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| 1 | Biogenesis of antibacterial silver nanoparticles using the endophytic bacterium Bacillus cereus isolated from Garcinia xanthochymus显示文摘Objective:To synthesize the ecofriendly nanoparticles,which is viewed as an alternative to the chemical method which initiated the use of microbes like bacteria and fungi in their synthesis.Methods:The current study uses the endophytic bacterium Bacillus cereus isolated from the Garcinia xanthochymus to synthesize the silver nanoparticles(AgNPs).The AgNPs were synthesized by reduction of silver nitrate solution by the endophytic bacterium after incubation for 3-5 d at room temperature.The synthesis was initially observed by colour change from pale white to brown which was confirmed by UV-Vis spectroscopy.The AgNPs were further characterized using FTIR,SEM-EDX and TEM analyses.Results:The synthesized nanoparticles were found to he spherical with the size in the range of 20-40 nm which showed a slight aggregation.The energy-dispersive spectra of the nanoparticle dispersion confirmed the presence of elemental silver.The AgNPs were found to have antibacterial activity against a few pathogenic bacteria like Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus,Salmonella typhi and Klebsiella pneumoniae.Conclusions:The endophytic bacteria identified as Bacillus cereus was able to synthesize silver nanoparticles with potential antibacterial activity. | Swetha Sunkar C Valli Nachiyar | 2012 | Asian Pacific Journal of Tropical Biomedicine2012，2，12： | 5 |
| 2 | Antimicrobial activity of latex silver nanoparticles using Calotropis procera显示文摘Objective:To synthesize silver nanoparticles(AgNPs) by green methods using scrum latex of Calotropis procera at 80 ℃ and evaluate them against bacteria,dermatophytes and phytopathogenic fungi comparing with the activity of untreated latex.Methods:The synthesis of AgNPs was performed by mixing 3%latex scrum extract with the same volume of silver nitrate(2 mmol/L) solution in round flask and heating in water bath at80 ℃.Characterization of silver particles were determined using UV-vis spectrophotometer,transmission electron microscopy(TEM),X-ray diffraction and Fourier transform infrared spectroscopy.The antimicrobial activity of the green synthesized AgNPs was determined against bacteria,dermatophytes and phytopathogenic fungi and compared to the crude untreated latex by agar-well diffusion methods.Results:Biosynthesis of latex silver nanoparticles was successfully obtained by green method.The formation of AgNPs has been confirmed by UV-vis,TEM microscopy.X-ray diffraction and Fourier transform infrared spectroscopy.TEM analysis showed that synthesized AgNPs are highly stable spherical shaped particles,well dispersed with a diameter ranged from 4 nm up to 25 nm and an average size of 12.33 nm.AgNPs showed strong antibacterial activity against Gram-negative bacteria(Escherichia coli,Pseudomonas aeruginosa and Serratia sp.) and antifungal activity against Trichophyton rubrum,Candida albicans and Aspergillus terreus.Conclusions:It can be concluded that serum latex of Calotropis pmcera was found to display strong potential for the synthesis of AgNPs as antimicrobial agents through rapid reduction of silver ions(Ag^+ to Ag^0).The green synthesized AgNPs were found to show higher antimicrobial efficacy than crude latex. | Nadia Hussein Mohamed Mady Ahmed Ismail Wael Moustfa Abdel-Mageed Ahmed Abdelfattah Mohamed Shoreit | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，11： | 1 |
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