,结合偏最小二乘算法(partial least squares,PLS)、BP(back propagation,BP)人工神经网络和广义回归神经网络(general regression neural network,GRNN),建立稻种发芽率的红外热模型。结果表明,利用GRNN建立的发芽率预测模型效果最优,其中校正集的Rc(相关系数)和SEC(标准偏差)分别为0.932 0和2.056 0,预测集RP(相关系数)和SEP(标准偏差)分别为0.900 3和4.101 2,相关性均达到较高水平且校正集与预测集的标准偏差均较小。实验结果表明,采用红外热成像技术结合广义回归神经网络研究稻种发芽率是可行的,且所建模型在稻种发芽率快速测定方面有较高的精度。 | 方文辉 卢伟 徐鸿力 洪德林 梁琨 | 2016 | 光谱学与光谱分析2016，36，8： | 9 |
| 5 | The molecular and evolutionary basis of reproductive isolation in plants显示文摘Reproductive isolation is defined as processes that prevent individuals of different populations from mating, survival or producing fertile offspring. Reproductive isolation is critical for driving speciation and maintaining species identity, which has been a fundamental concern in evolutionary biology. In plants,reproductive isolation can be divided into prezygotic and postzygotic reproductive barriers, according to its occurrence at different developmental stages. Postzygotic reproductive isolation caused by reduced fitness in hybrids is frequently observed in plants, which hinders gene flow between divergent populations and has substantial effects on genetic differentiation and speciation, and thus is a major obstacle for utilization of heterosis in hybrid crops. During the past decade, China has made tremendous progress in molecular and evolutionary basis of prezygotic and postzygotic reproductive barriers in plants. Present understandings in reproductive isolation especially with new data in the last several years well support three evolutionary genetic models, which represent a general mechanism underlying genomic differentiation and speciation. The updated understanding will offer new approaches for the development of wide-compatibility or neutral varieties, which facilitate breeding of hybrid rice as well as other hybrid crops. | Yidan Ouyang Qifa Zhang | 2018 | Journal of Genetics and Genomics2018，45，11： | 9 |
| 6 | Genetic and Physiological Analysis of a Novel Type of Interspecific Hybrid Weakness in Rice显示文摘混合软弱是在 post-zygotic 阶段妨碍在不同种类之间的基因交换的一个重要繁殖障碍。然而,我们位于混合软弱下面的分子的机制的理解是有限的。在这研究,我们在染色体片断替换线(CSSL ) 图书馆从在 indica 变化 Teqing (Oryza sativa ) 之间的一个十字导出的米饭和普通野米饭报导新奇种间的混合软弱的发现(O。rufipogon ) 。从野米饭的主导的混合软弱 i1 (Hwi1 ) 基因与 Teqing 遗传上不兼容并且导致一套软弱症状,包括王冠根开始的生长抑制,收益减少,损害滋养的吸收,和延迟。植物激素处理证明水杨酸酸(SA ) 能恢复表示混合软弱的植物的高度,当另外的植物激素看起来有小效果时。好印射显示 Hwi1 位于一辆双人脚踏车充满白氨酸的重复像受体的 kinase (LRRRLK ) 基因簇。在染色体 11 的短手臂上的 13.2-kb 候选人区域以内,有二注解 LRRRLK 基因, LOC_Os11g07230 和 LOC_Os11g07240。LOC_Os11g07230 的 Teqing 等位基因和 LOC_Os11g07240 的野米饭等位基因编码预言的功能的蛋白质。基于混合软弱的基因继承, LOC_Os11g07240 为 Hwi1 作为候选人基因被含有。Hwi1 的功能的分析将在米饭扩展我们混合软弱的规定的知识。 | Chen Chen Hao Chen Jun-Xiang Shan Mei-Zhen Zhu Min Shi Ji-Ping Gao Hong-Xuan Lin | 2013 | Molecular Plant2013，6，3： | 6 |
| 7 | Genome-wide dissection of segregation distortion using multiple inter-subspecific crosses in rice显示文摘Mendelian inheritance can ensure equal segregation of alleles from parents to offspring, which provides fundamental basis for genetics and molecular biology. Segregation distortion(SD) leads to preferential transmission of certain alleles from generation to generation. Such violation of Mendelian genetic principle is often accompanied by reproductive isolation and eventually speciation. Although SD is observed in a wide range of species from plants to animals, genome-wide dissection of such biased transmission of gametes is rare. Using nine inter-subspecific rice crosses, a genome-wide screen for SD loci is performed, which reveals 61 single-locus quantitative trait loci and 194 digenic interactions showing distorted transmission ratio, among which 24 new SD loci are identified. Biased transmission of alleles is observed in all nine crosses, suggesting that SD exists extensively in rice populations. 