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| 1 | Optimization of Culture Components for Laccase Activity from Pleurotus Ostreatus P40 Using Response Surface Methodology显示文摘High enzymatic activity is required for laccase applications.Central composite design(CCD)-based response surface methodology(RSM)can effectively increase the enzymatic activity of Pleurotus ostreatus P40 in liquid substrate fermentation.Initial screening of the nutritional components was performed using a Plackett-Burman design.The variables,namely,bran,bagasse,Tween 80,and yeast extract,were found to have statistically significant effects on laccase activity.These variables were further optimized using CCD-based RSM.Optimal concentrations for the maximum laccase activity were 8.144 2 g/L bran,50 g/L bagasse,0.424 1 mL/L Tween 80,and 2.832 5 g/L yeast extract.Under optimized conditions,the maximum measured laccase activity reached 96 480 U/L,which was close to the predicted value(104 830 U/L)by RSM.Therefore,RSM can be used to optimize culture components for laccase activity from Pleurotus ostreatus P40. | 任大军 王玲 谢凯 鲍欣 张淑琴 张惠灵 吴高明 | 2013 | Journal of Donghua University(English Edition)2013，30，4： | 1 |
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