共被期刊论文引用了3次
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| 1 | Purification and characterization of a novel endo-β-1,4-glucanase, AfEG22, from the giant snail, Achatina fulica frussac显示文摘在这研究,我们证实了那至少三 endo -- 1,4-glucanases 在巨大的蜗牛的消化果汁存在, Achatina fulica ferussac,由刚果红染色试金。这些酶之一,新奇 endo -- 1,4-glucanase (AfEG22 ) ,是由胶化过滤,阴离子交换,和恐水病的相互作用层析的净化的 29.5 褶层。净化的酶的 carboxymethyl 纤维素(CMC ) hydrolytic 活动是 12.3 U/mg 蛋白质。AfEG22 的分子的团是 MALDI-TOF 决定的 22081 Da。N 终端氨基酸定序揭示了 EQRCTNQGGILKYYNT 的一个序列,它没在强风数据库与任何蛋白质有重要相同。为向 CMC 的 hydrolytic 活动的最佳的 pH 和温度是 pH 4.0 和 | Yigang Teng Qiuyu Yin Ming ding Fukun Zhao | 2010 | Acta Biochimica et Biophysica Sinica2010，42，10： | 3 |
| 2 | 多功能纤维素酶(EGXA)基因在福寿螺胃、肠和肝脏表达水平的测定显示文摘为了探明多功能纤维素酶在不同消化器官的表达情况,采用RT-PCR方法测定了多功能纤维素酶在野生福寿螺胃、肠和肝脏3种组织中的相对表达量。结果表明:EGXA基因在胃、肠和肝脏的表达水平(2-△△Ct)依次为:(1.56±0.13)、(0.08±0.01)、(0.12±0.03),即多功能纤维素酶主要由福寿螺胃分泌。 | 程天印 李姣 | 2013 | 中国农学通报2013，29，35： | 2 |
| 3 | Dynamic changes in the date palm fruit proteome during development and ripening显示文摘Date palm(Phoenix dactylifera)is an economically important fruit tree in the Middle East and North Africa and is characterized by large cultivar diversity,making it a good model for studies on fruit development and other important traits.Here in gel comparative proteomics combined with tandem mass spectrometry were used to study date fruit development and ripening.Total proteins were extracted using a phenol-based protocol.A total of 189 protein spots were differentially regulated(pf0.05).The identified proteins were classified into 14 functional categories.The categories with the most proteins were‘disease and defense’(16.5%)and‘metabolism’(15.4%).Twenty-nine proteins have not previously been identified in other fleshy fruits and 64 showed contrasting expression patterns in other fruits.Abundance of most proteins with a role in abiotic stress responses increased during ripening with the exception of heat shock proteins.Proteins with a role in anthocyanin biosynthesis,glycolysis,tricarboxylic acid cycle and cell wall degradation were upregulated particularly from the onset of ripening and during ripening.In contrast,expression of pentose phosphate-and photosynthesis-related proteins decreased during fruit maturation.Although date palm is considered a climacteric species,the analysis revealed downregulation of two enzymes involved in ethylene biosynthesis,suggesting an ethylene-independent ripening of‘Barhi’fruits.In summary,this proteomics study provides insights into physiological processes during date fruit development and ripening at the systems level and offers a reference proteome for the study of regulatory mechanisms that can inform molecular and biotechnological approaches to further improvements of horticultural traits including fruit quality and yield. | Claudius Marondedze Christoph Gehring Ludivine Thomas | 2014 | Horticulture Research2014，1，1： | 1 |
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