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| 1 | 小麦TaCIPK2基因克隆及与TaCBLs蛋白互作分析显示文摘以普通六倍体小麦品种德抗961为材料,根据野大麦HbCIPK2的mRNA序列(GenBank序列号JN831652)设计引物,采用同源克隆方法从小麦中克隆TaCIPK2基因(GenBank序列号KU640381),测序结果显示该基因序列全长1421bp,开放阅读框(ORF)1359bp,编码452个氨基酸残基,预测分子量为50.91kD,pI为9.07。氨基酸序列比对结果表明:该基因与HvCIPK2(KP638475.1)、TaCIPK2(KJ561791.1)、HbCIPK2(JN831652.1)的相似度分别为94.69%、96.93%、95.80%,具有高度同源性;系统进化分析表明它们位于相同进化支上,亲缘关系最近。采用Real-timePCR方法分析不同逆境胁迫处理下TaCIPK2基因表达的特异性,200mmol/LNaCl高盐胁迫处理小麦幼苗后根和叶部TaCIPK2基因均上调表达;4℃低温胁迫处理后根部TaCIPK2基因上调表达,而叶部下调表达;100μmol/LABA胁迫处理后根和叶中TaCIPK2均上调表达。为检测TaCIPK2与TaCBL1、TaCBL2、TaCBL6、TaCBL7的相互作用,构建酵母表达载体pGADT7-TaCIPK2,转化酵母Y187感受态细胞;同理pGBKT7-TaCBL1、pGBKT7-TaCBL2、pGBKT7-TaCBL6、pGBKT7-TaCBL7转化到酵母细胞Y2Hgold中,都没有出现自激活活性和毒性现象。共转化的二倍体酵母,只有pGADT7-TaCIPK2×pGBKT7-TaCBL2和pGBKT7-53×pGADT7-T在SD/-Ade/-His/-Leu/-Trp/X-α-Gal/AbA培养基板上长出的菌落呈现蓝色,说明TaCIPK2能够与TaCBL2相互作用,激活下游报告基因HIS3、AUR1-C、MEL1和ADE2的表达,该研究结果对进一步研究TaCIPK2的功能有一定的指导意义。 | 程西永 王晓晓 苏鹏 董中东 任妍 詹克慧 许海霞 | 2016 | 分子植物育种2016，14，7： | 4 |
| 2 | Interplay between nitric oxide and sulfur assimilation in salt tolerance in plants显示文摘Nitric oxide(NO),a versatile molecule,plays multiple roles in plant growth and development and is a key signaling molecule in plant response to abiotic stress.Nutrient management strategy is critical for abiotic stress alleviation in plants.Sulfur(S) is important under stress conditions,as its assimilatory products neutralize the imbalances in cells created by excessive generation of reactive oxygen species(ROS).NO abates the harmful effects of ROS by enhancing antioxidant enzymes,stimulating S assimilation,and reacting with other target molecules,and regulates the expression of various stress-responsive genes under salt stress.This review focuses on the role of NO and S in responses of plants to salt stress,and describes the crosstalk between NO and S assimilation in salt tolerance.The regulation of NO and/or S assimilation using molecular biology tools may help crops to withstand salinity stress. | Mehar Fatma Asim Masood Tasir S.Per Faisal Rasheed Nafees A.Khan | 2016 | The Crop Journal2016，4，3： | 0 |
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