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| 1 | Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection显示文摘AIM:To investigate whether tissue samples processed by the rapid urease test(RUT)kit are suitable for dualpriming oligonucleotide-based multiplex polymerase chain reaction(DPO-PCR)to detect Helicobacter pylori(H.pylori).METHODS:A total of 54 patients with specific gastrointestinal symptom were enrolled in this study.During endoscopy,gastric biopsy specimens were taken for histology,RUT,and DPO-PCR.DPO-PCR was performed on gastric biopsy samples and tissue samples that were analyzed by RUT at 2 separate institutes.In detecting H.pylori,the concordance rate of the DPO-PCR tests between the tissue samples that had been submitted to RUT and the gastric biopsy samples was investigated.RESULTS:H.pylori co-occurred with 76.0%(19/25)of gastric ulcers,64.3%(9/14)of duodenal ulcers,and 33.3%(4/12)of gastritis cases.H.pylori infection was found in 100%(3/3)of the patients with both gastric and duodenal ulcers.Overall,H.pylori was detected in 35 of 54(64.8%)patients.The diagnostic sensitivities of histology,RUT,and DPO-PCR were85.7%(30/35),74.3%(26/35),and 97.1%(34/35),respectively(P=0.02).The positive predictive value(PPV)of DPO-PCR was 94.4%,whereas the negative predictive value(NPV)was 94.7%.In the rapid urease test(CLOtest)-negative cases,the frequency of positive DPO-PCR and histologic results was 20.0%(7/35).The concordance rate of the DPO-PCR tests between the tissue samples from the RUT kit and the gastric biopsy samples was 94.4%(51/54).The rate of DPOPCR and silver stain positivity in the RUT-negative cases was 20.0%(7/35).CONCLUSION:In diagnosing H.pylori infection,DPO-PCR can be performed on tissue samples that have been processed by the RUT kit.Particularly,in patients with RUT-negative results,DPO-PCR on these tissue samples could be helpful in detecting of H.pylori infection. | Woo Chul Chung Sung Hoon Jung Jung Hwan Oh Tae Ho Kim Dae Young Cheung Byung Wook Kim Sung Soo Kim Jin Il Kim Eun Young Sin | 2014 | World Journal of Gastroenterology2014，20，21： | 10 |
| 2 | Antibiotics resistance rate of Helicobacter pylori in Bhutan显示文摘AIM:To survey the antibiotic resistance pattern of Helicobacter pylori(H.pylori)strains isolated from Bhutanese population.METHODS:We isolated 111 H.pylori strains from the gastric mucosa of H.pylori-infected patients in Bhutan in 2010.The Epsilometer test was used to determine the minimum inhibitory concentrations(MICs)of amoxicillin(AMX),clarithromycin(CLR),metronidazole(MNZ),levofloxacin(LVX),ciprofloxacin(CIP),and tetracycline(TET).RESULTS:Nineteen of the isolated H.pylori strains were susceptible to all antibiotics tested.The isolated strains showed the highest rate of antibiotic resistance to MNZ(92/111,82.9%).Among the 92 MNZresistant strains,74 strains(80.4%)showed high-level resistance(MIC≥256 g/mL).Three strains were resistance to LVX(2.7%).These strains were also resistance to CIP.None of the strains showed resistance to CLR,AMX and TET.CONCLUSION:CLR-based triple therapy is a more effective treatment approach over MNZ-based triple therapy for H.pylori infection in Bhutan. | Ratha-korn Vilaichone Yoshio Yamaoka Seiji Shiota Thawee Ratanachu-ek Lotay Tshering Tomohisa Uchida Toshio Fujioka Varocha Mahachai | 2013 | World Journal of Gastroenterology2013，19，33： | 6 |
