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| 1 | 泡盛曲霉发酵麦麸过程中酚类物质含量的变化与三种酶活性的相关性显示文摘本研究以小麦麦麸为原料,分别采用3株泡盛曲霉(分别为GIM3.266,GIM3.4,GIM3.5)进行连续8天的固态发酵,每天分别提取发酵过程中小麦麦麸中不同存在形态酚类物质并测定其含量,同时,每天测定3株泡盛曲霉在发酵过程中木聚糖酶、纤维素酶以及阿魏酸酯酶的活性变化。结果表明,与未经发酵的麦麸相比,经泡盛曲霉发酵后的麦麸游离型酚类物质的含量显著增加,总酚含量可高达11561.30μg/g麸皮,比发酵前的麸皮总酚提高了169%;另外,酶活力的结果表明:纤维素酶活力在发酵前三天显著增大,其活力最高可达156.70 U,木聚糖酶活力随总酚释放量的增加而增加,最高可达34123.00 U,阿魏酸酯酶活力在发酵中后期达到顶峰,最高可达2642.60 U。总体而言,麦麸发酵过程中酚类物质的释放量与木聚糖酶和阿魏酸酯酶的酶活力呈正相关,说明发酵后麦麸中酚类物质提取量的显著增加与这两种酶密切相关,这两种酶活力高的泡盛曲霉菌株,更有利于释放麦麸中的酚类物质。 | 杜小燕 吴晖 赵超敏 闵甜 唐语谦 李晓凤 赖富饶 | 2015 | 现代食品科技2015，31，4： | 9 |
| 2 | 植物提取物在家禽抗氧化上的应用显示文摘植物提取物作为安全、有效、无残留的饲料添加剂已经得到越来越多的使用与重视,原因是其具有抗氧化、抗菌、促生长等多种对养殖业有益的作用。在养禽行业,鸡肉、鸡蛋等禽产品在贮藏过程中极易受到氧化而发生变质,而植物提取物可以有效地延长禽产品的贮藏时间。本文主要综述了植物提取物在肉鸡和蛋鸡上的抗氧化作用以及其体内和体外的作用机制,为以后植物提取物在家禽抗氧化上的应用研究提供依据。 | 李文强 马春阳 轩振 李鸿博 史东辉 陈俊锋 | 2014 | 现代畜牧兽医2014，，11： | 6 |
| 3 | Chemical analysis,antimicrobial and anti-oxidative properties of Daucus gracilis essential oil and its mechanism of action显示文摘Objective:To evaluate the essential oils(EO) composition,antimicrobial and antioxidant power of a local plant,Daucus gracilis(D.gracilis).Methods:The aerial parts of D.gracilis were subjected to hydro distillation by a Clevenger apparatus type to obtain the EO which had been analyzed by gas chromatography and gas chromatography coupled with mass spectrometry,and screened for antimicrobial activity against five bacteria and three fungi by agar diffusion method.The mechanism of action of the EO was determined on the susceptible strains by both of time kill assay and lysis experience.The minimal inhibitory concentrations were determined by agar macrodilution and micro-dilution methods.Anti-oxidative properties of the EO were also studied by free diphenyl-2-picrylhydrazyl radical scavenging and reducing power techniques.Results:The EO yielded 0.68(v/w).The chemical analysis presented two dominant constituents which were the elemicin(35.3%) and the geranyl acetate(26.8%).D.gracilis EO inhibited the growth of Bacillus cereus and Proteus mirabilis significantly with minimal inhibitory concentrations of 17.15 μg/mL by the agar dilution method and57.05 μg/mL and 114.1 μg/mL,respectively by liquid micro-dilution.A remarkable decrease in a survival rate as well as in the absorbance in 260 nm was recorded,which suggested that the cytoplasm membrane was one of the targets of the EO.The EO showed,also,important anti-oxidative effects with an IC50 of 0.002 mg/mL and a dosedependent reducing power.Conclusions:D.gracilis EO showed potent antimicrobial and anti-oxidative activities and had acted on the cytoplasm membrane.These activities could be exploited in the food industry for food preservation. | Meriem El Kolli Hocine Laouer Hayet El Kolli Salah Akkal Farida Sahli | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，1： | 2 |
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