共被期刊论文引用了2次
您的检索式：您选中1篇文献正在查看引证文献汇总
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Annexin A2 silencing enhances apoptosis of human umbilical vein endothelial cells in vitro显示文摘Objective:To study the effects of inhibited Annexin A2(ANXA2) on human umbilical vein endothelial cells(HUVECs) in vitro.Methods:Short hairpin RNA(shRNA) targeting ANXA2 was designed and cloned into double marked lentvirial vector GV248 for RNAi to generate the recombinant expression plasmids,which were stably transfected into HUVECs.The protein and mRNA expression levels of ANXA2 were analyzed by western blotting and realtime polymerase chain reaction,respectively.Cell proliferation(cell counting kit-8 assay),apoptosis(flow cytometry analysis),the expression(western blotting) and the activity of easpases(enzyme-linked immunosorbent assay) were used to assess the effects of silencing ANXA2 on HUVECs in vitro.Results:The plasmids to express ANXA2-specific shRNA were constructed and were infected into HUVEC resulting in the stably transfected experimental(ANXA2-shRNA),control(eontrol-shRNA) and mock(no plasmid) cell lines,which were verified with western blot and real-time PCR.HUVEC/ANXA2-shRNA showed an inhibition rate 91.89%of ANXA2 expression compared to the mock HUVEC.ANXA2 silencing cell strain obviously presented a lower cell proliferation activity compared to the control and mock HUVECs,with an inhibition rate 82.35%on day 7 in vitro.FACS analysis indicated that the HUVEC/ANXA2-shRNA cells undergoing apoptosis increased by 102.61%compared to the mock HUVECs(P<0.01).Moreover,the activity levels of caspase-3,caspase-8 and caspase-9in HUVEC/ANXA2-shRNA cells were increased and the activated cleaved caspase-3,cleaved Caspase-8 and cleaved caspase-9 were upregulated evidently compared with that of the control and mock HUVECs by 56.29%,89.59%and 144.58%(P<0.01).Conclusions:shRNAmediated silencing of ANX A2 could not only be able to suppress HUVECs:proliferation but to upregulate the enzyme activity of easpases,which bring to an increase of cell apoptosis This work suggested that ANX A2 may represent a useful target of future molecular therapies. | Shu-Le Jiang Dong-Yan Pan Chao Gu Hai-Feng Qin Shi-Hong Zhao | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，11： | 4 |
| 2 | ANXA2对Caco2细胞凋亡的影响显示文摘为研究膜联蛋白A2(ANXA2)基因与人结直肠癌Caco2细胞凋亡之间的关系,采用RNAi技术沉默Caco2细胞的ANXA2表达后,利用流式细胞仪分析细胞凋亡水平,并利用激光共聚焦显微镜和透射电子显微镜对凋亡细胞进行形态学检测。流式细胞仪检测结果显示,ANXA2表达被抑制后细胞线粒体正常膜电位发生改变(P<0.05),线粒体结构受损;同时,形态学检测结果表明,ANXA2表达被抑制后细胞出现了较为明显的凋亡样特征。结果提示:ANXA2在人结直肠癌Caco2细胞的高表达能一定程度地促进细胞生长,抑制ANXA2表达可以诱导细胞出现一定程度的凋亡样形态特征,但不足以引起细胞广泛而明显的凋亡;推测ANXA2表达水平并不是唯一影响瘤细胞凋亡的因素。该结果支持关于ANXA2具有作为肿瘤靶向诊断、治疗靶分子潜能的推论。 | 奉艳 肖丽 何慧敏 高宁 石红燕 马乐乐 达苗苗 胥谨慧 许楠 刘雨恩 宋喜贵 侯颖春 | 2015 | 陕西师范大学学报(自然科学版)2015，43，1： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员