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| 1 | Updates in the pathophysiological mechanisms of Parkinson's disease: Emerging role of bone marrow mesenchymal stem cells显示文摘AIM: To explore the approaches exerted by mesenchymal stem cells(MSCs) to improve Parkinson's disease(PD) pathophysiology.METHODS: MSCs were harvested from bone marrowof femoral bones of male rats, grown and propagated in culture. Twenty four ovariectomized animals were classified into 3 groups: Group(1) was control, Groups(2) and(3) were subcutaneously administered with rotenone for 14 d after one month of ovariectomy for induction of PD. Then, Group(2) was left untreated, while Group(3) was treated with single intravenous dose of bone marrow derived MSCs(BM-MSCs). SRY gene was assessed by PCR in brain tissue of the female rats. Serum transforming growth factor beta-1(TGF-β1), monocyte chemoattractant protein-1(MCP-1) and brain derived neurotrophic factor(BDNF) levels were assayed by ELISA. Brain dopamine DA level was assayed fluorometrically, while brain tyrosine hydroxylase(TH) and nestin gene expression were detected by semi-quantitative real time PCR. Brain survivin expression was determined by immunohistochemical procedure. Histopathological investigation of brain tissues was also done.RESULTS: BM-MSCs were able to home at the injured brains and elicited significant decrease in serum TGF-β1(489.7 ± 13.0 vs 691.2 ± 8.0, P < 0.05) and MCP-1(89.6 ± 2.0 vs 112.1 ± 1.9, P < 0.05) levels associated with significant increase in serum BDNF(3663 ± 17.8 vs 2905 ± 72.9, P < 0.05) and brain DA(874 ± 15.0 vs 599 ± 9.8, P < 0.05) levels as well as brain TH(1.18 ± 0.004 vs 0.54 ± 0.009, P < 0.05) and nestin(1.29 ± 0.005 vs 0.67 ± 0.006, P < 0.05) genes expression levels. In addition to, producing insignificant increase in the number of positive cells for survivin(293.2 ± 15.9 vs 271.5 ± 15.9, P > 0.05) expression. Finally, the brain sections showed intact histological structure of the striatum as a result of treatment with BM-MSCs. CONCLUSION: The current study sheds light on the therapeutic potential of BM-MSCs against PD pathophysiology via multi-mechanistic actions. | Hanaa H Ahmed Ahmed M Salem Hazem M Atta Emad F Eskandar Abdel Razik H Farrag Mohamed A Ghazy Neveen A Salem Hadeer A Aglan | 2016 | World Journal of Stem Cells2016，8，3： | 9 |
| 2 | 间充质干细胞移植后抑制肝星状细胞活化减轻肝纤维化显示文摘目的 :研究间充质干细胞(mesenchymal stem cell,MSC)移植后对肝纤维化及肝星状细胞(hepatic stellate cells,HSC)活化的抑制作用。方法:Ficoll-Hypaque梯度密度离心法及细胞贴壁培养法分离纯化4周龄SD大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cell,BM-MSC)。取18只SD大鼠,通过腹腔小剂量注射四氯化碳(CCl4)8周,进行肝纤维化造模,在造模4周时,取其中9只作为治疗组,每周经尾静脉移植给予该组每只大鼠6×106个MSC;另外9只作为模型对照组,经尾静脉给予不含MSCs的等量生理盐水;另取9只大鼠,作为正常对照组,仅给予尾静脉等量生理盐水注射,持续4周。自实验开始,每周用全自动生化分析仪测定血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)、白蛋白(ALB)水平,至结束共测定8次。实验终点时通过免疫组化对α平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)、转化生长因子β1(transforming growth factorβ1,TGF-β1)和Ⅰ型胶原蛋白(collagenⅠ,COLⅠ)在肝组织中的定位和表达进行研究;原位灌注和梯度密度离心法分离HSC,326 nm紫外光激发和α-SMA免疫荧光染色法鉴定HSC;q RT-PCR和Western blot检测HSC中α-SMA、TGF-β1基因和蛋白的表达水平。结果:与模型组相比,治疗组经MSC移植后,血清ALT、AST、TBIL水平,肝组织纤维化和炎症程度,肝组织α-SMA、TGF-β1和COLⅠ表达水平,HSC中α-SMA、TGF-β1基因和蛋白表达水平均明显降低。结论:肝纤维化SD大鼠经BM-MSC尾静脉移植治疗后肝功能显著改善、肝纤维化程度减轻,可能与抑制HSC活化有关。 | 施启鹏 郭圆圆 周晗 蔡洁 陈念 李军 章莉莉 | 2015 | 南京医科大学学报(自然科学版)2015，35，7： | 5 |
| 3 | 人脐带间充质干细胞原代培养方法的改良显示文摘目的:探讨体外组织块培养原代人脐带间充质干细胞(human umbilical cord-derived mesenchymal stem cells,hUC-MSCs)的改良方法,并观察其形态,免疫表型,成脂成骨分化等生物学特性。方法:从健康足月儿获得脐带,将血管剥离后,余下的结缔组织剪成小块,分别用传统植块法和改良植块法分离培养原代hUC-MSCs。MTT法检测细胞增殖情况并绘制细胞生长曲线,流式细胞仪检测细胞免疫表型,成脂成骨诱导分化检测其分化潜能,化学染色鉴定诱导分化结果。结果:改良植块法脐带组织贴壁培养3~4 d即可从组织块边缘长出长梭形成纤维样原代细胞,5~6 d后细胞可长满80%,比传统植块法原代细胞培养周期缩短了一半以上时间,细胞传代后流水状或漩涡状生长,MTT法显示细胞增殖能力强,流式细胞仪检测提示CD90,CD105,CD73,CD166,CD29,CD44均阳性表达,阳性率均在95%以上,CD45和HLA-DR均阴性表达,阳性率低于2%;诱导分化实验及化学染色结果证实,该细胞具有成脂和成骨多向分化潜能。结论:应用与酶消化法相结合的改良植块法可以获得hUC-MSCs,比传统植块法缩短了原代培养周期,提高了细胞培养效率,所获得细胞贴壁生长,增殖能力强,表达间充质干细胞相关免疫表型,具有多向分化潜能,可在科研和临床应用中作为种子细胞来源。 | 李小战 马红 许辉 李淑 李遇梅 | 2014 | 江苏大学学报(医学版)2014，24，5： | 2 |
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