|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Update on occult hepatitis B virus infection显示文摘The event of mutations in the surface antigen gene of hepatitis B virus(HBV) results in undetectable hepatitis B surface antigen with positive/negative anti-hepatitis B core(anti-HBc) antibody status in serum and this phenomenon is named occult hepatitis B infection(OBI). The presence of anti-HBc antibody in serum is an important key for OBI tracking, although about 20% of OBI cases are negative for anti-HBc antibody. The diagnosis of OBI is mainly based on polymerase chain reaction(PCR) and real-time PCR assays. However, real-time PCR is a more reliable method than PCR. OBI is a great issue for the public health problem and a challenge for the clinical entity worldwide. The persistence of OBI may lead to the development of cirrhosis and hepatocellular carcinoma. With regard to OBI complications, the screening of HBV DNA by the highly sensitive molecular means should be implemented for:(1) patients with a previous history of chronic or acute HBV infection;(2) patients co-infected with hepatitis C virus/human immunodeficiency virus;(3) patients undergoing chemotherapy or anti-CD20 therapy;(4) recipients of organ transplant;(5) blood donors;(6) organ transplant donors;(7) thalassemia and hemophilia patients;(8) health care workers;(9) patients with liver related disease(cryptogenic);(10) hemodialysis patients;(11) patients undergoing lamivudine or interferon therapy; and(12) children in time of HBV vaccination especially in highly endemic areas of HBV. Active HBV vaccination should be implemented for the close relatives of patients who are negative for OBI markers. Thus, the goal of this review is to evaluate the rate of OBI with a focus on status of high risk groups in different regions of the world. | Manoochehr Makvandi | 2016 | World Journal of Gastroenterology2016，22，39： | 28 |
| 2 | Clinical significance of occult hepatitis B virus infection显示文摘Occult hepatitis B virus(HBV) infection(OBI) is defined as the presence of HBV DNA in the liver(with or without detectable HBV DNA in serum) for individuals testing HBV surface antigen negative.Until recently,the clinical effect of OBI was unclear on the progression of liver disease;on the development of hepatocellular carcinoma;and on the risk for reactivation or transmission of HBV infection.Several studies suggest a high prevalence of OBI among patients with cryptogenic chronic liver disease,but its role in the progression to cirrhosis remains unclear.Although OBI has been well documented in human immunodeficiency virus(HIV) -positive patients,especially among those coinfected with hepatitis C virus,further studies are needed to determine its current clinical impact in HIV setting. | Miriam Romero Antonio Madejón Conrado Fernández-Rodríguez Javier García-Samaniego | 2011 | World Journal of Gastroenterology2011，17，12： | 6 |
