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| 1 | Astragalus polysaccharides can regulate cytokine and P-glycoprotein expression in H22 tumor-bearing mice显示文摘AIM:To investigate the adjunct anticancer effect of Astragalus polysaccharides in H22 tumor-bearing mice.METHODS:To establish a solid tumor model,5.0 × 10 6 /mL H22 hepatoma cells were inoculated subcutaneously into the right armpit region of Kunming mice(6-12 wk old,18-22 g).When the tumors reached a size of 100 mm 3,the animals were treated as indicated,and the mice were randomly assigned to seven groups(n = 10 each).After ten days of treatment,blood samples were collected from mouse eyes,and serum was harvested by centrifugation.Mice were sacrificed,and the whole body,tumor,spleen and thymus were weighed immediately.The rate of tumor inhibition and organ indexes were calculated.The expression levels of serum cytokines,P-glycoprotein(P-GP) and multidrug resistance(MDR) 1 mRNA in tumor tissues were detected using enzyme-linked immunosorbent assay,Western blotting,and quantitative myeloid-derived suppressor cells reverse transcription-polymerase chain reaction,respectively.RESULTS:The tumor inhibition rates in the treatment groups of Adriamycin(ADM) + Astragalus polysaccharides(APS)(50 mg/kg),ADM + APS(100 mg/kg),and ADM + APS(200 mg/kg) were significantly higher than in the ADM group(72.88% vs 60.36%,P = 0.013;73.40% vs 60.36%,P = 0.010;77.57% vs 60.36%,P = 0.001).The spleen indexes of the above groups were also significantly higher than in the ADM group(0.65 ± 0.22 vs 0.39 ± 0.17,P = 0.023;0.62 ± 0.34 vs 0.39 ± 0.17,P = 0.022;0.67 ± 0.20 vs 0.39 ± 0.17,P = 0.012),and the thymus indexes of the ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups were significantly higher than in the ADM group(0.20 ± 0.06 vs 0.13 ± 0.04,P = 0.029;0.47 ± 0.12 vs 0.13 ± 0.04,P = 0.000).APS was found to exert a synergistic antitumor effect with ADM and to alleviate the decrease in the sizes of the spleen and thymus induced by AMD.The expression of interleukin-1α(IL-1α),IL-2,IL-6,and tumor necrosis factor-α(TNF-α) was significantly higher in the ADM + APS(50 mg/kg),ADM + APS(100 mg/kg) and ADM + APS(200 mg/kg) groups than in the ADM group;and