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1Review on microbial degradation of zearalenone and aflatoxins显示文摘The widespread contamination by mycotoxins,especially zearalenone and aflatoxins,in crops and their by-products has caused severe undesirable effects on human health and commercial trade.Researchers had screened out microorganisms from various media for degrading zearalenone and aflatoxins,and the results of a lot of studies showed that enzymes derived from microbial strains play a key role in degrading mycotoxins.Genetic engineering technology had been applied to improve the heterologously expressed degrading enzymes in several mature microbial hosts such as Escherichia coli and Pichia pastoris.The separated and purified recombinant enzyme had high activity in degrading mycotoxins in vitro.This review summarized the types of mycotoxins-degrading microorganisms and enzymes,and the progress on synthesis of heterologously expressed degrading enzymes by genetic engineering technology as well as related researches on improving the effect of degrading enzymes.We also prospected the future development in the study of using recombinant enzymes formed by genetic engineering technology to realize the simultaneous degradation of multiple mycotoxins in crops.Jiaxing Wang Yanli Xie 2020Grain & Oil Science and Technology2020,3,3:3
2黄曲霉毒素的提取、检测及防治方法研究进展显示文摘黄曲霉毒素是一类主要由黄曲霉和寄生曲霉分泌的次级代谢产物,具有致癌、致畸和致突变的作用,主要损害机体的肝脏和肾脏等组织,对人类危害极大。该文介绍了黄曲霉毒素的危害、提取和检测方法,并综述了黄曲霉毒素的防治方法,为进一步加强黄曲霉毒素的控制提供参考。李从虎 刘毅然 李肖纯 邢朝凤 王志勇 黄晓平 2015中国酿造2015,34,10:3
3细菌M19培养条件对降解黄曲霉毒素B_1的影响显示文摘对经筛选的一株降解黄曲霉毒素B_1(AFB_1)的菌株M19进行16S rDNA基因序列分析及系统发育树分析,从而进行菌株鉴定;以AFB_1降解率为评价指标研究培养条件(培养温度、培养时间、接种量、培养基的初始pH、培养基的装液量)对AFB_1降解率的影响。结果表明,该菌株为铜绿假单胞菌(Pseudomonas aeruginosa);培养温度和培养基初始pH对AFB_1降解率的影响较为显著,不同温度及不同初始pH条件下AFB_1降解率存在明显差别(P<0.05),而培养时间、装液量、接种量对AFB_1降解率的影响不显著。培养温度37℃时,AFB_1降解率为90%;初始pH为5时,AFB_1降解率为95.2%。宋娟娟 谢岩黎 王威 2018食品科技2018,43,7:2
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