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| 1 | 碱性成纤维细胞生长因子转染骨髓间充质干细胞移植治疗肺动脉高压显示文摘背景:目前正在兴起的干细胞治疗技术及基因修饰技术有可能在肺动脉高压治疗中获得独特疗效。目的:探讨携带碱性成纤维细胞生长因子基因的骨髓间充质干细胞移植对肺动脉高压大鼠血流动力学水平的影响。方法:选择3周龄SD雄性大鼠,于体外进行骨髓间充质干细胞培养及纯化,在腺病毒介导下实施基因转染,使碱性成纤维细胞生长因子基因成功导入骨髓间充质干细胞。将60只SD大鼠给予野百合碱腹腔注射(50 mg/kg)进行肺动脉高压模型复制,随机分成3组,其中肺动脉高压组于颈内静脉移植1 m L的L-DMEM培养基,骨髓间充质干细胞组于颈内静脉移植1 m L空腺病毒载体转染的骨髓间充质干细胞悬液,碱性成纤维细胞生长因子组于颈内静脉移植1 m L腺病毒介导下碱性成纤维细胞生长因子转染骨髓间充质干细胞悬液。结果与结论:经3周治疗后,各组大鼠动脉血压差异无显著性意义(P>0.05);其中碱性成纤维细胞生长因子组大鼠的肺动脉收缩期压力和平均肺动脉压明显低于肺动脉高压组、骨髓间充质干细胞组,差异有显著性意义(P<0.05);各组大鼠右心室的肥大指数比较差异无显著性意义(P>0.05);碱性成纤维细胞生长因子组大鼠血浆内皮素1水平明显低于肺动脉高压组、骨髓间充质干细胞组,差异有显著性意义(P<0.05)。结果显示携带碱性成纤维细胞生长因子的骨髓间充质干细胞移植,能够改善肺动脉高压大鼠肺血管的血流动力学水平,保护机体血管的内皮细胞,并拮抗血管的收缩。 | 宋燕峰 曹洁 | 2015 | 中国组织工程研究2015，19，6： | 4 |
| 2 | WJSC 6^(th) Anniversary Special Issues(2):Mesenchymal stem cells “Ins” and “Outs” of mesenchymal stem cell osteogenesis in regenerative medicine显示文摘Repair and regeneration of bone requires mesenchymal stem cells that by self-renewal,are able to generate a critical mass of cells with the ability to differentiate into osteoblasts that can produce bone protein matrix(osteoid)and enable its mineralization.The number of human mesenchymal stem cells(hMSCs)diminishes with age and ex vivo replication of hMSCs has limited potential.While propagating hMSCs under hypoxic conditions may maintain their ability to self-renew,the strategy of using human telomerase reverse transcriptase(hTERT)to allow for hMSCs to prolong their replicative lifespan is an attractive means of ensuring a critical mass of cells with the potential to differentiate into various mesodermal structural tissues including bone.However,this strategy must be tempered by the oncogenic potential of TERT-transformed cells,or their ability to enhance already established cancers,the unknown differentiating potential of high population doubling hMSCs and the source of hMSCs(e.g.,bone marrow,adipose-derived,muscle-derived,umbilical cord blood,etc.)that may provide peculiarities to self-renewal,differentiation,and physiologic function that may differ from non-transformed native cells.Tissue engineering approaches to use hMSCs to repair bone defects utilize the growth of hMSCs on three-dimensional scaffolds that can either be a base on which hMSCs can attach and grow or as a means of sequestering growth factors to assist in the chemoattraction and differentiation of native hMSCs.The use of whole native extracellular matrix(ECM)produced by hMSCs,rather than individual ECM components,appear to be advantageous in not only being utilized as a three-dimensional attachment base but also in appropriate orientation of cells and their differentiation through the growth factors that native ECM harbor or in simulating growth factor motifs.The origin of native ECM,whether from hMSCs from young or old individuals is a critical factor in'rejuvenating'hMSCs from older individuals grown on ECM from younger individuals. | Dean T Yamaguchi | 2014 | World Journal of Stem Cells2014，6，2： | 2 |
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