IL-10 was significantly lower in the above groups than in the ADM group.APS could increase IL-1α,IL-2,IL-6,and TNF-α expression and decrease IL-10 levels.Compared with the ADM group,APS treatment at a dose of 50-200 mg/kg could downregulate MDR1 mRNA expression in a dose-dependent manner(0.48 ± 0.13 vs 4.26 ± 1.51,P = 0.000;0.36 ± 0.03 vs 4.26 ± 1.51,P = 0.000;0.21 ± 0.04 vs 4.26 ± 1.51,P = 0.000).The expression level of P-GP was significantly lower in the ADM + APS(200 mg/kg) group than in the ADM group(137.35 ± 9.20 mg/kg vs 282.19 ± 20.54 mg/kg,P = 0.023).CONCLUSION:APS exerts a synergistic anti-tumor effect with ADM in H22 tumor-bearing mice.This may be related to its ability to enhance the expression of IL1α,IL-2,IL-6,and TNF-α,decrease IL-10,and downregulate MDR1 mRNA and P-GP expression levels. | Qing-E Tian Huan-De Li Miao Yan Hua-Lin Cai Qin-You Tan Wen-Yuan Zhang | 2012 | World Journal of Gastroenterology2012，18，47： | 30 |
| 2 | 黄芪提取物的免疫调节及治疗作用(英文)显示文摘黄芪是一味传统的中草药,具有多样的医学功效,被中国及其他亚洲国家广泛使用。其在疾病治疗及健康调理方面具有悠久的应用历史。近年来,大量研究对其单一成分(如多糖)或混合提取物的作用机制进行了探索。本文就其对机体免疫反应的影响作用此一方面进行了综述。综述讨论了其对固有性、获得性免疫反应以及细胞因子产生的影响,另一方面,就其在抗肿瘤、抗病毒以及作为佐剂的潜能方面进行了探讨。 | 赵冰 杜小刚 曾宪垠 | 2013 | 中国抗生素杂志2013，38，9： | 13 |
| 3 | 黄芪皂苷对人胃癌细胞系MKN-74增殖与侵袭的影响显示文摘目的探讨黄芪属植物黄芪的根部的一种提取物——黄芪皂苷(astragaloside,AST)在体外对胃癌MKN-74细胞增殖与侵袭力的影响。方法用五个不同剂量(0,2.5,5,10,20μmoL.L-1)的和胃癌MKN-74细胞共培养24、48、72小时,通过MTT法检测黄芪皂苷(AST)对细胞的抑制作用;Transwell侵袭实验检测黄芪皂苷对MKN-74细胞侵袭力的抑制作用。结果 (1)AST在体外能够显著抑制胃癌MKN-74细胞的增殖,2.5μmoL.L-1,5μmoL.L-1,10μmoL.L-1,20μmoL.L-1黄芪皂苷处理72小时生长抑制率分别为4.65%,7.58%,20.73%,30.38%,呈现明显的剂量和时间依赖性。(2)AST显著抑制MKN-74细胞的侵袭力,呈现剂量和时间依赖性。结论 AST在体外对人胃癌MKN-74细胞有显著的抑制细胞增殖作用。 | 王湘华 刘小军 蒋艳 | 2012 | 肿瘤药学2012，2，1： | 10 |
| 4 | 胃肠癌腹膜转移的研究进展显示文摘腹膜转移作为胃肠癌的一种最常见的转移形式,其发生发展过程有着复杂的机制。作者以经典的种子-土壤学说为切入点,对近些年国内外学者对腹膜转移中诸多问题的研究进展进行了系统的阐述。 | 徐惠绵 | 2015 | 中华结直肠疾病电子杂志2015，4，1： | 9 |
| 5 | A Systematic Review of Anticancer Effects of Radix Astragali显示文摘Objective:To review the anticancer effects of Radix astragali(RA),one of the most commonly used herbs to manage cancer in East Asia,and its constituents and to provide evidence of clinical usage through previously performed clinical studies.Methods:Preclinical and clinical studies related to the anticancer effects of RA were searched