| 3 | 不同剂量三联疗法根除幽门螺杆菌疗效比较显示文摘目的观察不同剂量三联方案7日疗法根除幽门螺杆菌(H.pylori)的疗效。方法收集216例H.pylori阳性的消化性溃疡或慢性胃炎患者,随机分为4组:RCA(小剂量)组、RCM(小剂量)组、RCA组及RCM组分别给予含克拉霉素0.25及0.5的三联方案治疗7 d,各组慢性胃炎患者疗程结束后停用所有药物,消化性溃疡患者停用抗生素后继续口服雷贝拉唑4~6周。所有受试者停用抗生素至少4周并停用PPI至少2周后复查14C-UBT。结果①4组H.pylori根除率按ITT分析分别为77.78%、55.56%、83.33%及55.56%,按PP分析分别为82.35%、65.95%、90.00%及68.18%,RCA组H.pylori根除率高于RCA(小剂量)组差异有显著性(P<0.05);②4组患者溃疡愈合情况、症状缓解率及不良反应发生率均差异无显著性(P>0.05);③RCA(小剂量)组、RCM(小剂量)组较RCA组及RM组方案治疗成本及C/E比值低,RCA(小剂量)组和RCA组三联7日疗效优于RCM(小剂量)组及RCM组,能有效根除H.pylor(iP<0.05),RCA组优于RCA(小剂量)组(P<0.05)。结论 RCA(小剂量)组和RCA组三联7日疗法均能有效根除H.pylori,且副反应小,病人依从性好,且RCA(小剂量)组及RCA组有望成为一线H.pylori根除方案,尤其是RCA组。 | 唐艳波 谢胜 林中 | 2012 | 中国现代医学杂志2012，22，13： | 6 |
| 4 | 噬菌体治疗耐药性幽门螺杆菌的研究进展显示文摘幽门螺杆菌感染是胃癌重要致病因子。目前,幽门螺杆菌对抗生素耐药情况日趋普遍。噬菌体治疗作为一种生物疗法在治疗幽门螺杆菌方面有极大的潜力。本文就噬菌体治疗耐药性幽门螺杆菌的现状及趋势进行综述。 | 熊婧 白杨 | 2011 | 胃肠病学和肝病学杂志2011，20，6： | 3 |
| 5 | 幽门螺杆菌抗原基因ureB在乳酸菌中的克隆表达与免疫反应性研究显示文摘目的为了开发幽门螺杆菌(Helicobacter pylori,Hp)口服疫苗,将Hp尿素酶B(UreB)在食品级乳酸乳球菌NZ3900菌株中进行表达,并研究其免疫反应性。方法 PCR扩增Hp MEL-HP27菌株ureB基因(基因号:FJ436980),将其克隆入大肠杆菌-乳酸乳球菌穿梭质粒pNZ8110中并转化乳酸乳球菌NZ3900;采用正交试验确定目的蛋白表达的适宜条件;应用western-blot鉴定其免疫反应性。结果成功扩增了Hp MEL-HP27菌株ureB基因,构建了ureB基因的乳酸菌NICE(Nisin-controlled expression)原核表达系统;UreB蛋白适宜的表达条件为:在ureB重组菌生长至对数生长前期(OD600≈0.3~0.4)加入终浓度为40ng/mL的nisin,诱导表达5h,可溶性UreB蛋白表达量最高,可达27.26μg/mL培养基。可溶性UreB蛋白的表达占上清蛋白的比例最高可达20.19%;Western-blot结果显示乳酸乳球菌表达的UreB抗原蛋白具有良好的免疫反应性。结论结果提示应用乳酸乳球菌构建幽门螺杆菌食品级疫苗可能具有较好前景。 | 张小娟 张荣光 段广才 范青堂 | 2011 | 中国人兽共患病学报2011，27，10： | 3 |
| 6 | 不同剂量三联疗法根除幽门螺杆菌疗效比较显示文摘目的观察不同剂量三联方案7日疗法根除幽门螺杆菌(H.pylori)的疗效。方法收集216例H.pylori阳性的消化性溃疡或慢性胃炎患者,随机分为4组:RCA(小剂量)组、RCM(小剂量)组、RCA组及RCM组分别给予含克拉霉素0.25及0.5的三联方案治疗7d,各组慢性胃炎患者疗程结束后停用所有药物,消化性溃疡患者停用抗生素后继续口服雷贝拉唑4~6周。所有受试者停用抗生素至少4周并停用PPI至少2周后复查14C—UBT。结果①4组H.pylori根除率按ITT分析分别为77.78%、55.56%、83.33%及55.56%,按PP分析分别为82.35%、65.95%、90.00%及68.18%,RCA组H.pylori根除率高于RCA(小剂量)组差异有显著性(P〈0.05);②4组患者溃疡愈合情况、症状缓解率及不良反应发生率均差异无显著性(P〉0.05);③RCA(小剂量)组、RCM(小剂量)组较RCA组及RM组方案治疗成本及C/E比值低,RCA(小剂量)组和RCA组三联7日疗效优于RCM(d,剂量)组及RCM组,能有效根除H.pylori(P〈0.05),RCA组优于RCA(小剂量)组(P〈0.05)。结论RCA(小剂量)组和RCA组三联7日疗法均能有效根除H.pylori,且副反应小,患者依从性好,且RCA(小剂量)组及RCA组有望成为一线H.pylori根除方案,尤其是RCA组。 | 唐艳波 谢胜 林中 | 2014 | 柳州医学2014，27，2： | 1 |
| 7 | 幽门螺杆菌根除效果的影响因素分析显示文摘目的分析幽门螺杆菌(H.pylori)根除效果的影响因素。方法将接受规律H.Pylori根除治疗的1 034例患者分为成功组和失败组,比较两组患者的一般情况和治疗方案,分析根除H.pylori治疗效果的影响因素。结果H.Pylori根除率为75.0%,初治组为75.7%,复治组为64.5%,两组比较差异有统计学意义(P<0.05)。初治患者中,四联治疗组的根除率高于三联治疗组和序贯治疗组(P<0.01或P<0.05),7、10和14 d治疗的根除率分别为75.4%、71.6%和91.5%;应用质子泵抑制剂(PPI)者的根除率高于未应用者(P<0.01)(81.0%vs 63.0%);7 d四联初治方案中,阿莫西林联合克拉霉素方案组的根除率优于阿莫西林联合甲硝唑/替硝唑方案组(P<0.01)(94.2%vs 75.5%)。结论四联治疗方案的H.pylori根除率高于三联治疗。延长治疗时间和应用PPI有利于提高H.pylori根除率。PPI和铋剂联合阿莫西林、克拉霉素的根除率较高。 | 冯璜 | 2011 | 苏州大学学报(医学版)2011，31，4： | 0 |
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