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| 1 | 小鼠血管平滑肌细胞原代培养及体外钙化模型的制备显示文摘目的:建立一种简单、高效的小鼠主动脉血管平滑肌细胞(vascular smooth muscle cells,VSMCs)原代培养方法及体外钙化模型。方法:采用改良的组织贴块法培养小鼠VSMCs,经免疫荧光染色法鉴定后,将细胞随机分为对照组和钙化组。采用von kossa染色及比色法检测两组细胞内钙含量。结果:培养3~5 d时,可见细胞从组织块边缘爬出,7~10 d后细胞融合成片;免疫荧光染色显示胞质表达特异性α-肌动蛋白;VSMCs培养至第2代细胞纯度达95%;与对照组相比,钙化组细胞的钙含量、碱性磷酸酶(ALP)活性明显增加(P均<0.05)。结论:改良的组织贴块法可获得高纯度的小鼠VSMCs;β-甘油磷酸钠在体外可有效诱导其钙化。 | 徐丽华 严金川 伍超 逯朝阳 王中群 袁伟 | 2014 | 江苏大学学报(医学版)2014，24，2： | 4 |
| 2 | Arterial calcification: Finger-pointing at resident and circulating stem cells显示文摘The term 'Stammzelle'(stem cells) originally appeared in 1868 in the works of Ernst Haeckel who used it to describe the ancestor unicellular organism from which he presumed all multicellular organisms evolved. Since then stem cells have been studied in a wide spectrum of normal and pathological conditions; it is remarkable to note that ectopic arterial calcification was considered a passive deposit of calcium since its original discovering in 1877; in the last decades, resident and circulating stem cells were imaged to drive arterial calcification through chondro-osteogenic differentiation thus opening the idea that an active mechanism could be at the basis of the process that clinically shows a Janus effect: calcifications either lead to the stabilization or rupture of the atherosclerotic plaques. A review of the literature underlines that 130 years after stem cell discovery, antigenic markers of stem cells are still debated and the identification of the osteoprogenitor phenotype is even more elusive due to tissue degradation occurring at processing and manipulation. It is necessary to find a consensus to perform comparable studies that implies phenotypic recognition of stem cells antigens. A hypothesis is based on the singular morphology and amitotic mechanism of division of osteoclasts: it constitutes the opening to a new approach on osteoprogenitors markers and recognition. Our aim was to highlight all the present evidences of the active calcification process, summarize the different cellular types involved, and discuss a novel approach to discover osteoprogenitor phenotypes in arterial wall. | Francesco Vasuri Silvia Fittipaldi Gianandrea Pasquinelli | 2014 | World Journal of Stem Cells2014，6，5： | 0 |
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