from inception to November 2013 in electronic databases.Two reviewers independently investigated 92 eligible studies,extracted all the data of studies and appraised methodological quality of clinical trials.The studies were categorized into in vitro and in vivo experimental studies and clinical studies,and analyzed by saponins,polysaccharides,and flavonoids of RA constituents,RA fraction,and whole extract.Results:In preclinical studies,RA was reported to have tumor growth inhibitory effects,immunomodulatory effects,and attenuating adverse effects by cytotoxic agents as well as chemopreventive effects.Saponins seemed to be the main constituents,which directly contributed to suppression of tumor growth through the activation of both intrinsic and extrinsic apoptotic pathway,modulation of intracellular signaling pathway,and inhibition of invasion and angiogenesis.Flavonoids suppressed tumor growth through the similar mechanisms with saponins.Polysaccharides showed immunomodulatory effects,contributing tumor shrinkages in animal models,despite the low cytotoxicity to cancer cells.Most of the clinical studies were performed with low evidence level of study designs because of various limitations.RA whole extracts and polysaccharides of RA were reported to improve the quality of life and ameliorate myelosuppression and other adverse events induced by cytotoxic therapies.Conclusion:The polysaccharides,saponins,and flavonoids of RA,and the whole extract of RA have been widely reported with their anticancer effects in preclinical studies and showed a potential application as a adjunctive cancer therapeutics with the activities of immunomodulation,anti-proliferation and attenuation of adverse effects induced by cytotoxic therapy. | Yeehong Jung Uimin Jerng Sookyung Lee | 2016 | Chinese Journal of Integrative Medicine2016，22，3： | 9 |
| 6 | 地锦草对Hela细胞增殖与凋亡的影响显示文摘目的:探讨中药地锦草对Hela细胞增殖和凋亡作用的影响。方法:以人宫颈癌Hela细胞株为研究对象,采用MTT法检测地锦草对Hela细胞增殖抑制作用的影响,AO/EB染色法荧光倒置显微镜观察药物作用后凋亡小体的形成,Annexin V-FITC流式细胞术(FCM)检测细胞凋亡变化情况。结果:地锦草能够抑制Hela细胞的体外增殖,抑制程度随药物浓度50μg·m L^(-1)升高到200μg·m L^(-1),抑制率也由30.4%增大到61.1%,显示了良好的浓度依存关系(P<0.05);AO/EB染色法显示药物作用后凋亡小体形成,FCM法结果显示随着药物作用质量浓度由0增高到200μg·m L^(-1),细胞凋亡率也由0.76%升高到57.17%(P<0.05)。结论:地锦草能够抑制Hela细胞增殖。其作用可能是通过诱导Hela细胞凋亡和分化实现的。 | 江涛 周兵 付丹 利园梦 | 2017 | 中国现代中药2017，19，12： | 8 |
