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| 1 | Cloning,expression and characterization of a nucleoside diphosphate kinase (NDPK) gene from tobacco显示文摘Nucleoside diphosphate kinase (NDPK ) 是维持除了 ATP,在生合成使用的所有(d) NTP 的细胞内部的层次的家务酶。这里,我们报导编码 nucleoside diphosphate kinase (NDPK ) 的全身的 cDNA 用酵母被克隆二混血儿的途径。烟草 NDPK 基因被获得并且指定了为 NtNDPK1。NtNDPK1 是在长度的 704 bp 并且编码一通常认为 148 氨基酸的 16.2 kD 蛋白质。系统发生的分析证明 NtNDPK1 对另外的植物 NDPK 基因高度相应并且作为类型 i (NDPK1 ) 识别。RNA 胶化污点分析证明没有在根的 NtNDPK1 表示的重要差别,起源,离开并且发芽。并且 NtNSPK1 的表示被骆驼毛的织物和木钉导致并且由 NaCl 镇压了,然而并非显著地由 Paraquat 影响了,伤害并且低温度(4 ° C ) 处理, NtNDPK1 可以玩的指示响应不能生活的应力的某个角色。在 vitrophosphorylation,试金证明 NtNDPK1 举办了 autophosphorylation 活动。 | Zhou Qiyun Xie Zongming Zhang Zhigang Cao Yangrong Zhang jinsong Chen Shouyi | 2007 | Progress in Natural Science:Materials International2007，17，8： | 4 |
| 2 | Characterization of an ethylene receptor homolog gene from rice显示文摘Ethylene plays important roles in plant growth, development and stress responses. Its receptor genes have been studied in dicots such as Arabidopsis, tobacco and tomato. However, no research has been reported for the ethylene receptors from monocots currently. In the present study, we cloned an ethylene receptor gene OSPK2 from rice and found that its encoded protein was divergent from the ethylene receptors from dicots. OSPK2 had a long extension in its N- terminal, followed by three transmembrane segments, a GAF domain, a putative kinase domain and a putative receiver domain. Although most of the domains were conserved, the expected phosphorylation site His and the phosphate receiver Asp have been replaced by Gln and Asn, respectively. This fact indicates that OSPK2 may not function as a histidine kinase in a phosphorelay manner, but rather play roles by other mechanism, probably through Ser/Thr kinase activity. The expression of the OSPK2 gene was investigated by RT-PCR method under different conditions. We found that this gene was apparently induced by wounding and PEG treatment, but not significantly affected by salt and ABA treatments. The differential expression of the OSPK2 gene may reflect its roles in mediating different abiotic stress responses, consistent with our previous studies on tobacco ethylene receptors. | 曹宛虹 董亿 张劲松 陈受宜 | 2003 | Science China(Life Sciences)2003，46，4： | 2 |
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