72.13% distorted regions are repeatedly detected in multiple populations, and the most prevalent SD hotspot that observed in eight populations is mapped to chromosome 3. Xian alleles are transmitted at higher frequencies than geng alleles in inter-subspecific crosses, which change the genetic composition of the rice populations. Epistatic interaction contributes significantly to the deviation of Mendelian segregation at the whole-genome level in rice, which is distinct from that in animals. These results provide an extensive archive for investigating the genetic basis of SD in rice, which have significant implications in understanding the reproductive isolation and formation of inter-subspecific barriers during the evolution. | Guangwei Li Jiye Jin Yan Zhou Xufeng Bai Donghai Mao Cong Tan Gongwei Wang Yidan Ouyang | 2019 | Science China(Life Sciences)2019，62，4： | 4 |
| 8 | Deciphering Starch Quality of Rice Kernels Using Metabolite Profiling and Pedigree Network Analysis显示文摘米饭谷物的生理的性质对消费者立即明显。米饭差异研究集合的高范围的 metabolomic 描述预言了在丰满的酸和类脂化合物之间的否定关联层次和直链淀粉 / 总数淀粉比率(直链淀粉比率) ,而是为这的原因是不清楚的。为了在米饭核,淀粉小粒结构,直链淀粉比率,和代谢物的视觉显型之中获得新卓见进关系,变化,我们与各种各样的直链淀粉比率和二猛烈异种调查了五装饰用的梨树栽培变种的代谢物变化( e1 ,淀粉 synthase IIIa ( SSIIIa )缺乏的异种和 SSIIIa/starch 分叉的酶()双大美人异种 4019 )由使用集体 基于spectrometry 的 metabolomics 技术。扫描电子显微镜学清楚地证明二异种有不平常的淀粉小粒结构。,有高直链淀粉比率(Hoshiyutaka 和 Yumetoiro ) 的二栽培变种的 metabolomic 作文展出了类似的模式双大美人异种,它有极其高的直链淀粉比率,不同。栽培变种和异种的瑞斯家谱网络分析在在日本繁殖的米饭提供了卓见进在新陈代谢特点的性质和他们的内在的基因基础之间的协会。多维的可伸缩分析表明 Hoshiyutaka 和 Yumetoiro 栽培变种是象 Indica 一样,然而,他们作为装饰用的梨树 subpopulations 被分类。探索 metabolomic 特点是跟随米饭的一个强大的方法基因踪迹和繁殖历史。 | Miyako Kusano Atsushi Fukushima Naoko Fujita Yozo Okazaki Makoto Kobayashi Naoko Fujita Oitome Kaworu Ebana Kazuki Saito | 2012 | Molecular Plant2012，5，2： | 4 |
| 9 | Sterility and fertility:Keys for crop production in China显示文摘Agricultural production is among the most important issues in China, since the country possesses a population exceeding 1.3 billion people. The arable land per capita, however, comprises only about 1/3 of the world average and continues to decrease owing to rapid urbanization. | Sodmergen | 2010 | Science China(Life Sciences)2010，53，12： | 1 |
| 10 | Exploring the genetic characteristics of 93-11 and Nipponbare recombination inbred lines based on the Golden Gate SNP assay显示文摘Understanding genetic characteristics in rice populations will facilitate exploring evolutionary mechanisms and gene cloning. Numerous molecular markers have been utilized in linkage map construction and quantitative trait locus(QTL) mappings. However, segregation-distorted markers were rarely considered, which prevented understanding genetic characteristics in many populations. In this study, we designed a 384-marker Golden Gate SNP array to genotype 283 recombination inbred lines(RILs) derived from 93-11 and Nipponbare Oryza sativa crosses. Using 294 markers that were highly polymorphic between parents, a linkage map with a total genetic distance of 1,583.2 c M was constructed, including 231 segregation-distorted markers. This linkage map was consistent with maps generated by other methods in previous studies. In total, 85 significant quantitative trait loci(QTLs) with phenotypic variation explained(PVE) values?5% were identified. Among them, 34 QTLs were overlapped with reported genes/QTLs relevant to corresponding traits, and 17 QTLs were overlapped with reported sterility-related genes/QTLs. Our study provides evidence that segregation-distorted markers can be used in linkage map construction and QTL mapping. Moreover, genetic information resulting from this study will help us to understand recombination events and segregation distortion. Furthermore, this study will facilitate gene cloning and understanding mechanism of inter-subspecies hybrid sterility and correlations with important agronomic traits in rice. | Renbo Yu Wei Yan Manzhong Liang Xiaojun Dai Haodong Chen Yunong Sun Xingwang Deng Xiangding Chen Hang He Liangbi Chen | 2016 | Science China(Life Sciences)2016，59，7： | 1 |
