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| 1 | 山羊y^+L碱性氨基酸转运系统转录水平表达的研究显示文摘旨在研究山羊y+L碱性氨基酸转运系统中4F2hc、y+LAT1和y+LAT2mRNA表达的组织特异性及其在不同年龄山羊小肠中的发育性表达规律。试验选取健康、体重相近的1日龄、6月龄、8月龄、10月龄、12月龄陕北白绒山羊各3只,共15只,屠宰后采集心、肝、肾、大脑、背最长肌、体侧皮肤、瘤胃、十二指肠、空肠、回肠和结肠组织,利用实时荧光定量PCR技术检测4F2hc、y+LAT1和y+LAT2mRNA在1日龄山羊上述组织和全部山羊十二指肠、空肠和回肠的表达情况。结果表明,(1)4F2hc、y+LAT1和y+LAT2mRNA在1日龄山羊多种组织中均有表达,且有一定的组织特异性。其中,4F2hc mRNA在皮肤表达量最高,y+LAT1 mRNA在回肠表达量最高,y+LAT2mRNA在大脑和回肠表达量最高。(2)4F2hc mRNA在山羊十二指肠、空肠和回肠表达量均以1日龄山羊为最高,其他月龄山羊相同肠段之间表达量无显著差异(P>0.05);y+LAT1mRNA在十二指肠、空肠和回肠表达量随山羊年龄的增加均呈现逐渐降低的趋势;y+LAT2mRNA在十二指肠、空肠和回肠表达量随山羊年龄的增加呈现先升高后降低的趋势,其在10月龄山羊表达量最高;y+LAT2mRNA在回肠表达量以1日龄山羊为最低,其他月龄山羊之间差异不显著(P>0.05)。结果说明,山羊y+L碱性氨基酸转运系统的主要转运部位为小肠;该系统中轻链y+LAT1和重链4F2hc构成的异二聚体转运蛋白可能发挥更为主要的转运作用;4F2hc和y+LAT2基因可能分别在山羊皮肤和大脑中发挥重要的生理功能;4F2hc、y+LAT1和y+LAT2基因在山羊小肠中受肠段和发育阶段的影响,具有不同的发育性表达规律。 | 周英昊 高晔 刘林丽 曾洁 杨雨鑫 张恩平 陈玉林 | 2015 | 畜牧兽医学报2015，46，8： | 4 |
| 2 | Effect of Xylanase on mRNA Expression of Cationic Amino Acid Transporters in Intestines of Broilers显示文摘480 healthy 1-day-old male yellow-feathered chickens were selected and assigned randomly into groups A and B,each having 6 pens with 40 birds per pen.The birds in group A were fed with wheatbased diet and group B with wheat-based diet supplemented with xylanase(1.2×l0~4 U/kg diet).On day 16,two birds per replication with average live weight were selected and sacrificed.Tissue samples of jejunum and ileum were collected to detect mRNA expression of cationic amino acid transporters using RT-PCR.The results showed that xylanase significantly increased the abundance of mRNA for rBAT and CAT4 in the intestines of broilers fed with wheat-based diets(P<0.05)and had a tendency to increase the mRNA expression of y^+LAT2 and CAT1 in jejunum(P>0.05),y^+LAT2,CAT1 and CAT4 in ileum(P>0.05).The treatment had no effect on the expression of rBAT mRNA in ileum(P>0.05). | FENG Ding-yuan TAN Hui-Ze WANG Xiu-qi ZOU Shi-geng ZUO Jian-jun HUANG Yan-hua DONG Ze-min ZHANG Chang-ming YE Hui | 2009 | 畜牧兽医学报2009，40，S1： | 0 |
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