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1Introduction of rol Genes into Cotton (Gossypium hirsutum L.) Genome and Effects of Transgene Expression on the Plant Development显示文摘The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.LIUHai-yan YANGYe-hua WUZheng-bin WANGXue-kui YAOMing-jin 2004Agricultural Sciences in China2004,3,10:3
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