| 3 | 150例不明原因肝功能异常患者的临床特点及病因分析显示文摘目的通过肝脏组织病理学检查,对150例不明原因肝功能异常患者临床特点、病因进行分析。方法回顾性选取2010年12月~2015年12月我院不明原因肝功能异常患者150例,对肝脏组织病理结果,临床特点等病例资料进行统计分析。结果通过对150例患者肝脏组织病理结果分析,最终明确病因的患者共139例,仍有11例患者未明确诊断。经病理学明确的病因有以下几类:自身免疫性肝病43例,约占28.67%,包括AIH患者16例(10.67%)、PBC患者24例(16.00%)、重叠综合征(AIH+PBC)3例(2.00%);药物性肝炎44例(29.33%);非酒精性脂肪性肝炎35例(23.33%);酒精性肝病14例(9.33%);遗传代谢性肝病3例(2.00%),包括遗传性血色病1例(0.67%)、肝豆状核变性2例(1.33%)。结论不明原因肝功能异常病因中以自身免疫性肝病、药物性肝损伤最为常见,其次为非酒精性及酒精性脂肪肝。肝组织病理活检对不明原因肝功能异常患者诊断价值较高,但也存在一定局限性,重视临床资料收集,通过临床及病理特点相结合的方法,有助于进一步明确诊断。 | 杨文杰 | 2016 | 中国卫生标准管理2016，7，4： | 3 |
| 4 | ALT正常、HBsAg阴性人群中隐匿型乙型肝炎病毒的感染情况显示文摘目的了解隐匿型乙型肝炎病毒(occult HBV)在ALT正常、HBsAg阴性人群中的感染情况。方法应用荧光定量聚合酶链反应(FQ-PCR)方法检测192例ALT正常、HBsAg阴性人群血清中HBV-DNA含量。结果192例人群中HBV-DNA阳性率为15.6%(30/192),HBV-DNA含量均低于103.6copies/ml。男性和女性人群的HBV-DNA阳性率分别为22.8%(23/101)和7.7%(7/91),差异有显著性(2=8.26,P<0.01);50岁以上和50岁以下人群的HBV-DNA阳性率分别为17.6%(3/17)和15.4%(27/175),两者差异无显著性(2=0.06,P>0.05);抗-HBe阳性和(或)抗-HBc阳性和(或)抗-HBs阳性与HBV标志物(HBV-M)全阴性人群的HBV-DNA阳性率分别为18.2%(28/154)与5.3%(2/38),差异有显著性(2=3.86,P<0.05)。结论ALT正常人群中存在隐匿型HBV感染,且与性别有关。提示临床在输血和器官移植时,应结合灵敏度高的HBV-DNA检测方法,方能预防隐匿型HBV的传播。 | 赵友云 高应林 王春香 乐惠荣 | 2007 | 广东医学2007，28，7： | 3 |
| 5 | 69例不明原因肝功异常患者血清HBV痕量基因检测及其阳性结果分析显示文摘目的检测不明原因肝功能异常患者(伴各型乙型肝炎病毒抗体阳性)的隐匿性乙型肝炎病毒感染情况,探讨其临床特征。方法用巢式PCR扩增69例不明原因肝功异常患者的血清HBV C、S、X基因片段,并分析与总结隐匿性乙型肝炎病毒感染的临床、生化和血清学特点。结果 69例不明原因肝损害患者隐匿性乙型肝炎病毒感染检出13例,检出率为18.84%,以大于40岁的男性为主。在检出的13例患者中,10例(76.92%)仅有轻微乏力、食欲减退症状,9例(69.23%)总胆红素值正常或轻度升高,丙氨酸氨基转移酶有不同程度的异常,10例(76.92%)隐匿性乙型肝炎病毒感染的血清学模式为HBs Ab(-)/HBc Ab(+)/HBe Ab(±)。结论乙型肝炎病毒的隐匿性感染是不明原因肝功异常的原因之一,其所致的肝炎临床症状轻微,血清学模式呈多样性,生化以不同程度的转氨酶升高为主。 | 袁琴 刘飞飞 罗旗 刘何蕾 李成敏 郑洁 曾爱中 | 2015 | 第三军医大学学报2015，37，14： | 1 |
| 6 | 隐源性肝炎相关新基因CHBP2的原核表达及蛋白纯化显示文摘目的:构建隐源性肝炎相关新基因CHBP2原核表达载体,在大肠杆菌中进行表达,并纯化CHBP2融合蛋白。方法:应用逆转录聚合酶链反应(RT-PCR),以提取的Huh7细胞mRNA为模板,扩增获得CHBP2基因片段,连接到pGEM-T载体,测序正确后插入至原核表达载体pET-32a(+)中,构建原核表达载体pET-32a(+)-CHBP2,转化大肠杆菌BL21,以异丙基-β-D-硫代半乳糖苷(IPTG)诱导,获得CHBP2融合蛋白的可诱导性表达,通过SDS-PAGE电泳、Western blot免疫印迹分析证实蛋白表达的特异性,超声破碎表达细菌,SDS-PAGE分析,利用镍离子亲和柱对表达蛋白进行纯化及柱上复性。结果:成功构建原核表达载体pET-32a(+)- CHBP2,并将CHBP2融合蛋白成功表达,通过Western blot免疫印迹,证实了蛋白表达的特异性。SDS-PAGE分析表明其为包涵体表达,并对蛋白成功进行了纯化和复性,获得了表达蛋白的纯品结论:成功表达、纯化CHBP2蛋白,为研究CHBP2蛋白的生物学功能打下了基础, | 李锟 叶进 肖凡 李国力 洪源 魏红山 | 2007 | 世界华人消化杂志2007，15，22： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员