| 7 | 黄芪多糖通过JAK/STAT3通路抑制口腔鳞癌SCC-25裸鼠移植瘤显示文摘目的阐明黄芪多糖(AP)对口腔鳞癌(oral squamous cell carcinoma)细胞系SCC-25移植瘤模型小鼠的影响和机制。方法皮下注射口腔鳞癌细胞系SCC-25建立异种移植瘤模型,将移植瘤BALB/c裸鼠模型随机分为4组:对照组(cTRL)、AP低剂量组(AP 10 mg)、AP中剂量组(AP 25 mg)和AP高剂量组(AP 50 mg)。AP治疗后,检测移植瘤体积和小鼠存活率;免疫组化检测Ki67和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达水平;TUNEL实验检测移植瘤细胞凋亡情况;Western blot检测JAK2/STAT3通路蛋白表达情况和磷酸化情况。结果与对照组相比较,AP低、中、高剂量组肿瘤体积明显减小(P<0.01),小鼠存活率明显增加(P<0.01),Ki67和VEGF表达水平明显下降(P<0.01),JAK2/STAT3/c-myc磷酸化水平明显被抑制(P<0.01)。结论 AP以剂量依赖抑制SCC-25移植瘤体积,提高移植瘤小鼠存活率,下调Ki67和VEGF表达水平和JAK2/STAT3磷酸化水平。AP可能通过抑制JAK2/STAT3/c-myc信号通路抑制口腔鳞癌细胞SCC-25移植瘤生长。 | 邓力 蔡婷 王静雷 夏雨 周智 | 2019 | 中国临床解剖学杂志2019，37，2： | 8 |
| 8 | 胃癌腹膜转移机理及其临床应用的研究进展显示文摘胃癌是发病率死产率均较高的恶性肿瘤,亚洲是高发区,每年有约30万人死于胃癌,严重危害着同人的健康。腹膜转移是胃癌最常见的转移形式之一,约有50%的术后复发病例为腹膜转移,是导致患者死广的主因。深入了解胃癌腹膜转移的发病机制、发展过程,对治疗和预后均至关重要。但是,胃癌腹膜转移的发病机制十分复杂,涉及及粘附、降解、移动、 | 徐惠绵 武建华 | 2012 | 中国肿瘤临床2012，29，22： | 7 |
| 9 | Efficacy and safety of the Qiguiyin formula in severe pneumonia: study protocol for a randomized, double-blind, placebo-controlled clinical trial显示文摘OBJECTIVE: To evaluate the clinical efficacy and safety of Qiguiyin(QGY) formula in patients with severe pneumonia in China compared with a placebo.METHODS: This is a multicenter double-blind, placebo-controlled, randomized clinical trial with two parallel arms. There will be 530 patients enrolled and randomized into either the experimental group(QGY formula) or the control group(placebo). Therapies for patients in the two groups above will be based on the conventional therapy. The primary outcome is 28-day mortality. Secondary outcomes include:(a) duration of hospital stay;(b) duration of time in the intensive care unit(ICU) stays;(c) duration of mechanical ventilation;(d) antibiotic DDD value(which means the doses of antibotics during the treatment period);(e) serum procalcitonin(PCT) level;(f) serum C-reactive protein(CRP)level;(g) Pneumonia severity index(PSI) score;(h)Sequential Organ Failure Assessment(SOFA) score;(i) sputum culture results;(j) blood routine examination results;(k) routine urine test results;(l) stool routine examination results;(m) electrocardiogram results;(n) alanine