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| 1 | 大黄酸对高糖诱导人肾小球系膜细胞Wnt、β-catenin蛋白及TGF-β1表达的影响显示文摘目的观察高糖对人肾小球系膜细胞Wnt、β-catenin蛋白表达、TGF-β1分泌的影响及大黄酸的干预作用。方法体外培养人肾小球系膜细胞.同步化后采用加有高浓度葡萄糖(30 mmol/L)联合不同浓度大黄酸的无血清培养基培养。应用MTT法测量高糖(HG组)及大黄酸高浓度组(HG+R1组)、大黄酸中浓度组(HG+R2组)、大黄酸低浓度组(HG+R3组)干预后肾小球系膜细胞增殖活力。各组细胞中Wnt、β-catenin蛋白采用放射免疫法检测。各组细胞上清液中转化生长因子β1(TGF-β1)采用ELISA法检测。结果①对系膜细胞增殖的影响:与正常对照组(NG)组24、48、72 h比较.HG组、HG+R1组、HG+R2组、HG+R3组细胞增殖均明显上升,差异均有统计学意义(P<0.05或P<0.01);与HG组各时点相比.HG+R1组、HG+R2组、HG+R3组在24 h呈下降趋势.48、72 h下降趋势显著(P<0.05或P<0.01),表现为时间、浓度依赖性;HG+R2组在24、48 h细胞增殖较HG+R3组各时点有下降趋势.72h时细胞增殖下降趋势显著(P<0.05).而HG+R1组细胞在48h、72h时细胞增殖均显著下降(P<0.05);与相比,HG+R1组细胞增殖抑制作用在72h时较HG+R2组显著下降(P<0.05)。②NG组细胞胞浆中有少量Wnt、β-catenin表达,HG组胞核表达Wnt胞浆及β-catenin量明显增高(P<0.05).大黄酸干预组Wnt、β-catenin蛋白表达受到抑制(P<0.05)。③细胞分泌TGF-β1受高糖刺激增加,TGF-β1的分泌受大黄酸抑制,均呈现出浓度依赖性(.P<0.05或P<0.01)。结论大黄酸在体外可能抑制Wnt、β-catenin蛋白表达.从而抑制分泌TGF-β1. | 张涛 朱小军 陈慧 张明 吕韶燕 | 2015 | 环球中医药2015，8，S2： | 8 |
| 2 | Expression of serum Dickkopf-1 in gastric cancer patients显示文摘Objective:To explore the expression and clinical significance of DKK-1 protein in patients with gastric cancers.Methods:Enzyme linked imtnuno sorbent assay was used to detect expressions of serum DKK-1 protein in 90 cases of gastric cancers,50 cases of gastric benign disease and 40 healthy cases.The dynamic change in serum DKK-1 protein of gastric cancer patients who accepted radical operation for a month was also observed.Results:The expression of serum DKK-1 protein in gastric cancer groups was significantly higher than that in gastric benign group's(P<0.01) and in health control(P<0.01).Serum DKK 1 level was increased gradually along with the progress of the disease.Serum DKK 1 levels were significantly higher in patients at TNM staging Ⅲ and Ⅳ than patients at TNM staging Ⅰ andⅡ.Level of serum DKK-1 was related to microvascular invasion,differentiation degree and infiltration depth.Level of serum DKK-1 was significantly reduced in patients after radical surgery(P<0.01).Conclusions:The expression of serum DKK-1 protein in gastric cancer patients is increased.Level of serum DKK-1 is related to TNM staging,microvascular invasion,differentiation degree and infiltration depth.DKK-1 detection can be used as a reference index in monitoring gastric cancer progress and biological behavior. | Gui-Feng Zhuang Yan Tan Jun-Tao Zeng Jie-Wei Zhang Jing Tang Shi-Ping Zeng Xi Qin | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，10： | 4 |
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