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| 1 | 褐黄血蜱的形态学与分子生物学鉴定显示文摘鉴定采自河南信阳、湖南华容、山西太谷刺猬、犬的蜱。借助体视显微镜对蜱样进行形态观察;克隆、分析其COX1与ITS1序列,对比分析同源性并构建系统发生树。形态学鉴定和分子生物学鉴定结果表明,采自河南信阳、湖南华容、山西太谷刺猬、犬体表的蜱均为褐黄血蜱。湖南、河南、山西也成为褐黄血蜱的分布地。 | 严芬 程天印 | 2015 | 中国兽医学报2015，35，6： | 13 |
| 2 | Precise identification of different stages of a tick,Ixodes granulatus Supino,1897(Acari:Ixodidae)显示文摘Objective: To identify different stages of Ixodes granulatus(I. granulatus) based on morphological characters prior to molecular identification which is significant for confirming and identifying the nymphal stages of I. granulatus.Methods: A total of 14 individuals of adult, engorged and nymphal ticks collected from three different localities were examined morphologically using taxonomic keys, followed by PCR using cytochrome oxidase subunit I(COI). Clustering analysis based on COI sequences was carried out by constructing neighbor-joining and maximum parsimony tree to clarify the genetic variation and diversity of local I. granulatus.Results: Based on external morphological characterizations, nine individuals(64.3%)were successfully identified as I. granulatus, while five individuals were recognized only as Ixodes sp. due to lack of morphological characters visible and development during that stage. Molecular analysis of local I. granulatus using COI gene revealed 93%–94% sequence homology from available sequence in Gen Bank and was in concordance with the morphological identification. Furthermore, a low intraspecific variation was observed among the species of I. granulatus collected from different localities(0%–3.7%).Conclusions: These findings demonstrated for the first time the establishment of COI gene for identifying I. granulatus nymphal tick which is of paramount importance to the control of potential tick-borne infections in Malaysia. Moreover, this study provides evidence that a combination of morphology and molecular data was corroborated as an accurate tool for tick identification. | Ernieenor Faraliana Che Lah Salmah Yaakop Mariana Ahamad Ernna George Shukor Md Nor | 2016 | Asian Pacific Journal of Tropical Biomedicine2016，6，7： | 0 |
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