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| 1 | Broccoli sprout extract induces detoxification-related gene expression and attenuates acute liver injury显示文摘AIM: To investigate the effects of broccoli sprout extract(BSEx) on liver gene expression and acute liver injury in the rat.METHODS: First, the effects of BSEx on liver gene expression were examined. Male rats were divided into two groups. The Control group was fed the AIN-76 diet, and the BSEx group was fed the AIN-76 diet containing BSEx. After a 10-d feeding period, rats were sacrificed and their livers were used for DNA microarray and realtime reverse transcription-polymerase chain reaction(RT-PCR) analyses. Next, the effects of BSEx on acute liver injury were examined. In experiments using acute liver injury models, 1000 mg/kg acetaminophen(APAP) or 350 mg/kg D-galactosamine(D-Gal N) was used to induce injury. These male rats were divided into four groups: Control, BSEx, Inducer(APAP or D-Gal N), and Inducer+BSEx. The feeding regimens were identical for the two analyses. Twenty-four hours following APAP administration via p.o. or D-Gal N administration via i.p., rats were sacrificed to determine serum aspartate transaminase(AST) and alanine transaminase(ALT) levels, hepatic glutathione(GSH) and thiobarbituric acid-reactive substances accumulation and glutathioneS-transferase(GST) activity. RESULTS: Microarray and real-time RT-PCR analyses revealed that BSEx upregulated the expression of genes related to detoxification and glutathione synthesis in normal rat liver. The levels of AST(70.91 ± 15.74 IU/m L vs 5614.41 ± 1997.83 IU/m L, P < 0.05) and ALT(11.78 ± 2.08 IU/m L vs 1297.71 ± 447.33 IU/m L, P < 0.05) were significantly suppressed in the APAP + BSEx group compared with the APAP group. The level of GSH(2.61 ± 0.75 nmol/g tissue vs 1.66 ± 0.59 nmol/g tissue, P < 0.05) and liver GST activity(93.19 ± 16.55 U/g tissue vs 51.90 ± 16.85 U/g tissue, P < 0.05) were significantly increased in the APAP + BSEx group compared with the APAP group. AST(4820.05 ± 3094.93 IU/m L vs 12465.63 ± 3223.97 IU/m L, P < 0.05) and ALT(1808.95 ± 1014.04 IU/m L vs 3936.46 ± 777.52 IU/m L, P < 0.05) levels were significantly suppressed in the D-Gal N + BSEx group compared with the D-Gal N group, but the levels of AST and ALT in the D-Gal N + BSEx group were higher than those in the APAP + BSEx group. The level of GST activity was significantly increased in the D-Gal N + BSEx group compared with the D-Gal N group(98.04 ± 15.75 U/g tissue vs 53.15 ± 8.14 U/g tissue, P < 0.05).CONCLUSION: We demonstrated that BSEx protected the liver from various types of xenobiotic substances through induction of detoxification enzymes and glutathione synthesis. | Kazutaka Yoshida Yusuke Ushida Tomoko Ishijima Hiroyuki Suganuma Takahiro Inakuma Nobuhiro Yajima Keiko Abe Yuji Nakai | 2015 | World Journal of Gastroenterology2015，21，35： | 3 |
| 2 | 不同胁迫处理方法对西兰花茎中硫苷和异硫氰酸盐含量的影响显示文摘以西兰花(Brasica oleracea L.var.italica Plench)茎试材,研究不同的机械切分、4℃低温贮藏、热激和浸泡胁迫处理对其中的硫苷和异硫氰酸盐(ITCS)含量的影响,结果表明:机械切分、低温贮藏、热激和浸泡胁迫处理对其中的硫苷和异硫氰酸盐的含量均有显著的影响(P<0.05)。切分尺寸为(1.0×0.2×0.5)cm时,茎中硫苷的含量最低为22.85μmol/100 g DW,异硫氰酸盐的含量最高为653.74 mg/g DW。4℃下贮藏时,随着贮藏时间的延长与对照组相比硫苷降解和异硫氰酸盐生成的速度越来越慢,贮藏时间为8h时,硫苷降解和异硫氰酸盐生成的速率达到最低。在25℃下,将西兰花茎浸泡在不同p H的磷酸-柠檬酸缓冲液、Zn2+浓度和抗坏血酸溶液中2 h,发现p H 7.5缓冲液浸泡西兰花茎中硫苷的含量最低,为19.65μmol/100g DW,异硫氰酸盐的含量最高,为654.36mg/g DW。Zn2+浓度为100μmol/L时,硫苷的含量达到最低,为19.09μmol/100g DW达到最低,异硫氰酸盐的含量最高为为608.32 mg/g DW。抗坏血酸浓度为50μmol/L时,西兰花茎中硫苷的含量最低,为9.96μmol/100 g DW,异硫氰酸盐的含量达到最高,为291.06 mg/g DW。不同温度热激处理结果表明,温度为35℃时硫苷的含量最低为26.44μmol/100 g DW,异硫氰酸盐的含量最高,为597.13 mg/g DW。机械切分、适宜浓度的Zn2+、抗坏血酸和适宜的温度、p H有利于硫苷的降解和异硫氰酸盐的生成,而低温抑制硫苷的降解和异硫氰酸盐的生成。 | 李丽倩 韩勇斌 | 2015 | 食品与发酵工业2015，41，3： | 2 |
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