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1Responses of Drought-Resistant Mutant vem1 to Stress and Cloning of VEM1 Gene in Arabidopsis显示文摘[Objective]This study aimed to investigate the responses of drought-resistant mutant vem1 to stress and clone VEM1 gene in Arabidopsis. [Method] A drought-resistant mutant vem1 was isolated from the Arabidopsis mutant pool. The germination rates of wild-type( WT) and mutant vem1 were detected to investigate the responses of mutant vem1 to mannitol,NaCl and ABA stress. [Result] The mutant vem1 was resistant to mannitol and NaCl stress but sensitive to ABA stress. VEM1 gene was cloned by Tail-PCR technology and sequenced. The sequencing result was submitted to NCBI for sequence alignment and gene mapping using BLAST. Database analysis suggested that VEM1 gene was a transposable element gene. [Conclusion]This study laid the foundation for functional analysis of drought-resistant gene VEM1.Junxin SHI Jiaojiao ZHANG Ziping CHEN Xiaohui DING Jie YANG Li JIANG 2013Agricultural Biotechnology2013,2,5:0
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