aminotransferase levels;(o) aspartate amino transferase levels;(p) total bilirubin;(q) creatinine levels;(r) urea nitrogen levels;and(s)adverse events.ETHICS AND DISSEMINATION: The protocol has been approved by the Research Ethics Committee of Beijing Hospital of Traditional Chinese Medicine,Affiliated with Capital Medical University(2018 BL-053-02). This trial aims to provide evidence for QGY formula combined with conventional therapy in treating patients with severe bacterial pneumonia,and to verify the clinical effectiveness and safety of QGY formula in China compared with placebo. Additionally, this trial will reveal the effect of QGY formula on delaying/reversing the characteristics of drug-resistant bacteria.Trial registration number: Chi CTR1800019785. | Huang Po Guo Yuhong Zhao Jingxia Li Bo Wu Yanqing Liu Qingquan | 2020 | Journal of Traditional Chinese Medicine2020，40，2： | 6 |
| 10 | 毛蕊异黄酮对缺氧缺糖/复氧复糖PC12细胞线粒体凋亡通路的影响显示文摘目的探讨毛蕊异黄酮对缺氧缺糖/复氧复糖PC12细胞线粒体凋亡通路的影响。方法将PC12细胞随机分为4组:正常组(control)、模型组(model)、毛蕊异黄酮组(calycosin)和尼莫地平组(nimodipine,阳性对照药组)。除control组外,其余各组进行缺氧缺糖2 h,复氧复糖24 h制作模型处理。Calycosin组和nimodipine组在复氧复糖的同时分别给予含有毛蕊异黄酮(0.07μmol/L)和尼莫地平(5.00μmol/L)的含药培养基处理。用CCK-8法检测各组细胞活性,流式细胞术检测各组细胞凋亡率,细胞免疫荧光法检测各组细胞Bax/Bcl-2比值,Western blotting法检测线粒体凋亡通路中关键蛋白细胞色素C(Cyt-C)、凋亡酶激活因子1(Apaf-1)和Caspase-3表达。结果与control组相比,model组细胞的活性明显降低(P<0.05),细胞凋亡率明显升高(P<0.05),Bax/Bcl-2比值明显升高(P<0.05),线粒体凋亡通路中关键蛋白Cyt-C、Apaf-1和Caspase-3的表达均明显升高(P<0.05);与model组相比,calycosin组和nimodipine组的细胞活性均明显提高(P<0.05),细胞凋亡率明显降低(P<0.05),Bax/Bcl-2比值明显降低(P<0.05),线粒体凋亡通路中关键蛋白Cyt-C、Apaf-1和Caspase-3的表达均明显降低(P<0.05),差异有统计学意义。结论毛蕊异黄酮可显著提高缺氧缺糖/复氧复糖PC12细胞活性,抑制细胞凋亡,其作用机制与毛蕊异黄酮抑制线粒体凋亡通路中关键蛋白Cyt-C、Apaf-1和Caspase-3表达密切相关。 | 张彐宁 靳晓飞 周晓红 高维娟 | 2020 | 解剖学报2020，51，3： | 4 |
| 11 | 胃癌腹膜亚临床转移的诊治研究显示文摘胃癌是我国发病率和死亡率最高的恶性肿瘤之一,每年新增病例约30余万,其中死亡人数达17万左右。在目前住院病例中,90%以上为进展期胃癌,即使施行根治手术,5年生存率仍徘徊在30%左右。根治术后腹膜种植性转移占40%~50%,是影响预后的首要因素。因此,掌握胃癌腹膜转移规律,确立亚临床转移的早期诊断指标或预测指标及防治靶点,建立有效的预防和综合治疗体系,是提高远期疗效的关键, | 徐惠绵 | 2011 | 外科理论与实践2011，16，3： | 4 |
| 12 | 细胞因子诱导的杀伤细胞在感染性疾病免疫治疗中的作用显示文摘细胞因子诱导的杀伤细胞(cytokine-induced killer cells,CIK细胞)是体外采用多种细胞因子扩增的异质性细胞群,具有增殖活性高、杀瘤活性高、非MHC限制性、安全性高等特点。此外,CIK细胞治疗可明显改善肿瘤患者免疫抑制状态,提高机体的免疫功能。CIK细胞在乙型肝炎、丙型肝炎、EB病毒相关的移植后淋巴组织增生性疾病等非肿瘤性疾病中也有应用研究的相关报道。CIK细胞过继免疫治疗有助于提高机体免疫功能,可有效抑制肝炎病毒复制、降低肝炎病毒载量、减轻肝脏炎症。本文就CIK细胞在感染相关性疾病治疗中的应用作一综述。 | 张克新 王智刚 蒋敬庭 | 2013 | 临床检验杂志2013，31，3： | 3 |
