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| 1 | OSP-1启动子在猪卵巢颗粒细胞中的转录活性研究显示文摘为获得能在猪卵巢颗粒细胞中高效表达的启动子,本文检测了大鼠卵巢特异性启动子OSP-1(Ovarian specific promoter)在猪卵巢颗粒细胞中的转录活性。参考大鼠OSP-1启动子序列扩增大鼠OSP-1片段,将其定向克隆到pc DNA3.1(+)中替代CMV启动子,并在其下游插入Zs GREEN基因片段,构建真核表达载体p OSP-1-Zs GREEN;重组质粒在脂质体LipofectamineTM2000的介导下转染不同细胞,在荧光显微镜下观察Zs GREEN表达情况,Realtime-RT-PCR方法检测该启动子在不同细胞中启动Zs GREEN基因表达的活性。结果表明:经克隆测序,得到了465bp OSP-1启动子序列,重组质粒转染卵巢颗粒细胞、Hela细胞可以观察到绿色荧光。Realtime-RT-PCR结果显示:Zs GREEN基因在卵巢颗粒细胞、Hela细胞中高表达,在肌细胞中表达量极低;说明OSP-1启动子可驱动外源基因在猪卵巢颗粒细胞中高效表达,可为构建可用于猪卵巢颗粒细胞的表达载体提供了启动子。 | 徐梦思 黄涛 杨飞 刘丽娟 鲁慧文 翟腾蛟 陈亚楠 | 2015 | 石河子大学学报(自然科学版)2015，33，3： | 0 |
| 2 | Effects of Bcl-2 Gene Interference on the Apoptosis,Proliferation and Progesterone Secretion of Goose Follicular Granulosa Cells显示文摘Based on published sequences for chicken Bcl-2,three siRNAs(small interfering RNA)were designed,and expression vectors were constructed and transfected into goose granulosa cells cultured in vitro.Bcl-2 protein,apoptosis and proliferation of granulosa cells,48 h after the transf ection,were analyzed by flow cytometry,and progesterone(P)secreted into the culture medium was measured by radioimmunoassay.In addition,apoptosis and Bcl-2 protein level were assessed in untreated granulosa cells from the four largest preovulatory follicles(F1F4),the smallest preovulatory follicles(SPF),small yellow follicles(SYF)and atretic follicles.The highest level of Bcl-2 protein was observed in granulosa cells from SPF,and levels in cells from healthy follicles were significantly higher than those of atretic follicles(P<0.05).Bcl-2 protein levels in cells subjected to RNAi were significantly lower than those of controls(P<0.05),while apoptosis indices(AI),proliferation indices(PI)and P secretion in the RNAi treatments were higher than those of controls(P<0.05). | CHEN Xiu-ping CHEN Feng-jian JIANG Xun-ping LI Zhao-guo DING Jia-tong | 2009 | 畜牧兽医学报2009，40，S1： | 0 |
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