| 1 | Anti-inflammatory activity and qualitative analysis of different extracts of Maytenus obscura(A.Rich.) Cuf.by high performance thin layer chromatography method显示文摘Objective:To perform aqueous elhanol soluble fraction(AESK) and dichloromethane extract of aerial parts of Maytenus obscura(A.Rich.) Cuf.using high performance thin layer chromatography(HPTLC) and to test anti-inflammatory activity of these extracts.Methods:HPTLC studies were carried out using CAM At;HPTLC system pquipped with Linomat IV applicator,TLC scanner 3.Reprostar 3.CAMAG ADC 2 and WIN CAT5-4 software were used.The anti-inflammatory activity was tested by injecting different groups of rats(6 each) with formalin in hind paw and measuring the edema volume before and 1h later formalin injection.Control group received saline i.p.The extracts treatment was injected i.p.in doses of 100 and 200 mg/kg 1h before formalin administration.Indomethaein(30 mg/kg) was used as standard.Results:The results of preliminary phytochemical studies confirmed the presence of protein,lipid,carbohydrate,phenol,flavonoid.saponin,triterpenoid,alkaloid and anthraquinonc in both extracts.Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene:ethyacetate:glacial acetic acid(5:3:0.2,v/v/v) as mobile phase.HPTLC.finger printing of AESF revealed major eight peaks with R_f values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with R_f values in the range of 0.12 to 0.76.The purity of sample was confirmed by comparing the absorption spectra at start,middle and end position of the hand.Treatment of rats(i.p.) with AESF and dirhloromethane in doses of 100 and 200 mg/kg inhibited singnificantly(P<0.05.n=6) formalin-induced inflammation by 50%.55.9%,45.5%,and51.4%.respectively.Conclusions:HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts.These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts.The anti-inflammatory activity of both extracts also revealed the medicinal importance of these extracts.The plant can be further explored for the isolation of phyloconstiluents having anti-inflammatory activity. | Mohamed F.Alajmi Perwez Alam | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，2： | 0 |
| 2 | Chromatographic finger print analysis of anti-inflammatory active extract fractions of aerial parts of Tribulus terrestris by HPTLC technique显示文摘Objective:To develop HPTLC fingerprint profile of anti-inflammatory active extract fractions of Tribulus terrestris(family Zygophyllaceae).Methods:The anti-inflammatory activity was tested for the methanol and its fractions(chloroform,ethyl acetate,n-butanol and aqueous)and chloroform extract of Tribulus terrestris(aerial parts)by injecting different groups of rats(6 each)with carrageenan in hind paw and measuring the edema volume before and 1,2 and 3 h after carrageenan injection.Control group received saline i.p.The extracts treatment was injected i.p.in doses of 200 mg/kg 1 h before carrageenan administration.Indomethacin(30 mg/kg)was used as standard.HPTLC studies were carried out using CAMAG HPTLC system equipped with Linomat IV applicator.TLC scanner 3.Reprostar 3,CAMAG ADC 2 and WIN CATS-4 software for the active fractions of chloroform fraction of methanol extract.Results:The methanol extract showed good antiedematous effect with percentage of inhibition more than 72%,indicating its ability to inhibit the inflammatory mediators.The methanol extract was re-dissolved in 100 mL of distilled water and fractionated with chloroform,ethyl acetate and n-butanol.The four fractions(chloroform,ethyl acetate,n-butanol and aqueous)were subjected to anti-inflammatory activity.Chloroform fraction showed good anti-inflammatory activity at dose of 200 mg/kg.Chloroform fraction was then subjected to normal phase silica gel column chromatography and eluted with petroleum ether-chloroform,chloroform-ethyl acetate mixtures of increasing polarity which produced 15 fractions(F1-F15).Only fractions F1,F2,F4,F5,F7,F9,F11 and F14 were found to be active,hence these were analyzed with HPTLC to develop their finger print profile.These fractions showed different spots with different R_f values.Conclusions:The different chloroform fractions F1,F2,F4,F5,F7,F9,F11 and F14 revealed 4,7,7,8,9,7,7 and 6 major spots,respectively.The results obtained in this experiment strongly support and validate the traditional uses of this Sudanese medicinal plant. | Mona Salih Mohammed Mohamed Fahad Alajmi Perwez Alam Hassan Subki Khalid Abelkhalig Muddathir Mahmoud Wadah Jamal Ahmed | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，3： | 0 |
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