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1应用甲状腺素片及饥饱失常法建立肝郁证大鼠模型的研究显示文摘目的探讨建立中医肝郁证大鼠模型的一种新的造模方法。方法依次采用甲状腺素片灌胃、饥饱失常法喂养,观察大鼠的行为学变化,测量大鼠肛温、并用夹心ELISA法检测其血浆五羟色胺(5-hydroxytrypta mine,5-HT)、去甲肾上腺素(norepinephrine,NE)、肾上腺素(epinephrine,E)水平的变化及逍遥散对其含量的影响。结果肝郁模型组及逍遥散组大鼠于造模第28天均出现了典型的心肝火旺之症,体温均升高,与空白对照组相比有显著性差异(P<0.05);于造模第44天,肝郁模型组大鼠血浆5-HT、NE、E水平均较前明显升高(P<0.05或P<0.01);且NE、E水平高于逍遥散组(P<0.01和P<0.05);于造模第56天肝郁模型组出现典型的肝郁证的行为学特点,逍遥散组大鼠血浆中5-HT水平低于肝郁模型组(P<0.01)。结论应用甲状腺素片及饥饱失常法建立的肝郁证大鼠模型基本符合中医肝郁证的特征。刘素蓉 曹瑾 吴喜利 王志勇 王敏 高艳琼 2009西安交通大学学报(医学版)2009,30,2:2
2Specific responses of monoamine neurotransmitters to various acute stressors显示文摘This study determined the composition of histamine,serotonin and dopamine using high performance liquid chromatography and electrochemical detection,and compared the changes in monoamine levels in plasma,the cortex and midbrain of mice exposed to acute stressors,such as blood-drawing stimulation or restraint.Results demonstrated that plasma histamine levels were markedly increased when mice were exposed to blood-drawing stimulation and restraint stress.However,serotonin levels decreased in plasma of mice treated with restraint stress,and dopamine levels in plasma had no significant response to the two acute stressors.The three monoamines (histamine,serotonin and dopamine) increased at different degrees in restraint mice,but not in brain regions of blood-drawing stressed mice.Results indicated that histaminergic,serotonergic or dopaminergic systems have their own specific response to different acute stressors.Rongrong He Guanyu Lin Yifang Li Keiich Abe Xinsheng Yao Hiroshi Kurihara 2011Neural Regeneration Research2011,6,14:1
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