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| 1 | 一种特定设计的富勒烯寡聚核苷酸对PCR的抑制作用显示文摘以 HeLa 细胞β-actin cDNA 的聚合酶链式反应(PCR)分析为实验模型,比较研究了针对β-actin cDNA 序列设计合成的一种富勒烯寡核苷酸(FONC)和4种其他不同侧链结构的富勒烯衍生物——三丙二酸富勒烯(TMA C_(60))、富勒醇(fullerol)、二加成亚甲基富勒烯二膦酸酯(BMPF)及单加成亚甲基富勒烯二膦酸(MMPAF)对 PCR 的作用,发现在实验条件下,这5种化合物均能抑制 PCR,且存在浓度依赖性,但以 FONC 的活性最强,它们的活性比较结果是 FONC>TMA C_(60)>fullerol>MMPAF>BMPF。加入活性氧清除剂甘露醇、叠氮化钠和 L-抗坏血酸-2-磷酸酯镁不能逆转 fullerol 却能逆转 FONC 的抑制作用。实验结果提示,这种特定设计的 FONC 对 PCR 具有较强的抑制活性,其作用机理不同于其他富勒烯衍生物。实验结果为进一步研究 FONC 的潜在应用提供了依据。 | 孟宪梅 李博 陈哲 杨新林 | 2008 | 高技术通讯2008，18，9： | 1 |
| 2 | 富勒烯对PCR反应的抑制作用显示文摘以110bp单链DNA为模板,研究富勒烯(C60)对聚合酶链式反应(polymerase chain reaction,PCR)的影响.实验结果表明,随着C60浓度的增加,PCR反应被显著抑制;将Taq DNA聚合酶、单链DNA模板与C60孵育后,其PCR扩增产物均显著减少;增加PCR反应体系中的Taq DNA聚合酶量,可消除C60的抑制作用,但增加起始单链DNA模板的数量,效应不明显.上述研究结果说明,C60不仅可抑制Taq DNA聚合酶活性,同时对DNA模板也具有一定的损伤作用. | 张捷 林燕 汪畅 梁勇 江桂斌 周群芳 | 2008 | 环境科学学报2008，28，12： | 0 |
| 3 | Inhibition of DNA restrictive endonucleases by aqueous nanoparticle suspension of methanophosphonate fullerene derivatives and its mechanisms显示文摘Aqueous nanoparticle suspension of fullerene and its derivatives are currently attracting much attention. To determine the effects of aqueous nanoparticle suspension of a mono-methanophosphonate fullerene and bis-methanophosphonate fullerene (denoted as n-MMPF and n-BMPF, respectively) on the activities of DNA restrictive endonucleases, plasmid pEGFP-N1 was cleaved at a single but differently restrictive site by EcoR I, BamH I, and isozymes Cfr9 I and Xma I, respectively. Both n-MMPF and n-BMPF inhibited the activity of EcoR I, while n-BMPF exhibited stronger inhibition than n-MMPF. Addition of n-BMPF into reaction mixtures inhibited the activities of all the four enzymes, and IC50 values for EcoR I, BamH I, Cfr9 I and Xma I were 4.3, >30, 11.7 and 8.3 μmol/L, respectively. When EcoR I was completely inhibited by n-BMPF, addition of excess amounts of pEGFP-N1 could not produce the product linear plasmid; however, increase of EcoR I amounts antagonized EcoR I inhibition of n-BMPF. Two scavengers of reactive oxygen species (ROS), mannitol and sodium azide at the concentrations of 2-10 mmol/L, did not reverse inhibition of n-BMPF, implying that this inhibition probably is not correlated to ROS. These results suggested that aqueous nano-fullerenes might act as inhibitors of DNA restrictive endonucleases. | SONG GaoGuang YAO Lu HUANG Cheng XIE Xin TAN Xin YANG XinLin | 2009 | Science China Chemistry2009，52，5： | 0 |
| 4 | 富勒烯(C_(60))对RNA逆转录的影响显示文摘目的:研究富勒烯(C60)对RNA逆转录(Reverse Transcription,RT)反应的影响。方法:以斑马鱼鱼肝mRNA为模板,在M-MLV逆转录酶作用下合成cDNA,并在RT反应体系中添加不同浓度的C60,分别研究C60对M-MLV逆转录酶和RNA构象的影响。结果:100μg/mL C60可显著抑制RT反应,抑制效应与C60浓度相关,但增加M-MLV逆转录酶量可以有效逆转C60对RT的抑制作用。C60与RNA在37℃避光孵育12 h后,C60可特异降解28S rRNA,同样具有浓度依赖性。结论:C60不仅可显著抑制M-MLV逆转录酶活性,而且对RNA也具有一定的损伤作用,C60具有潜在的基因毒性。 | 李佳 林燕 陈敏杰 张捷 李正 梁勇 | 2013 | 江汉大学学报(自然科学版)2013，41，6： | 0 |
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