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| 1 | 白菜种子萌发的热抑制现象及其与细胞壁降解酶的关系显示文摘为了探讨白菜种子萌发热抑制的机制,以新收获的白菜种子为材料,研究了种子萌发对不同温度的响应,后熟、冷层积和植物激素处理对种子萌发的作用,以及种子萌发和不同处理与细胞壁降解酶之间的关系。结果表明,当萌发温度≥20℃时,种子的萌发率显著降低,热抑制的种子不表现次生休眠。后熟、层积和GA3处理能有效地降低种子萌发的热抑制;相反,ABA处理则增强种子萌发的热抑制。种子萌发过程中内切–β–甘露聚糖酶、β–甘露糖苷酶和α–半乳糖苷酶的活性增加。与新收获的种子相比,后熟、层积和GA3处理增加上述3种酶的活性;ABA处理增加内切–β–甘露聚糖酶的活性,但不影响β–甘露糖苷酶和α–半乳糖苷酶的活性。在不同温度下吸胀48 h的种子的内切–β–甘露聚糖酶和α–半乳糖苷酶的活性变化与随后种子的萌发没有直接关系。说明新收获的白菜种子具有明显的萌发热抑制,这种特性能被后熟、层积和GA3处理以增强细胞壁降解酶活性的方式有效地解除。 | 宋顺华 宋松泉 吴萍 孟淑春 邢宝田 | 2014 | 园艺学报2014，41，6： | 5 |
| 2 | Identification and functional analysis of miRNAs in developing kernels of a viviparous mutant in maize显示文摘Given the important roles of miRNAs in post-transcriptional gene regulation, identification of differentially expressed miRNAs will facilitate the elucidation of molecular mechanisms underlying kernel development. In this study, we constructed a small RNA library to comprehensively represent the full complement of individual small RNAs and to characterize miRNA expression profiles in pooled ears of maize(Zea mays L.) at 10, 15,20, 22, 25 and 30 days after pollination(DAP). At least 21 miRNAs were differentially expressed. The differential expression of three of these miRNAs, i.e., miR528a, miR167a and miR160b, at each stage was verified by qRT-PCR. The results indicated that these miRNAs might be involved in kernel development. In addition, the predicted functions of target genes indicated that most of the target genes are involved in signal transduction and cell communication pathways, particularly the auxin signaling pathway. The expression of candidate germination-associated miRNAs was analyzed by hybridization to a maize genome microarray, and revealed differential expression of genes involved in plant hormone signaling pathways. This finding suggests that phytohormones play a critical role in the development of maize kernels. We found that in combination with other miRNAs, miR528a regulated a putative laccase, a Ring-H2 zinc finger protein and a MADS box-like protein, whereas miR167a and miR160b regulated multiple target genes,including ARF(auxin response factor), a member of the B3 transcription factor family. All three miRNAs are important for ear germination, development and physiology. The small RNA transcriptomes and mRNA obtained in this study will help us gain a betterunderstanding of the expression and function of small RNAs in the development of maize kernel. | Haiping Ding Jian Gao Mao Luo Hua Peng Haijian Lin Guangsheng Yuan Yaou Shen Maojun Zhao Guangtang Pan Zhiming Zhang | 2013 | The Crop Journal2013，1，2： | 0 |
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