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| 1 | Anti-proliferation and radiosensitization effects of chitooligosaccharides on human lung cancer line Hep G2显示文摘Objective: To observe the anti-proliferation and radiosensitization effect of chitooligosaccharides(COS) on human lung cancer cell line Hep G2. Methods: CCK-8 assay was employed to obtain the inhibition ratio of COS on Hep G2 cells at 24 h after treatment. The clonogenic assay was used to analyze the cell viability of RAY group and RAY+COS group with X-ray of 0, 1, 2, 4, 6 and 8 Gy, and the cell survival curve was used to analyze the sensitization ratio of COS. Flow cytometry was employed to detect cell cycle and apoptosis rate in control group, RAY group and RAY+COS group after 24 h treatment. Results: COS inhibited the proliferation of Hep G2 cells, and the inhibition rate positively correlated with the concentration of COS. The cell viability decreased with increasing exposure dose in RAY group and RAY+COS group. The cell viabilities of RAY+COS group were lower than those of RAY group at the dose of 4, 6 and 8 Gy(P<0.05), and the sensitization ratio of COS was 1.19. There were higher percentage at G2/M phase and apoptosis rate, and lower percentage at S phase in RAY+COS group versus the other two groups(P<0.01). Conclusions: COS can inhibit the proliferation of Hep G2 cells, and enhance the radiosensitization of Hep G2 cells, induce apoptosis and G2/M phase arrest. | Fu-Shi Han Bo-Han Cui Xiao-Fang You Yan-Fen Xing Xi-Wen Sun | 2015 | Asian Pacific Journal of Tropical Medicine2015，8，9： | 3 |
| 2 | 藻蓝蛋白/羧甲基壳聚糖纳米球的制备及其对人宫颈癌HeLa细胞增殖的抑制显示文摘目的:制备新型负载藻蓝蛋白(C-phycocyanin,C-PC)的羧甲基壳聚糖(carboxymethyl chitosan,CMC)纳米微球(nanoparticle,NP),探讨藻蓝蛋白羧甲基壳聚糖纳米微球(C-PC/CMCNP)对人宫颈癌He La细胞生长的影响及其分子机制。方法:采用正交分析方法,以CMC和C-PC的质量比、CMC浓度、Ca Cl2浓度为考察因素,通过检测C-PC/CMCNP的粒径和CMC的包封率,优选制备C-PC/CMCNP的最佳条件,并制备C-PC/CMCNP。CCK-8法检测C-PC、CMC和C-PC/CMCNP对He La细胞增殖的影响,流式细胞术检测He La细胞凋亡情况,Westren blotting检测He La细胞中caspase-3蛋白的表达。结果:CMC与C-PC的质量比为1∶2、CMC质量浓度为1 mg/ml,Ca Cl2质量浓度为1 mg/ml为最佳制备条件,最终制备的C-PC/CMCNP的平均粒径为(118.4±2.07)nm,并具有较高的包封率(63.2%),其缓释12 h的累积缓释率超过60%。C-PC、CMC和C-PC/CMCNP均能抑制He La细胞的增殖,诱导细胞凋亡,并促进caspase-3蛋白的表达,但C-PC/CMCNP的作用效果最为显著。结论:优化制备条件得到具有高效缓释性能的C-PC/CMCNP,其对He La细胞显著的抑制作用可能是通过促进caspase-3蛋白的表达进而诱导肿瘤细胞凋亡来实现的。 | 吕丛仪 李冰 杨鹏 | 2015 | 中国肿瘤生物治疗杂志2015，22，1： | 1 |
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