| 11 | 水稻S5-ORF3蛋白质的进化分析显示文摘生殖隔离在形成新物种和物种同一性的保持中有重要作用,因而在水稻优化育种中有重要的意义。S5位点是一个已克隆的控制水稻生殖隔离的基因,它产生的S5-ORF3,ORF4和ORF5蛋白质共同调控着indica-japonica杂交后代的育性,特别是S5-ORF3蛋白质在打破水稻亚种indica-japonica之间的生殖隔离与促进物种间的基因交流中发挥重要作用。针对S5-ORF3的进化分析对于研究它的功能和起源很重要,但目前尚无报道。本文基于序列和进化分析,提出S5-ORF3为一种定位在内质网中的新的HSP70家族蛋白,但缺乏HSP70的C端的多肽结合结构域,推测S5-ORF3可能通过影响alpha-淀粉酶的合成来作用于内质网压力;找到了ORF3的19条同源蛋白,并用极大似然算法构建了可靠的进化树,发现水稻的S5-ORF3与节节麦的luminal-binding蛋白进化关系最为密切;作了置信度100%的S5-ORF3蛋白质的三维结构预测,预测了居中的配体绑定位点;发现了水稻的S5-ORF3与其他HSP70蛋白相比独特的基序,为进一步研究S5-ORF3的作用机理和演化历史提供了线索和数据支持。 | 张达瀚 张屹 | 2014 | 河北科技大学学报2014，35，4： | 1 |
| 12 | 物种形成基因及其功能显示文摘什么是物种?新物种是如何形成的?这些问题是生命科学研究的重大问题.物种的形成是在生殖隔离的基础上某些新的生物学性状的形成和保留,是生物进化的最基本过程,其实质是基因结构突变的积累与功能的分化.地理隔离使群体中的基因不能交流,基因突变也会影响个体间交配趣向,从而造成交配隔离或者交配后杂合体的基因组不亲和、杂交不育甚至杂交不活,使不同的群体逐渐分化为新物种.随着分子生物学与基因组学的飞速发展,进化生物学家已经发现一些与物种形成有关的基因—物种形成基因(speciation genes),鉴定并了解这些基因的功能,不仅能使我们在分子水平上理解新物种形成的实质和规律、而且对于我们突破种间屏障进行远缘杂交育种也有重要的理论指导意义.本文综述了目前对几个物种形成基因及其功能的研究进展,为该领域的进一步研究提供资料. | 邢光磊 王昆 王标 邢万金 | 2011 | 中国生物化学与分子生物学报2011，27，1： | 0 |
| 13 | 水稻籼粳亚种间组合合子前生殖隔离及其与杂种育性的关系显示文摘生殖隔离对新物种形成和维系至关重要,其作用机制是生命科学的核心科学问题之一.水稻籼粳亚种间存在生殖隔离现象.迄今为止,对水稻籼粳亚种间合子前生殖隔离机制的研究还不够深入.本研究选用籼稻、粳稻和广亲和品种在内的12个品种,进行亚种间、亚种内和带广亲和基因的亚种间17个组合的杂交实验.通过扫描电子显微镜观察花粉在柱头上的附着,发现2/3的亚种间杂交组合柱头平均附着花粉粒数不足5粒,存在较强的合子前生殖隔离现象;3/4的亚种内杂交组合附着花粉粒数多于5粒;所有的带广亲和基因的亚种间杂交组合附着花粉粒数均大于5粒.同时观察到亚种间杂交花粉管伸长多出现空瘪、盘绕等异常现象,亚种内及带广亲和基因的亚种间组合比较正常.研究还发现,杂交组合花粉附着量与其F1育性不相关,说明合子前与合子后生殖隔离是两个独立的事件.水稻籼粳亚种间通过花粉粒亲和性和萌发状态的差异阻碍杂种后代的产生,该现象是合子前生殖隔离的表现形式.研究结果有助于进一步理解生殖隔离的机制,同时对杂种优势的利用具有一定启发意义. | 牛双双 于义溟 徐才国 李广伟 欧阳亦聃 | 2014 | 中国科学:生命科学2014，44，8： | 0 |
| 14 | A hybrid sterile locus leads to the linkage drag of interspecific hybrid progenies显示文摘Interspecific hybridization plays an important role in rice breeding by broadening access to desirable traits such as disease resistance and improving yields.However,interspecific hybridization is often hindered by hybrid sterility,linkage drag,and distorted segregation.To mine for favorable genes from Oryza glaberrima,we cultivated a series of BC4 introgression lines(ILs)of O.glaberrima in the japonica rice variety background(Dianjingyou 1)in which the IL-2769(BC4F10)showed longer sterile lemmas,wider grains and spreading panicles compared with its receptor parent,suggesting that linkage drag may have occurred.Based on the BC5F2 population,a hybrid sterility locus,S20,a long sterile lemma locus,G1-g,and a new grain width quantitative trait locus(QTL),qGW7,were mapped in the linkage region about 15 centimorgan(cM)from the end of the short arm of chromosome 7.The hybrid sterility locus S20 from O.glaberrima eliminated male gametes of Oryza sativa,and male gametes carrying the alleles of O.sativa in the heterozygotes were aborted completely.In addition,the homozygotes presented a genotype of O.glaberrima,and homozygous O.sativa were not produced.Surprisingly,the linked traits G1-g and qGW7 showed similar segregation distortion.These results indicate that S20 was responsible for the linkage drag.As a large number of detected hybrid sterility loci are widely distributed on rice chromosomes,we suggest that hybrid sterility loci are the critical factors for the linkage drag in interspecific and subspecific hybridization of rice. | Mianmian Wang Jun Yang Jinpeng Wan Dayun Tao Jiawu Zhou Diqiu Yu Peng Xu | 2020 | Plant Diversity2020，42，5： | 0 |
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