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| 1 | Antibiotic susceptibility profiling and virulence potential of Campylobacter jejuni isolates from different sources in Pakistan显示文摘Objective:To determine antibiotic resistance patterns and virulence potential of Campylobacter jejuni(C.jejuni) isolates from clinical human diarrheal infections,cattle and healthy broilers.Methods:Antibiotic sensitivity patterns of C.jejuni isolates were determined by Kirby Bauer Disc Diffusion assay.These isolates were then subjected to virulence profiling for the detection of map A(membrane-associated protein).cadF(fibronectin binding protein).wlaN(beta-1.3-galaclosyltransferase) and neu AB(sialic acid biosynthesis gene).Further C.jejuni isolates were grouped by random amplification of polymorphic DNA(RAPD) profiling.Results:A total of436 samples from poultry(n=88).cattle(n=216) and humans(n=132) from different locations were collected.Results revealed percentage of C.jejuni isolates were 35.2%(31/88).25.0%(54/216) and 11.3%(15/132) among poultry,cattle and clinical human samples respectively.Antibiotic susceptibility results showed that similar resistance patterns to cephalothin was ie.87.0%,87.1%and 89%among humans,poultry and cattle respectively,followed by sulfamethoxazolc+trimcthoprim 40.0%,38.7%and 31.0%in humans,poultry and cattle and Ampicillin 40%,32%and 20%in humans,poultry and cattle respectively.Beta-lactamase activity was detected in 40.00%humans.20.37%cattle and 32.25%in poultry C.jejuni isolates.CadF and mapA were present in all poultry,cattle and human C jejuni isolates.wlauN was not detected in any isolate and neu AB was found in 9/31(36%) poultry isolates.RAPD profiling results suggested high diversity of C.jejuni isolates.Conclusions:Detection of multidrug resistant C.jejuni strains from poultry and cattle is alarming as they can be potential hazard to humans.Moreover,predominant association of virulence factors,cadF and map A(100%each) in C.jejuni isolates from all sources and neuAB(36%) with poultry isolates suggest the potential source of transmission of diverse types of C.jejuni to humans. | Fariha Masood Siddiqui Muhammad Akram Nighat Nourccn Zobia Noreen Habib Bokhari | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，3： | 2 |
| 2 | 家禽养殖过程中弯曲菌流行病学的研究进展显示文摘弯曲菌作为一种人兽共患的食源性病原菌,是引起全球范围内人类细菌性胃肠炎的主要病原菌。家禽是弯曲菌的自然宿主,在弯曲菌的流行中扮演着非常重要的角色。养殖过程中家禽感染弯曲菌后不表现出任何临床症状,但可持续向外界排菌,引起人类及其他动物感染发病,造成了严重的健康危害和经济损失。因此,控制或清除家禽弯曲菌是预防和控制人类弯曲菌病的重要前提。而在家禽养殖过程中开展弯曲菌的流行性监测工作对预防和控制弯曲菌具有重要意义。本文旨在对近年来家禽养殖过程中弯曲菌的流行现状、感染与传播及分子溯源技术等研究进展进行综述,以期为今后农场中家禽弯曲菌的流行和防控提供数据支持。 | 阴甜甜 黄金林 焦新安 | 2015 | 食品安全质量检测学报2015，6，12： | 0 |
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