共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Synergetic anticancer effect of combined gemcitabine and photodynamic therapy on pancreatic cancer in vivo显示文摘AIM:To investigate the anti-tumor effects of combined cytotoxic drug(gemcitabine)and photodynamic therapy(PDT)on human pancreatic cancer xenograft in nude mice. METHODS:Human pancreatic cancer cell line SW1990 was used in the investigation of the in vivo effect of combined gemcitabine and PDT on human pancreatic cancer xenograft in mice.Sixty mice were randomly allocated into a control group(without treatment),photosensitizer treatment group(2 mg/kg photosan,without illumination),chemotherapy group (50 mg/kg gemcitabine i.p.),PDT group(2 mg/kg photosan+laser irradiation)and combined treatment group(photosan+chemotherapy),with 12 mice in each group.Tumor size was measured twice every week.Anti-tumor activity in different groups was evaluated by tumor growth inhibition(TGI). RESULTS:No significant anti-tumor effect was observed either in photosensitizer treatment group or in chemotherapy group.PDT led to necrosis in cancer lesions and significantly reduced tumor volume compared with photosensitizer on day 6 and at the following time points after initialization of therapy (0.24±0.15-0.49±0.08 vs 0.43±0.18-1.25± 0.09,P<0.05).PDT significantly reduced tumor volume in combined treatment group compared with photosensitizer treatment group(0.12±0.07-0.28 ±0.12 vs 0.39±0.15-1.20±0.11,P<0.05),small dose chemotherapy group(0.12±0.07-0.28±0.12 vs 0.32±0.14-1.16±0.08,P<0.05)and control group (0.12±0.07-0.28±0.12 vs 0.43±0.18-1.25±0.09, P<0.05).TGI was higher in the combined treatment group(82.42%)than in the PDT group(58.18%). CONCLUSION:PDT has a significant anti-tumor effect,which is maintained for a short time and can be significantly enhanced by small doses of gemcitabine. | Qi xie Lin Jia Yan-Hong Liu Cheng-Gang Wei | 2009 | World Journal of Gastroenterology2009，15，6： | 11 |
| 2 | p21^(WAF1)基因对人胰腺癌细胞系BxPC-3增殖的抑制作用显示文摘目的:探讨p21WAF1(p21)基因转染对人胰腺癌细胞系BxPC-3细胞增殖的影响.方法:根据转染质粒的不同和是否进行质粒转染分为3组,p21转染组、空载体转染组和未转染组.应用RT-PCR和Western blot方法检测转染细胞的p21基因表达变化;流式细胞仪分析细胞周期变化,用MTT、流式细胞仪和透射电镜检测转染外源p21基因对BxPC-3细胞增殖和凋亡的影响.结果:p21转染组细胞存在p21 mRNA高表达和P21蛋白高表达;p21转染组细胞生长速度低于对照空载体转染组和未转染组;流式细胞仪观察到P21蛋白高表达使BxPC-3细胞发生G1/S阻滞,G1期细胞比例显著高于空载体组和未转染组(59.887%±3.700% vs 47.443%±6.354%,49.223%±2.226%,P<0.05),S期显著低于空载体组和未转染组(21.277%±2.080% vs 35.247%±3.966%,36.013%±1.540%,P<0.01),并出现亚G1峰(凋亡峰).透射电镜亦发现p21转染组发生细胞凋亡.结论:p21基因转染可以抑制人胰腺癌细胞系BxPC-3细胞增殖并能诱导其发生细胞凋亡. | 张学彦 姜仪增 刘志强 景德怀 关景明 刘伟 | 2009 | 世界华人消化杂志2009，17，16： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员