| 1 | Intravoxel incoherent motion diffusion-weighted imaging for monitoring chemotherapeutic efficacy in gastric cancer显示文摘AIM: To assess intravoxel incoherent motion diffusionweighted imaging(IVIM-DWI) for monitoring early efficacy of chemotherapy in a human gastric cancer mouse model.METHODS: IVIM-DWI was performed with 12 b-values(0-800 s/mm2) in 25 human gastric cancer-bearing nude mice at baseline(day 0), and then they were randomly divided into control and 1-, 3-, 5- and 7-d treatment groups(n = 5 per group). The control group underwent longitudinal MRI scans at days 1, 3, 5 and 7, and the treatment groups underwent subsequent MRI scans after a specified 5-fluorouracil/calciumfolinate treatment. Together with tumor volumes(TV), the apparent diffusion coefficient(ADC) and IVIM parameters [true water molecular diffusion coefficient(D), perfusion fraction(f) and pseudo-related diffusion coefficient(D*)] were measured. The differences in those parameters from baseline to each measurement(ΔTV%, ΔADC%, ΔD%, Δf% and ΔD*%) were calculated. After image acquisition, tumor necrosis, microvessel density(MVD) and cellular apoptosis were evaluated by hematoxylin-eosin(HE), CD31 and terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL) staining respectively, to confirm the imaging findings. Mann-Whitney test and Spearman's correlation coefficient analysis were performed.RESULTS: The observed relative volume increase(ΔTV%) in the treatment group were significantly smaller than those in the control group at day 5(ΔTV_(treatment)% = 19.63% ± 3.01% and ΔTVcontrol% = 83.60% ± 14.87%, P = 0.008) and day 7(ΔTV_(treatment)% = 29.07% ± 10.01% and ΔTV_(control)% = 177.06% ± 63.00%, P = 0.008). The difference in ΔTV% between the treatment and the control groups was not significant at days 1 and 3 after a short duration of treatment. Increases in ADC in the treatment group(ΔADC%_(treatment), median, 30.10% ± 18.32%, 36.11% ± 21.82%, 45.22% ± 24.36%) were significantly higher compared with the control group(ΔADC%_(control), median, 4.98% ± 3.39%, 6.26% ± 3.08%, 9.24% ± 6.33%) at days 3, 5 and 7(P = 0.008, P = 0.016, P = 0.008, respectively). Increases in D in the treatment group(ΔD%_(treatment), median 17.12% ± 8.20%, 24.16% ± 16.87%, 38.54% ± 19.36%) were higher than those in the control group(ΔD%_(control), median-0.13% ± 4.23%, 5.89% ± 4.56%, 5.54% ± 4.44%) at days 1, 3, and 5(P = 0.032, P = 0.008, P = 0.016, respectively). Relative changes in f were significantly lower in the treatment group compared with the control group at days 1, 3, 5 and 7 follow-up(median,-34.13% ± 16.61% vs 1.68% ± 3.40%, P = 0.016;-50.64% ± 6.82% vs 3.01% ± 6.50%, P = 0.008;-49.93% ± 6.05% vs 0.97% ± 4.38%, P = 0.008, and-46.22% ± 7.75% vs 8.14% ± 6.75%, P = 0.008, respectively). D* in the treatment group decreased significantly compared to those in the control group at all time points(median,-32.10% ± 12.22% vs 1.85% ± 5.54%, P = 0.008;-44.14% ± 14.83% vs 2.29% ± 10.38%, P = 0.008;-59.06% ± 19.10% vs 3.86% ± 5.10%, P = 0.008 and-47.20% ± 20.48% vs 7.13% ± 9.88%, P = 0.016, respectively). Furthermore, histopathologic findings showed positive correlations with ADC and D and tumor necrosis(r_s = 0.720, P < 0.001; r_s = 0.522, P = 0.007, respectively). The cellular apoptosis of the tumor also showed positive correlations with ADC and D(r_s = 0.626, P = 0.001; r_s = 0.542, P = 0.005, respectively). Perfusionrelated parameters(f and D*) were positively correlated to MVD(r_s = 0.618, P = 0.001; r_s = 0.538, P = 0.006, respectively), and negatively correlated to cellular apoptosis of the tumor(r_s =-0.550, P = 0.004; r_s =-0.692, P < 0.001, respectively).CONCLUSION: IVIM-DWI is potentially useful for predicting the early efficacy of chemotherapy in a human gastric cancer mouse model. | Xiao-Li Song Heoung Keun Kang Gwang Woo Jeong Kyu Youn Ahn Yong Yeon Jeong Yang Joon Kang Hye Jung Cho Chung Man Moon | 2016 | World Journal of Gastroenterology2016，22，24： | 12 |
| 2 | 体素内不相干运动弥散加权成像联合波谱分析对前列腺癌的诊断价值显示文摘目的探讨体素内不相干运动(IVIM)弥散加权成像联合磁共振波谱分析(MRS)对前列腺癌(PCa)的诊断价值。方法经病理证实的37例PCa,49例良性前列腺增生(BPH)及21例正常前列腺组织患者纳入研究中,所有患者均行IVIM及MRS检查。计算并比较分析三组病例的纯弥散系数(D)、伪弥散系数(D*)、灌注分数(f)及(胆碱+肌酸)/枸橼酸盐(CC/C)值的差异。通过Logistic回归分析建立受试者工作特征(ROC)曲线,对比分析D、f、CC/C、IVIM、MRS、IVIM+MRS诊断PCa的ROC曲线下面积(AUC),计算IVIM、MRS、IVIM+MRS诊断PCa的敏感度、特异度。结果所有患者获得可分析的ROI共154个(包括PCa 46个,BPH 80个,正常前列腺组织28个)。PCa D值的中位数(四分位间距)[M(P25,P75)]值[0.86×10-3 mm2/s(0.69×10-3 mm2/s,1.01×10-3 mm2/s)]显著低于BPH和正常前列腺组织[1.24×10-3 mm2/s(1.13×10-3 mm2/s,1.52×10-3 mm2/s),1.47×10-3 mm2/s(1.37×10-3 mm2/s,1.72×10-3 mm2/s)](U=-11.340,P=0.000;U=-9.364,P=0.000),PCa f值的M(P25,P75)值[3.85%(2.91%,4.97%)]显著高于BPH和正常前列腺组织[2.15%(1.58%,2.70%)、1.89%(1.39%,2.48%)](U=-8.948,P=0.011;U=-6.807,P=0.000),PCa CC/C值的M(P25,P75)值[2.93(2.06,3.93)]显著高于BPH和正常前列腺组织[0.53(0.42,0.65)、0.49(0.40,0.55)](U=-8.232,P=0.000;U=-6.262,P=0.000),D*值无统计学差异(P>0.05)。各组诊断方法的AUC、敏感度和特异度分别为:IVIM:0.968、93.4%和91.7%;MRS:0.928、89.1%和93.5%,CC/C的最佳阈值为0.98;IVIM+MRS:0.991、95.8%和96.3%。结论 IVIM及MRS两种方法均可独立有效诊断PCa,两种技术结合较单独的任何一种更有助于PCa的诊断。 | 孔晓勤 邢可舟 赵新尧 葛亚平 郭文彬 郑应心 章涛 史浩 | 2016 | 中华临床医师杂志(电子版)2016，10，12： | 7 |
您所在的机构暂未开通此部分的功能，请联系管理员