| 1 | Characterization of QTL for unique agronomic traits of new-plant-type rice varieties using introgression lines of IR64显示文摘To enhance the yield potential of an elite indica rice cultivar,an introgression(BC_3-derived) line of IR64,YTH288,was developed using a new-plant-type cultivar,IR66215-44-2-3,as a donor parent.YTH288 has agronomically valuable characteristics such as large panicles,few unproductive tillers,and large leaves inherited from NPT.To identify the genetic basis of these traits,we used 167 F_2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits:days to heading(DTH),culm length(CL),flag leaf length(FLL),flag leaf width(FLW),and filled spikelet number per panicle(FSN).Six putative QTL were detected:four on chromosome4(for CL,FLL,FLW,and FSN) and two on chromosome 2(for DTH and FLL).All QTL with the IR66215-44-2-3 allele,except that for FLL on chromosome 2,had positive effects on each trait.To confirm the effects of these putative QTL,we developed NILs with the IR64 genetic background by marker-assisted selection.We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4.Additionally,four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropic To enhance the yield potential of an elite indica rice cultivar, an introgression(BC3-derived) line of IR64, YTH288, was developed using a new-plant-type cultivar,IR66215-44-2-3, as a donor parent. YTH288 has agronomically valuable characteristics such as large panicles, few unproductive tillers, and large leaves inherited from NPT.To identify the genetic basis of these traits, we used 167 F2 plants derived from a cross between IR64 and YTH288 to conduct QTL analysis for five agronomic traits: days to heading(DTH), culm length(CL), flag leaf length(FLL), flag leaf width(FLW), and filled spikelet number per panicle(FSN). Six putative QTL were detected: four on chromosome4(for CL, FLL, FLW, and FSN) and two on chromosome 2(for DTH and FLL). All QTL with the IR66215-44-2-3 allele, except that for FLL on chromosome 2, had positive effects on each trait. To confirm the effects of these putative QTL, we developed NILs with the IR64 genetic background by marker-assisted selection. We observed significant differences in several agronomic traits between IR64 and NILs that carried these QTL on chromosomes2 and 4. Additionally, four IR64-NILs carrying chromosomal segments derived from different NPT varieties on the long arm of chromosome 4 exhibited similar pleiotropiceffects for unique agronomic traits. These NILs can be used as research materials for studying each trait and as breeding materials for yield improvement of indica rice cultivars. | Analiza G.Tagle Daisuke Fujita Leodegario A.Ebron Mary Jeanie Telebanco-Yanoria Kazuhiro Sasaki Tsutomu Ishimaru Yoshimichi Fukuta Nobuya Kobayashi | 2016 | The Crop Journal2016，4，1： | 2 |
| 2 | 水稻抽穗期QTL的遗传定位和效应验证显示文摘水稻抽穗期是一个重要的农艺性状,与产量密切相关。为了探索抽穗期的遗传基础,本研究利用中国香稻和川香29B构建的重组自交系(recombinant inbred lines,RIL)群体,对控制水稻抽穗期的数量性状位点(quantitative trait loci,QTL)进行定位分析。结果显示:2010年和2011年两年共检测到q Hd1a、q Hd1b、q Hd4、q Hd6a、q Hd6b、q Hd6c、q Hd7、q Hd8a、q Hd8b等9个控制抽穗期的QTL,分别位于第1、第4、第6、第7和第8染色体上,贡献率在3.31%~29.64%之间。其中,q Hd1a、q Hd6b、q Hd6c、q Hd7、q Hd8b在两年中均被重复检测到。位于第1染色体上RM3746~RM259区间内的q Hd1a是一个尚未克隆的QTL位点,贡献率为8.21%。针对q Hd1a,本研究采用分子标记辅助选择的方法,构建其近等基因系(near isogenic lines,NIL)群体BC3F2并进行遗传效应分析。与携带纯合川香29B来源的q Hd1a位点的NIL(q Hd1a CX29B)相比,携带纯合中国香稻来源的q Hd1a位点的NIL(q Hd1a ZX)的抽穗期长7 d,差异达到极显著水平。本研究可望为水稻育种的生育期选择提供基础性数据。 | 叶红 李平波 高冠军 张庆路 何予卿 | 2015 | 分子植物育种2015，13，8： | 1 |
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