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| 1 | Molecular characteristics of three thymosin-repeat proteins from Marsupenaeus japonicus and their responses to WSSV infection显示文摘β-thymosins, a family of highly conserved peptides, play a vital role in wound-healing, angiogenesis,antimicrobial process and antiviral immunity. Three novel β-thymosin-repeat proteins, named mjthm4, mjthm3 and mjthm2, were cloned from Marsupenaeus japonicus using expressed sequence tags(EST) from suppression subtractive hybridization. The mjthm4, mjthm3 and mjthm2 c DNAs possessed open reading frames that encoded166, 128 and 90 amino acid residue polypeptides and contained four, three and two β-thymosin actin binding modules, respectively. Blast analysis demonstrated that mjthm4, mjthm3 and mjthm2 shared high homology with known invertebrate multi-repeat β-thymosins. These proteins are ubiquitously expressed in all of the examined tissues, and the transcriptional levels were highest in the intestine. Further investigation revealed that mjthm4,mjthm3 and mjthm2 were remarkably up-regulated 6 h after WSSV infection. Moreover, while mjthm4 transcriptional levels displayed no changes, mjthm3 and mjthm2 levels decreased in the virus-resistant shrimps.The results indicate that mjthm4, mjthm3 and mjthm2 are novel multi-repeat β-thymosin homologues, have a close relationship with WSSV infection, and might contribute to a better understanding of host defense and/or virus invasion interactions in shrimps. | MA Jinyou RUAN Lingwei XU Xun GAO Zhaoming | 2016 | Acta Oceanologica Sinica2016，35，4： | 5 |
| 2 | 利用多重PCR同时检测WSSV和MBV两种对虾病毒的研究显示文摘研究了检测斑节对虾(Penaeus monodon)的主要致病病原--对虾白斑综合征病毒(white spot syndrome virus,WSSV)及斑节对虾杆状病毒(monodon baculovirus,MBV)的技术.用多重PCR检测方法,设计了两对特异性引物,从不同虾池中收集斑节对虾,提取DNA模板,同时检测两种对虾病毒.研究结果表明:该方法检测灵敏度高、特异性好,可检测至每毫克组织100个病毒粒子;从对虾组织中提取的DNA模板对病毒DNA的扩增无抑制,适合于对虾中两种病毒的同时检测. | 徐丽美 杨丰 | 2005 | 高技术通讯2005，15，5： | 4 |
| 3 | 双重PCR同时检测对虾白斑综合征病毒(WSSV)和传染性皮下及造血器官坏死病毒(IHHNV)显示文摘根据Genbank中对虾白斑病由白斑综合征病毒(WSSV)和传染性皮下及造血器官坏死病毒(IHHNV)的基因序列,设计了两对能分别检测WSSV和IHHNV保守片段基因的特异性引物,而且这两对引物在同一反应体系中可以同时对WSSV和IHHNV的DNA模板进行多重PCR扩增,得到大小分别为110bp(WSSV)和356bp(IHHNV)的扩增条带。对影响PCR反应的主要因素Mg“浓度和退火温度进行了优化,证明当Mg^2+浓度为2.0~4.0mmd,退火温度为57~59℃时可获得最佳的扩增和检测效果。特异性试验结果表明,这两对引物检测WSSV和IHHNV具有很好的特异性,对其它对虾常见病原的PCR扩增结果均为阴性。敏感性测定结果表明,该反应体系最低能检测100pg的WSSV和IHHNV的DNA模板。临床检测表明,所建立的双重PCR方法可以适用于WSSV和IHHNV的同时检测和鉴别。 | 任聪 龚艳清 陈信忠 徐淑菲 王寿昆 | 2008 | 福建水产2008，30，4： | 3 |
