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| 1 | Application of response surface methodology in optimizaing thesulfation-roasting-leaching process of nickel laterite显示文摘Nickel was recovered from nickel laterite using a sulfation-roasting-leaching process and the effects of operation parameters in-cluding acid addition,roasting temperature,and roasting time on nickel extraction and iron dissolution were investigated using response surface methodology(RSM).Two second-order polynomial models of high significance were presented to show the relationship between the responses and the variables.The analysis of variance(ANOVA) showed high coefficients of determination(R2) of 0.894 and 0.980 for the two models,respectively.Optimum areas of ≥80% Ni extraction and ≥5% Fe dissolution were obtained by the overlaid contours.Verification experiments in the optimum areas were conducted and the results indicate a close agreement with the predicted values obtained from the models. | Xue-yi Guo Dong Li Zhan Wu Qing-hua Tian | 2012 | International Journal of Minerals,Metallurgy and Materials2012，19，3： | 4 |
| 2 | 联合应用错配PCR和交替延伸PCR法提高抗TNFα抗体亲和力显示文摘目的 :通过突变方法提高抗TNFα单链抗体pscTNF的亲和力。方法 :以pscTNF质粒为模板 ,应用错配PCR构建突变抗体库 ,筛选亲和力得到改善的变种 ,再以所获变种为模板 ,用交替延伸PCR技术 ,再次构建突变库 ,筛选活性得到改良的克隆。以斑点ELISA方法及硫氰酸盐洗脱ELISA法评估其亲和力的改善。结果 :从错配PCR抗体库中筛选得到 7个活性有所增强的变种 ,再通过交替延伸PCR使这 7个变种的突变位点交换重组 ,获得了亲和力进一步提高的克隆。结论 :联合应用错配PCR和交替延伸PCR法可提高单链抗体亲和力。 | 汪保安 陈晓穗 乔媛媛 曲佳 王欲晓 王琰 | 2004 | 中国免疫学杂志2004，20，12： | 0 |
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