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| 1 | siRNA沉默FANCD2基因增加肺癌A549/DDP细胞对顺铂化疗的敏感性显示文摘目的:研究siRNA沉默FANCD2基因后人肺腺癌A549/DDP耐药细胞株对顺铂(DDP)耐药性的变化。方法:合成针对FANCD2基因的siRNA(FANCD2-siRNA),采用脂质体将其分别转染肺癌A549细胞株和A549/DDP耐药细胞株。CCK-8法测定经DDP处理的A549和A549/DDP细胞株转染siRNA前后的细胞增殖率变化;免疫印迹法测定2种细胞株转染siRNA前后FANCD2蛋白单泛素化水平;免疫荧光测定胞核中FANCD2蛋白核聚小体的形成。结果:经DDP处理的A549/DDP细胞增殖率,FANCD2蛋白单泛素化水平及核聚小体形成程度明显高于A549细胞。转染FANCD2-siRNA后,A549和A549/DDP细胞的FANCD2蛋白单泛素化水平和核聚小体形成明显降低,细胞增殖率显著下降。结论:应用siRNA转染技术沉默FANCD2基因可抑制FA/BRCA途径的DNA修复功能,从而增加肺癌细胞对DDP的敏感性,部分逆转耐药肺癌细胞株对DDP的耐药性。 | 姜贺果 戴春华 李坚 陈永昌 蓝婷 伍敏 | 2014 | 江苏大学学报(医学版)2014，24，3： | 3 |
| 2 | Genomic alterations in pancreatic cancer and their relevance to therapy显示文摘Pancreatic cancer is a highly lethal cancer type, for which there are few viable therapeutic options. But, with the advance of sequencing technologies for global genomic analysis, the landscape of genomic alterations in pancreatic cancer is becoming increasingly well understood. In this review, we summarize current knowledge of genomic alterations in 12 core signaling pathways or cellular processes in pancreatic ductal adenocarcinoma, which is the most common type of malignancy in the pancreas, including four commonly mutated genes and many other genes that are mutated at low frequencies. We also describe the potential implications of these genomic alterations for development of novel therapeutic approaches in the context of personalized medicine. | Erina Takai Shinichi Yachida | 2015 | World Journal of Gastrointestinal Oncology2015，7，10： | 2 |
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