| 13 | DC-CIK疗法联合化疗治疗晚期非小细胞肺癌显示文摘目的研究DC-CIK疗法联合化疗治疗晚期非小细胞肺癌的疗效及安全性。方法 52例晚期非小细胞肺癌患者(DC-CIK组)应用DC-CIK疗法联合化疗治疗,同期52例(对照组)仅给予化疗,比较2组疗效和安全性。结果 DC-CIK组患者的有效率、疾病控制率分别为26.9%、82.7%,显著高于对照组的9.6%、61.5%(P<0.05);DC-CIK组和对照组中位生存期分别为12.5、11.3个月(P<0.05)。DC-CIK组患者的毒副反应明显轻于对照组(P<0.05)。结论在晚期非小细胞肺癌的治疗中,DC-CIK疗法联合相较于单纯化疗更安全、更有效。 | 秦亚光 程传耀 王亚秋 卢红 | 2015 | 肿瘤基础与临床2015，28，6： | 3 |
| 14 | 黄芪对嗜酸乳杆菌生长及其产酶活的影响显示文摘研究添加不同浓度的黄芪提取液对嗜酸乳杆菌生长及产酶能力的影响,对比在改良的MRS培养基中添加与未添加黄芪提取液对嗜酸乳杆菌的生物量和生长周期的影响,同时测定了嗜酸乳杆菌产β-半乳糖苷酶、葡萄糖氧化酶和淀粉酶的酶活,确定黄芪提取液的最佳添加浓度。结果表明:当添加黄芪提取液浓度为0.032mg/mL时对嗜酸乳杆菌生长有明显的促进作用,且在此浓度下pH达最低值3.88;当添加黄芪提取液浓度为0.016g/mL时,嗜酸乳杆菌产β-半乳糖苷酶、葡萄糖氧化酶和淀粉酶的酶活达到最高值,分别为387.56、14.42、4.4U。 | 张庆庆 李慧静 曹乾升 赵爱兵 陈剑翔 金鑫强 | 2013 | 食品工业科技2013，34，15： | 2 |
| 15 | 川芎嗪对小鼠Lewis细胞增殖与凋亡的影响显示文摘目的探讨中药川芎嗪对小鼠Lewis细胞的增殖和凋亡作用的影响。方法采用噻唑蓝法检测川芎嗪对Lewis细胞增殖的抑制作用,倒置显微镜观察药物作用前后细胞形态学的变化情况,流式细胞术(FCM)观察Lewis细胞凋亡指标的变化。结果川芎嗪能够抑制Lewis细胞的体外增殖,12.5、25.0、50.0 mg·L-1组的抑制率分别为29.8%、50.5%、61.3%,其48 h半数抑制浓度(IC50)为33.504 mg·L-1。实验组Lewis细胞经12.5、25.0和50.0 mg·L-1的川芎嗪处理48 h后,其凋亡率分别为(19.23±0.17)%、(30.81±0.46)%、(55.26±0.19)%;对照组为(0.64±0.37)%,实验组与对照组比较差异均有统计学意义(P<0.05)。结论川芎嗪能够抑制Lewis细胞增殖,其作用可能是通过诱导Lewis细胞凋亡和分化实现的。 | 郭久宏 彭胜国 康玉斌 | 2014 | 新乡医学院学报2014，31，1： | 2 |
| 16 | 乳斑肿瘤相关巨噬细胞诱导人腹膜间皮HMR-sv5细胞损伤的机制研究显示文摘目的:研究胃癌细胞对巨噬细胞的诱导作用,分析活化巨噬细胞对间皮细胞的损伤作用及机制。方法:人正常胃腺上皮细胞系GES-1及人低分化胃癌细胞系SGC-7901与人单核巨噬细胞THP-1共培养,诱导后者分化,研究后者对人腹膜间皮细胞HMR-sv5的损伤作用及分子机制。结果:胃癌细胞诱导THP-1形成肿瘤相关巨噬细胞(TAM),M1型巨噬细胞表面抗原的表达显著下调,而M2表型的表面抗原明显上调,细胞形态也发生明显改变。TAM显著抑制正常间皮细胞生长,促进间皮细胞凋亡和上皮间质转化。结论:胃癌细胞诱导巨噬细胞发生表型和功能转化,进而导致间皮细胞发生EMT和凋亡,促进形成腹膜转移癌。 | 刘省宇 苗智峰 武剑华 由一 徐昊 王振宁 徐惠绵 | 2012 | 中国肿瘤临床2012，29，22： | 1 |
| 17 | 腹腔中由游离癌细胞分泌的TGF-β1影响腹膜间皮形态与功能进而促进癌转移的研究显示文摘目的:研究胃癌分泌的转化生长因子-β1(TGF-β1)对腹膜的破坏机制及TGF-β1抑制剂对腹膜的保护。方法:ELISA法检测胃癌分泌上清中的相关细胞因子含量及在胃癌-腹膜相互作用中TGF-β-Smad信号通路蛋白的水平。观察TGF-β1抑制剂SB431542对腹膜癌转移的抑制作用。结果:5种胃癌细胞系均能分泌大量TGF-β1,TGF-β1的分泌量明显多于其他细胞因子。Western证实了TGF-β1及其信号通路TGF-β1/Smad 2、3参与胃癌对间皮的损伤。胃癌上清能破坏间皮层,致损伤脱落,与TGF-β1共培养能明显影响间皮细胞生长。TGF-β1抑制剂SB431542可阻止胃癌上清对间皮的损伤。结论:胃癌上清中TGF-β1高表达,影响腹膜间皮形态与功能进而促进了癌转移,机制可能与Smad信号通路有关。TGF-β1抑制剂能减轻胃癌上清对间皮的损伤。 | 那迪 刘福囝 姜成钢 徐昊 王振宁 徐惠绵 | 2013 | 现代肿瘤医学2013，21，3： | 0 |
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