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| 1 | Growth hormone abolishes the negative effects of everolimus on intestinal wound healing显示文摘AIM: To investigate whether the simultaneous treatment with human growth hormone(h GH) abolishes the negative effects of everolimus on anastomotic healing.METHODS: Forty-eight male Sprague-Dawley-rats were randomized to three groups of 16 animals each(Ⅰ: vehicle; Ⅱ: everolimus 3 mg/kg po; Ⅲ: everolimus 3 mg/kg po + h GH 2.5 mg/kg sc). Animals were pretreated with h GH and/or everolimus daily for seven days. Then a standard anastomosis was created in the descending colon and treatment was continued for another seven days. The anastomosis was resected in toto and the bursting pressure was assessed as a mechanical parameter of intestinal healing. Moreover, biochemical(Hydroxyproline, PCNA, MPO, MMP-2 and MMP-9) and histological(cell density, angiogenesis, amount of granulation tissue) parameters of intestinal healing were assessed.RESULTS: Anastomotic bursting pressure was significantly reduced by everolimus and a simultaneous treatment with h GH resulted in considerably higher values(Ⅰ: 134 ± 19 mm Hg, Ⅱ: 85 ± 25 mm Hg, Ⅲ: 114 ± 25 mm Hg; P < 0.05,Ⅰvs Ⅱ; P = 0.09,Ⅰvs Ⅲ and Ⅱ vs Ⅲ) Hydroxyproline concentration was significantly increased by h GH compared to everolimus alone(Ⅰ: 14.9 ± 2.5 μg/mg, Ⅱ: 8.9 ± 3.6 μg/mg, Ⅲ: 11.9 ± 2.8 μg/mg; P < 0.05,?Ⅰvs Ⅱ/Ⅲ and Ⅱ vs Ⅲ). The number of MPO-positive cells was reduced significantly by h GHcompared to everolimus alone(Ⅰ: 10 ± 1 n/mm^2, Ⅱ: 15 ± 3 n/mm^2, Ⅲ: 9 ± 2 n/mm^2; P < 0.05,Ⅰvs Ⅱ and Ⅱ vs Ⅲ), while the number of PCNA-positive cells were increased by h GH(Ⅰ: 28 ± 3 /mm^2, Ⅱ: 12 ± 3 /mm^2, Ⅲ: 26 ± 12 /mm^2; P < 0.05,?Ⅰ?vs Ⅱ and Ⅱ vs Ⅲ). Corresponding to these biochemical findings, HEhistology revealed significantly increased amount of granulation tissue in h GH-treated animals.CONCLUSION: Inhibition of intestinal wound healing by everolimus is partially neutralized by simultaeous treatment with h GH. Both inflammation as well as collagen deposition is influenced by h GH. | Markus Alexander Küper Sebastian Trütschel Jürgen Weinreich Alfred Konigsrainer Stefan Beckert | 2016 | World Journal of Gastroenterology2016，22，17： | 0 |
| 2 | 粒-巨噬细胞集落刺激因子对化疗后大鼠结肠吻合口愈合的影响显示文摘目的:探讨粒-巨噬细胞集落刺激因子对化疗后大鼠结肠吻合口愈合的影响情况。方法:28只Wistar大鼠实施结肠吻合术,术后随机分草酸铂组(A组)和草酸铂+GM-CSF组(B组),每组14只,B组于吻合口两侧浆膜下层注射GM-CSF。术后24小时两组腹腔注射草酸铂25mg/kg。测定吻合口爆破压、羟脯氨酸含量及胶原纤维含量。结果术后第7天二组均出现吻合口漏,分别为3例和2例,差异无统计学意义;二组爆破压值及羟脯氨酸含量分别为:(169.26±4.31)mm Hg、(191.15±1.84)mm Hg;(9.86±0.40)μg/mg、(11.38±0.50)μg/mg;Ⅰ、Ⅲ型胶原纤维含量分别为:(1.08±0.11)p·μm2、(1.43±0.08)p·μm2;(1.06±0.10)p·μm2、(1.34±0.09)p·μm2。A组与B组比较差异有统计学意义(P<0.05)。结论:局部注射GM-CSF可促进结肠吻合口愈合。 | 龙彦 李淼 刘勇 杨阳 | 2015 | 科学大众(智慧教育)2015，，1： | 0 |
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