| 4 | Expression profiles of penaeidin from Fenneropenaeus chinensis in response to WSSV and vibrio infection by real-time PCR显示文摘Penaeidin from Chinese shrimp (Fenneropenaeus chinensis) has proved to be one of the most important antimicrobial peptides in the bodies of animals. The relative quantitative real-time PCR method is developed to study through time, the mRNA expression profile of penaeidin in the muscle and haemocyte tissue of Chinese shrimp infected with vibrio (Vibrio anguillarum) and WSSV (white spot syndrome virus). Research results showed that the same pathogens infection experiments produced similar gene expression profile in different tissues while different expression profiles appeared in the same tissues infected by different exterior pathogens. In vibrio infection experiments, a 'U' like expression profile resulted. Expression levels of penaeidin increased and surpassed the non-stimulated level, indicating that penaeidin from Chinese shrimp has noticeable antimicrobial activities. In WSSV infection experiments, the expression profile appeared as an inverse 'U' with the expression of penaeidin gradually decreasing to below baseline level after 24 h. The expression of antimicrobial peptides gene in mRNA level in response to virus infection in shrimp showed that international mechanisms of virus to haemocytes and microbial to haemocytes are completely different. Decline of penaeidins expression levels may be due to haemocytes being destroyed by WSSV or that the virus can inhibit the expression of penaeidins by yet undiscovered modes. The expression profiles of penaeidin in response to exterior pathogen and the difference of expression profiles between vibrio and WSSV infection provided some clues to further understanding the complex innate immune mechanism in shrimp. | DONGBo LIUFengsong XIANGJianhai LIFuhua GAOHongwei | 2005 | Acta Oceanologica Sinica2005，24，2： | 3 |
| 5 | 定量PCR法判定对虾白斑杆状病毒早期的感染和增殖显示文摘对虾白斑杆状病毒又称白斑综合症病毒,是对虾养殖业危害最严重的病原体,至今未找到有效的防治方法.充分了解病毒的分子生物学特性和分子致病机理,是病害防治的根本途径,了解病毒的动态增殖特征是该研究的基础.本文采用定量PCR技术,研究对虾白斑杆状病毒人工注射感染后,早期的增殖规律以及感染致死对虾的病毒累积.并对对虾感染病毒后存活时间与个体大小的关系进行了观察.研究发现初期感染病毒含量有短期下降,然后才呈现递增的过程.死亡对虾病毒累积量大于1011病毒粒子/毫克组织(P<0.01).而在4.6~11.6g范围内,感染对虾存活时间与对虾质量不存在相关性(P>0.2). | 兰永胜 章晓波 徐洵 | 2003 | 厦门大学学报(自然科学版)2003，42，6： | 3 |
| 6 | 一步二温式RT-PCR检测南美白对虾桃拉综合症病毒显示文摘建立一种一步法和二温式的逆转录聚合酶链式反应,用于扩增南美白对虾桃拉综合症病毒231bp的特异基因片断,对300份南美白对虾临床样品的检测结果表明,这是一种快速和敏感的桃拉综合症病毒诊断方法。 | 陈晓汉 曾地刚 彭敏 雷爱莹 李咏梅 蒋伟明 | 2005 | 西南农业学报2005，18，3： | 2 |
| 7 | 应用AllGlo探针检测对虾白斑综合征病毒研究显示文摘用AllGlo探针检测对虾白斑综合征病毒,阳性反应呈典型的S曲线,说明本检测探针和引物设计合理。经灵敏度测定,当样品DNA浓度低至100拷贝/μL时,仍可以有效扩增,但在103/μL之上时结果最佳。同时,经过与传统探针检测对比试验得出:用Taqman标记的探针检测相同量的样品时,AllGlo MAR标记的探针(FAM通道)CT值与FAM标记的探针的CT值没有明显区别,但是MAR标记的探针的荧光信号强度要比FAM标记的探针约高40%,更适合于对虾白斑综合征病毒的检测。 | 陈定虎 杨雷亮 冯云 张建威 潘星 徐守振 | 2010 | 中国动物检疫2010，27，11： | 1 |
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