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1盾叶薯蓣内生菌研究进展显示文摘植物内生菌是与植物协同进化的一大类微生物种群,具有抵抗病原微生物、促进宿主生长、产与宿主相似的次生代谢物等多种有益特性,是宝贵的生物资源库。盾叶薯蓣具有很高的药用价值,也是多种激素类药物合成前体的主要植物来源。从盾叶薯蓣植物分离具有有益特性的内生菌成为近年内生菌研究的热点。本文从盾叶薯蓣内生菌的分离鉴定方法和盾叶薯蓣内生菌的特性两个方面概述了近年来盾叶薯蓣内生菌的研究现状,以期为盾叶薯蓣内生菌的进一步研究开发提供参考。王越 王春慧 张琰 李冠楠 乔亚茹 王艳 何苗 化文平 2015基因组学与应用生物学2015,34,8:3
2Production of secondary metabolite E2.2 from Phaleria macrocarpa endophytic fungus显示文摘Objective: To isolate new endophytic fungus from Phaleria macrocarpa(P. macrocarpa)that is able to produce E2.2 compound.Methods: Endophytic fungi were isolated from P. macrocarpa. Morphological and molecular identification was done to determine the species of the endophytic fungus.High performance liquid chromatography was used to determine the ability of this fungus to produce E2.2 compound and to quantify the total yield of E2.2 from fungal fermentation. Fermentation process was optimized by observing suitable medium, p H and length of fermentation process. Phloroglucinol and gallic acid addition were examined to determine the effect of each compound on E2.2 production.Results: One endophytic fungus was successfully isolated from P. macrocarpa plant.Morphological and molecular identification showed that it was a Colletotrichum gloeosporioides which belonged to Glomerellaceae family. This fungus showed highest production of E2.2 when incubated in potato dextrose broth with initial p H value of the medium at 5, and was incubated for 15 days. Phloroglucinol was found to better enhance E2.2 production.Conclusions: Colletotrichum gloeosporioides found in P. macrocarpa plant is promising as a potential alternative source of E2.2.Beatrix Trikurnia Gasong Raymond Rubianto Tjandrawinata 2016Asian Pacific Journal of Tropical Biomedicine2016,6,10:0
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