共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effect of microRNA-155 on angiogenesis after cerebral infarction of rats through AT1R/VEGFR2 pathway显示文摘Objective:To explore the function and mechanism of microRNA-155 to regulate the angiogenesis after the cerebral infarction of rats through the angiotensin Ⅱ receptor 1(AT1R)/vascular endothelial growth factor(VEGF) signaling pathway.Methods:Female SD rats were chosen for the construction of cerebral infarction model of rats using the modified right middle cerebral artery occlusion.The real-time PCR(RT-PCR) method was employed to detect the expression of microRNA-155 in each group at different time points after the cerebral infarction(1 h,I d,3 d and 7 d).SD rats were randomly divided into four groups(n=20 rats):sham operation group(Sham group),MACO group,MACO+microRNA-155 mimic group,and MACO+microRNA-155 inhibitor group.Sham group was given the free graft,while MACO+microRNA-155 mimic group and MACO+microRNA-155 inhibitor group were treated with microRNA-155 mimic and microRNA-155 inhibitor respectively.The Zea Longa5-point scale was used to score the neurologic impairment of rats in each group;2,3,5-triphenyl tetrazolium chloride staining to evaluate the volume of cerebral infarction of rats in each group;the immunohistochemistry to detect the expression of CD31;Western blot and RT-PCR to detect the expression of AT1 R and VEGF receptor 2(VEGFR2).Results:The expression of microRNA-155 was increased in the cerebral ischemia tissue after the cerebral infarction.It was significantly increased at 1 d of ischemia and maintained at the high level for a long time.Rats in the Sham group had no symptom of neurologic impairment while rats in the MACO group had the obvious neurologic impairment.After being treated with microRNA-155 inhibitor,the neural function of MACO rats had been improved,with the decreased area of cerebral infarction.But after being treated with microRNA-155 mimic,the neural function was further worsened,with the increased area of cerebral infarction.Results of immunohistoehemical assay indicated that microRNA-155 inhibitor could up-regulate the expression of CD31,while microRNA-155 mimic could down-regulate the expression of CD31.The RT-PCR found that,after being treated with microRNA-155 inhibitor,MACO rats had the increased expression of ATIR and VEGFR2 messenger RNA(mRNA);but after being treated with microRNA-155 mimic,the expression of ATIR and VEGFR2 mRNA was decreased.Results of Western blot showed that,after being treated with microRNA-155 inhibitor,MACO rats had the increased expression of ATIR and VEGFR2 mRNA;but after being treated with microRNA-155 mimic,the expression of ATIR and VEGFR2 mRNA was decreased.Conclusions:The inhibition of microRNA-155 can improve the neurologic impairment of rats with the cerebral infarction,reduce the volume of cerebral infarction and effectively promote the angiogenesis in the region of ischemia,which may be mediated through AT1R/VEGFR2 pathway. | Ying-Chun Meng Zhen-Ying Ding Hai-Quan Wang Li-Ping Ning Chao Wang | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，10： | 10 |
| 2 | miRNA与颈动脉狭窄的研究进展显示文摘颈动脉是将血液从心脏输送到头颈及面部的大血管,是颅脑的主要供血血管之一。动脉粥样硬化、颈动脉夹层及与自身免疫、发育、炎症有关的血管等多种原因可以导致颈动脉狭窄。不同病因导致的颈动脉狭窄及临床表现亦不同。本文探讨miRNA在颈动脉狭窄发生、发展过程中的作用过程及机理,对miRNA与颈动脉狭窄的有关研究进行综述。 | 范星 周翔宇 | 2016 | 现代临床医学2016，42，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员