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| 1 | Establishment of a fluorescent polymerase chain reaction method for the detection of the SARS-associated coronavirus and its clinical application显示文摘Objective To establish a fluorescent polymerase chain reaction (F PCR) method for detecting the coronavirus related to severe acute respiratory syndrome (SARS) and to evaluate its value for clinical application Methods The primers and the fluorescence labeled probe were designed and synthesized according to the published sequence of the SARS associated coronavirus genes A F PCR diagnosis kit for detecting the coronavirus was developed, and 115 clinical nasopharyngeal gargling liquid samples were tested Results The sequence of PCR amplified products completely matched the related sequence of the SARS associated coronavirus genome Forty nine out of 67 samples from identified SARS patients and 8 of 18 samples from persons having close contact with SARS patients showed positive results All 30 samples from healthy controls were negative Conclusion The F PCR method established may be a rapid, accurate and efficient way for screening and for the early diagnosis of SARS | 吴新伟 程钢 狄飚 尹爱华 何蕴韶 王鸣 周新宇 何丽娟 罗凯 杜琳 | 2003 | Chinese Medical Journal2003，，7： | 9 |
| 2 | Melatonin及其衍生物作为急性严重呼吸系统综合征急救用药的理论基础和临床应用的可行性显示文摘已有研究资料表明 ,Melatonin作为一种维生素样内源物质 ,具有多方面的生理作用和极强的抗炎、抗自由基作用 ,在组织损伤方面可以发挥保护作用。近来实验研究证明 ,Melatonin对急性呼吸综合征具有改善症状作用 ,在Melatonin基础上新合成的具有强大抗氧化作用的衍生物也表现良好的作用。 | 谭敦宪 霍玉书 杜冠华 张均田 | 2003 | 中国药理学通报2003，19，7： | 6 |
| 3 | A hospital outbreak of severe acute respiratory syndrome in Guangzhou,China显示文摘Objective To describe a hospital outbreak of severe acute respiratory syndrome (SARS) and summarize its clinical features and therapeutic approaches.MethodsThe outbreak started with a SARS patient from the community, and a total of 96 people (76 women and 20 men, mean age (29.5±10.3) years, 93.8% of whom were health care workers) who had exposure to this source patient became infected in a short time. Clinical data in this cohort ere collected prospectively as they were identified.Results(1) The incubation period ranged from 1 to 20 (mean: 5.9±3.5) days. The duration of hospitalization was (17.2±8.0) days. (2) The initial temperature was (38.3±0.6)℃, while the highest was (39.2±0.6)℃ (P<0.001), with fever duration of (9.0±4.2) days. (3) Other most common symptoms included fatigue (93.8%), cough (85.4%), mild sputum production (66.7%), chills (55.2%), headache (39.6%), general malaise (35.4%) and myalgia (21.9%). (4) The radiographic changes were predominantly bilateral in the middle or lower lung zones. The number of affected lung fields was 1.2±0.8 on presentation, which increased to 2.9±1.4 after admission (P<0.001). The interval from the eginning of fever to the onset of abnormal chest radiographs was (3.5±2.3) days, which increased in size, extent, and severity to the maximum (6.7±3.5) days later. The time before the lung opacities were basically absorbed was (14.9±7.8) days. (5) Leukopenia was observed In 67.7% of this cohort. The time between the onset of fever and leukopenia was (4.4±2.3) days, with the lowest white blood cell count of (2.80±0.72)×109/L. (6) The lowest arterial oxygen saturation was (94.8±3.1)% with supplementary oxygen. (7) Antibiotical therapies included tetracyclines (91.0%), aminoglycosides (83.3%), quinolones (79.2%); 18.8% of the patients received a combination of tetracyclines and aminoglycosides, while 11.5% received a combination of tetracyclines and quinolones, and 63.5% received a combination of tetracyclines, aminoglycosides and quinolones. Vancomycin was used in 13.5% of the patients. (8) 68.8% of the patients were treated with methylprednisolones for a mean interval of (4.9±2.4) days. The initial dose was (67.3±28.2) mg/d and the maximal dose was (82.4±30.5) mg/d. (9) Human γ-globulin, interferon-α, antiviral drugs (oral ribavirin or oseltamivir) were used respectively in 68.6%, 46.9% and 92.7% of the patients. (10) Ninety-five patients (99.0%) had a complete clinical recovery, and only 1 patient (1.0%) died.Conclusions SARS appears to be quickly infectious and potentially lethal among health care workers, characterized by acute onset and rapid progression, and mostly bilateral lung involvement on chest radiographs. Proper administration of glucocorticosteroids seems to be of some benefits. Antibiotics, human γ-globulin, interferon-α, and antiviral drugs, although empirically, might be useful to shorten the clinical course. | 伍卫 王景峰 刘品明 陈为宪 尹松梅 江山平 严励 詹俊 陈锡龙 李建国 黄子通 黄洪章 | 2003 | Chinese Medical Journal2003，，6： | 4 |
| 4 | SARS冠状病毒的生物学和治疗学进展显示文摘波及全球众多国家的严重急性呼吸道综合征(SARS)经初步鉴定是由新型冠状病毒引起的,了解冠状病毒的生物学和免疫学特点及其进化机制,有助于对SARS的预防和治疗提供有益启示。 | 邓杨梅 谢强敏 季强 卞如濂 | 2003 | 世界临床药物2003，24，7： | 3 |
| 5 | SARS病毒抗体第一代参考品的研制及初步应用显示文摘目的 建立严重急性呼吸综合征 (SARS)病毒抗体参考品 ,用于对SARS病毒抗体诊断试剂的实验室评价。方法 收集临床确诊为SARS病人血清和非SARS人群血清 ,用不同的SARS病毒抗体诊断试剂进行检测筛查 ,选择不同样品组成参考品。并用该参考品对不同试剂进行初步评价。结果 SARS病毒IgM抗体的参考品由 38份组成 ,其中包括 11份阳性参考品、2 0份阴性参考品、灵敏度样品 6份 ,精密性样品 1份 ;SARS病毒总抗体 (包括IgG)由 4 6份组成 ,其中包括 18份阳性参考品、2 0份阴性参考品、灵敏度样品 7份 ,精密性样品 1份。结论 初步应用结果显示该参考品确实能够反应不同试剂的质量差异。 | 王佑春 李秀华 貌盼勇 毕胜利 许文波 宋爱京 | 2003 | 中华微生物学和免疫学杂志2003，23，10： | 1 |
| 6 | Dissection of SARS Coronavirus Spike Protein into Discrete Folded Fragments显示文摘The spike protein of the severe acute respiratory syndrome coronavirus (SARS-CoV) mediates cell fusion by binding to target cell surface receptors. This paper reports a simple method for dissecting the viral protein and for searching for foldable fragments in a random but systematic manner. The method in-volves digestion by DNase I to generate a pool of short DNA segments, followed by an additional step of re-assembly of these segments to produce a library of DNA fragments with random ends but controllable lengths. To rapidly screen for discrete folded polypeptide fragments, the reassembled gene fragments were further cloned into a vector as N-terminal fusions to a folding reporter gene which was a variant of green fluorescent protein. Two foldable fragments were identified for the SARS-CoV spike protein, which coincide with various anti-SARS peptides derived from the hepated repeat (HR) region 2 of the spike protein. The method should be applicable to other viral proteins to isolate antigen or vaccine candidates, thus providing an alternative to the full-length proteins (subunits) or linear short peptides. | 李爽 蔡真 陈勇 林章凛 | 2006 | Tsinghua Science and Technology2006，11，4： | 0 |
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